A method for extracting zeaxanthin from corn gluten enzymolysis jelly
A technology of corn gluten powder and zeaxanthin, which is applied in the fields of chemical industry, medicine, and food, can solve the problems of long time, difficult application, and limited improvement of extraction rate, and achieve the effects of promoting transformation, increasing yield, and improving purity
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Embodiment 1
[0032] Take corn gluten powder and grind it through a 60-mesh sieve, take 100 g of the sieved corn gluten powder, add 500 mL of tap water, and mix well; adjust the pH to 7 with 30% NaOH solution, and let it soak for 3 hours. When the water bath is heated under stirring until the temperature of the feed liquid is 42°C, according to the composition of the sieved corn gluten powder and compound protease (subtilisin, streptomycin and aminopeptidase, the compound ratio is 4:3:3 (W:W:W), the three enzymes were respectively purchased from Novozymes) = 100:3 (w / w) ratio, 3 g of complex protease was added, and the enzymatic hydrolysis was performed for 24 hours. Pour out the feed solution after enzymatic hydrolysis, and dry it in an oven at 65°C for 16 hours. Crush to a fine powder. Add 500mL 95% ethanol for extraction for 12 hours, filter to obtain the first filtrate and filter residue, and set aside the filtrate, add 500mL 95% ethanol to the filter residue, extract at 55°C for 1 hou...
Embodiment 2
[0038] Take corn gluten powder and crush it through an 80-mesh sieve, take 100 g of the sieved corn gluten powder, add 400 mL of tap water, and mix well; adjust the pH to 7.2 with 10% NaOH solution, let it stand for 2 hours to soak, and obtain a feed liquid. When heating in a water bath with stirring until the temperature of the feed liquid is 38°C, add 2.5 g of the compound protease at a ratio of 100:2.5 (w / w) of the sieved corn gluten powder and the compound protease, and perform enzymatic hydrolysis for 36 hours. Pour out the feed solution after enzymolysis, and dry it in an oven at 75°C for 14 hours. Crush to a fine powder. Add 400mL of absolute ethanol, stir and extract for 8h, filter to obtain the first filtrate and filter residue, and use the filtrate for later use; add 400mL of absolute ethanol to the filter residue, extract at 65°C for 2h, filter to obtain the second filtrate and filter residue, and use the filtrate for later use; repeat the second time The extractio...
Embodiment 3
[0040] Take corn gluten powder and crush it through a 40-mesh sieve, take 100 g of the sieved corn gluten powder, add 600 mL of tap water and mix well; adjust the pH to 6.8 with 20% NaOH solution, and let it soak for 4 hours. When heating in a water bath with stirring until the temperature of the feed liquid is 45°C, add 4.5 g of compound protease according to the ratio of the sieved corn gluten powder and compound protease = 100:4.5 (w / w), enzymolyze for 12 hours, and pour out the enzyme The decomposed feed liquid was dried in an oven at 55°C for 20 hours, and then crushed into fine powder. Add 600mL of 95% ethanol, stir and extract for 4h, filter to obtain the first filtrate and filter residue, and set aside the filtrate, add 600mL of 95% ethanol to the filter residue, extract at 50°C for 1.5h, filter to obtain the second filtrate and filter residue, and set aside the filtrate. Repeat the second extraction process once, that is, heat extraction for 2 times in total, and filt...
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