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A dendrimer-encapsulated gold nanoparticle probe and its preparation and application

A technology of nano-gold particles and dendrimers, which is applied in the field of functional nanoparticles and its preparation and application, to achieve good water solubility and stability, easy operation and separation, and good development prospects

Active Publication Date: 2021-08-31
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Searching domestic and foreign literatures has not found any research on the use of functionalized dendrimers to encapsulate gold nanoparticles and compound calcium ion probes for T cell CT / fluorescence dual-modality imaging

Method used

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  • A dendrimer-encapsulated gold nanoparticle probe and its preparation and application
  • A dendrimer-encapsulated gold nanoparticle probe and its preparation and application
  • A dendrimer-encapsulated gold nanoparticle probe and its preparation and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] (1) Dissolve 22.7 mg of polyethylene glycol (MAL-PEG-OH) whose end groups are maleimide and hydroxyl respectively in 2 mL of ultrapure water, and add the fifth-generation polyamidoamine whose end is amino Dendrimers (G5.NH 2 ) aqueous solution (12.3mg G5.NH 2 dissolved in 1mL ultrapure water) aqueous solution, reacted overnight, purified by dialysis, and freeze-dried the reaction solution to obtain dry PEGylated dendrimers (G5.NH 2 -PEG-OH) powder;

[0059] (2) Get the G5.NH prepared in step (1) 2 -Dissolve 10.0 mg of PEG-OH in 1 mL of ultrapure water, and add 66.3 μL of chloroauric acid (HAuCl 4 ) aqueous solution (concentration is 30mg / mL), stir evenly, then quickly add 92.0μL sodium borohydride (NaBH 4 ) aqueous solution (concentration: 10mg / mL), fully reduce for 3h. Purified by dialysis, and the reaction solution was freeze-dried to obtain dry dendrimer-coated gold nanoparticles ({(Au 0 ) 25 -G5.NH 2 -(PEG-OH) 14}DENPs) powder;

[0060] (3) get {(Au) prepa...

Embodiment 2

[0063] Get the G5.NH prepared in Example 1 2 -PEG-OH, {(Au 0 ) 25 -G5.NHAc-(PEG-OH) 14}DENPs and {(Au 0 ) 25 -G5.NHAc-(PEG) 14 -(Fluo-4) 2}DENPs were dissolved in deuterated water, and Bruker 400MHz nuclear magnetic resonance was used for hydrogen spectrum testing. The characterization results are shown in the appendix of the manual. figure 2 , figure 2 In (a), G5.NH appears at 2.3-3.4ppm 2 The methylene characteristic peak of PEG, the methylene characteristic peak of PEG appears at 3.7ppm; figure 2 In (b), after the acetylation reaction, the methyl characteristic peak of the acetyl group appeared at 1.88ppm; figure 2 In (c), the proton peak of the aromatic ring of Fluo-4 appeared in the range of 6-8ppm, calculated according to the ratio of the integral area, each G5.NH 2 13.6 MAL-PEG-OH were connected, 68 acetyl groups were modified, and 2.4 Fluo-4 probes were connected. Take the same concentration of {(Au 0 ) 25 -G5.NHAc-(PEG-OH) 14}DENPs and {(Au 0 ) 25 ...

Embodiment 3

[0069] Get the {(Au) prepared in Example 1 0 ) 25 -G5.NHAc-(PEG) 14 -(Fluo-4) 2}DENPs, configure {(Au 0 ) 25 -G5.NHAc-(PEG) 14 -(Fluo-4) 2}DENPs aqueous solution for CT scanning. See the attached manual for the results Figure 5 , Figure 5 In (a), as the concentration increases, the CT signal gradually becomes brighter, and the CT values ​​​​of different concentrations are plotted, see the attached Figure 5 (b), the X-ray attenuation coefficient has a linear relationship with its concentration.

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Abstract

The invention relates to a dendrimer-wrapped nano-gold particle probe and its preparation and application. The preparation method comprises: G5.NH 2 ‑PEG‑OH preparation, {(Au 0 ) 25 ‑G5.NH 2 -(PEG-OH) 14}DENPs preparation, {(Au 0 ) 25 ‑G5.NHAc‑(PEG‑OH) 14}DENPs preparation, {(Au 0 ) 25 ‑G5. NHAc‑(PEG) 14 -(Fluo-4) 2} DENPs preparation. The method is simple, easy to operate and separate, and the source of raw materials is commercialized, which has good development prospects; the obtained dendrimer-wrapped nano-gold particle probe has good water solubility and stability, and can be used to monitor the state of T cells. T cells are tracked by CT imaging, which has a dual-modality imaging effect.

Description

technical field [0001] The invention belongs to the field of functional nanoparticles and their preparation and application, in particular to a dendrimer-wrapped nano gold particle probe and its preparation and application. Background technique [0002] Tumor immunotherapy has received great attention in recent years. It mainly relies on the activation of the organism's own immune system to finally achieve the purpose of killing tumors by T cells. Therefore, the monitoring of T cells can provide an important basis for evaluating the efficacy of tumor immunotherapy. After research, calcium ion signals can describe the different physiological states of biological cells including T lymphocytes. As a new type of calcium ion fluorescent probe, Fluo-4 has the advantages of bright fluorescence and sensitive response. It has been reported in the literature that a Fluo-4 probes on the surface of upconverting nanoparticles were used to monitor calcium signals at different levels in l...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/04A61K49/00
CPCA61K49/0002A61K49/0019A61K49/0054A61K49/0093A61K49/04
Inventor 史向阳陈玫秀范钰
Owner DONGHUA UNIV