FDU-12-based aflatoxin surface imprinted polymer and application thereof

Abstract

The invention belongs to the field of molecularly imprinted polymers and discloses an aflatoxin surface imprinted polymer and application thereof in crop testing. A mesoporous silica material FDU-12 is selected as a carrier, 7-acetoxyl-4-methylcoumarin serves as a replacement template of aflatoxin, and a surface imprinted polymer FDU-12@MIPs is synthesized. The synthesized polymer serves as an adsorbent for preparing a surface imprinted solid-phase extraction column, the aflatoxin in foods is extracted and separated, and a rapid, high-efficiency and cheap aflatoxin sample pretreatment method is obtained. When the aflatoxin sample pretreatment method is combined with high performance liquid chromatography, a method for detecting aflatoxin in foods with advantages of low price, simple operation and low detection limit is researched and developed. The aflatoxin surface imprinted polymer disclosed by the invention is favorable for development of crop toxin detection sample pretreatment andsolves the problem that the current pretreatment method is high in cost and high in time consumption.

Description

technical field [0001] The invention belongs to the field of molecularly imprinted polymers, and relates to an aflatoxin surface imprinted polymer and its application in the detection of crops. Background technique [0002] During the process of growth, harvesting, storage and processing, crops are susceptible to fungal infection and produce aflatoxins (Aflatoxins, AFs). Some biological systems and organs in the human body have serious toxic and carcinogenic effects. The AFs produced by the metabolism of Aspergillus flavus will remain in the agricultural products. If the agricultural products are processed into food, they will cause damage to the human body after human consumption; if the agricultural products are processed into feed to feed livestock, AFs will accumulate in the livestock. When people use meat Such products are still at risk from AFs. There are many types of AFs, and there are some differences in the structures of different types of AFs. Among them, aflato...

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Problems solved by technology

[0013] Although the combination of molecular imprinting and solid phase extraction can effectively extract target molecules, traditional molecular imprinting polymers also have some disadvantages: it is not easy to completely elute the template molecules; the mass transfer rate is low; the shape of the polymer is irregular; Lower adsorption capacity, etc. (Wang Y, Yang Y, Lan X, et al.Bisphenol A sensing based on surface molecularly imprinted, ordered mesoporous silica[J], ElectrochimicaActa,2011,56(5):2105-2109.; Hongliang H, Xiaoli G, Liying S, et al. Molecularly imprinted polymers based on SBA-15 for selective solid-phase extraction of baicalein from plasma samples[J], Analytical and Bioanalytical Chemistry, 2015, 407(2):509-519.), while surface imprinted Technology can well make up for the above shortcomings

At present, there is no use of mesoporous silica FDU-12 as a carrier to prepare surface molecularly imprinted polymers for the detection of wheat toxins and other crops

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