Somatic cell culturing method for effectively promoting mongolian oak globular embryogeny

Abstract

The invention provides a somatic cell culturing method for effectively promoting mongolian oak globular embryogeny. The method is characterized by comprising the following steps of selection and treatment of an explant, disinfection for the explant, inoculation, callus induction and somatic embryo induction. The somatic cell culturing method for effectively promoting mongolian oak globular embryogeny has the following advantages that the rate of callus induction and the somatic embryo induction rate are high, and embryonic callus with a small cell volume, compact and thick cytoplasm and a large and clear cell nucleus is obtained; the problems are solved that cutting rooting of mongolian oak is difficult, the propagation coefficient is small, the speed is low, and large-scale production cannot be formed for meeting the market requirements, and by utilizing the cell totipotency, the purpose of large-scale cultivation of good plants in a short period is achieved; the possibility is provided for establishment of the asexual propagation system of the mongolian oak and industrialized seedling culturing of the mongolian oak.

Description

technical field [0001] The invention belongs to the technical field of plant cell culture, and in particular relates to a somatic cell culture method for effectively promoting globular embryogenesis of Quercus mongolica. Background technique [0002] Quercus mongolica (Quercus mongolica) is a plant of the genus Quercus (Fagaceae), also known as Quercus mongolica, Oak oak, Oak tree, and belongs to deciduous trees. It is a national second-class precious tree species in China and the main secondary forest tree species in the forest regions of Northeast China. Produced in Heilongjiang, Jilin, Liaoning, Inner Mongolia, Hebei and other provinces and regions in China. It is also distributed in Russia, North Korea, and Japan, and it is planted in many places in the world. Because of its tall tree shape, Quercus mongolica can be used as a garden tree or street tree, and those with good tree shape can be used as solitary trees for ornamental purposes. Quercus mongolica seeds have h...

AI Technical Summary

Problems solved by technology

However, due to the severe shortage of fine varieties of Quercus mongolica, the long growth cycle, late fruiting period, and low yield, the seeds appear in a big year every 3-5 years. In addition, the seeds are not resistant to storage and are easily attacked by various insects during collection and storage. All these characteristics make it difficult to establish a Quercus mongolica seed orchard

Although conventional asexual reproduction methods such as cuttings and grafting can maintain the excellent traits of the female parent for a long time, it is difficult for Quercus mongolica to take root, the reproduction coefficient is low, and the speed is slow.

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