Detection method for Polygonum cuspidatum-trichosanthes skin dachengqi decoction
A detection method and technology of Dachengqi Decoction, applied in the field of analysis and detection, can solve the problems of unclear component distribution, complex components, and unclear mechanism of action, and achieve the effects of accurate and reliable detection results, low detection cost, and easy control.
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Embodiment 1
[0040] The detection of embodiment 1 Hupi Dachengqi Decoction of the present invention
[0041] 1) Preparation of reference solution: Weigh polydatin, emodin, hesperidin, magnolol and vanillic acid respectively, and dilute with methanol to contain 0.98 mg of emodin, 0.97 mg of vanillic acid, 0.98 mg of hesperidin, A solution of polydatin 0.98mg and magnolol 0.98mg.
[0042] 2) Preparation of the test solution: take the medicines of Hupi Dachengqi Decoction according to the proportion of crude drugs, including 15g of Polygonum cuspidatum, 12g of rhubarb, 15g of Trichosanthes bark, 15g of Magnolia officinalis, 9g of Glauber's salt, and 12g of Citrus aurantii, after adding 50mL of 65% methanol After ultrasonic dissolving for 2 hours, centrifuge at 12000rpm and take the supernatant and filter it with 0.45μm Millipore filter paper to obtain;
[0043] 3) draw reference substance solution and need testing solution respectively and inject liquid chromatography-mass spectrometer, and ...
Embodiment 2
[0050] Embodiment 2 The detection method of Hupi Dachengqi Decoction in the blood of the present invention
[0051] 1) Preparation of the test solution:
[0052] Collect 0.35 mL of blood, centrifuge at 3500 r / min for 30 min, take 200 μL of supernatant, add 0.05 mL of 0.1mol / L HCl, 1 mL of internal standard (50% methanol), mix well, add 1 mL of ethyl acetate, mix, and centrifuge at 4°C for 5 min ( 13000rpm), transfer the supernatant to a conical bottom glass tube, place it in a water bath at 45°C to evaporate to dryness, and redissolve the residue with 100 μL acetonitrile-water (ratio 3:7).
[0053] 2) Preparation of blank plasma
[0054] Collect 200 μL of plasma that has not been fed any drugs, add 0.05mL of 0.1mol / L HCl, 1mL of internal standard (50% methanol), mix well, add 1mL of ethyl acetate, mix, centrifuge at 4°C for 5min (13000rpm), transfer to Put the supernatant in a sharp-bottomed glass tube and evaporate it to dryness in a water bath at 45°C. The residue was redi...
Embodiment 3
[0063] The detection method of tiger skin Dachengqi Decoction in the tissue of embodiment 3 of the present invention
[0064] 1) Preparation of the test solution:
[0065] Collect 100 mg of tissue, add 900 μL of normal saline and homogenate, centrifuge at 10,000 r / min for 10 min and take 200 uL of supernatant; add 0.05 mL of 0.1 mol / L HCl and 1 mL of internal standard to blood and tissue samples, mix well, and add 1 mL of ethyl acetate Ester, mixed, centrifuged at 4°C for 5min (13000rpm), transferred the supernatant to a conical bottom glass tube, evaporated to dryness in a water bath at 45°C, and redissolved the residue with 100μL acetonitrile-water (ratio 3:7).
[0066] 2) Preparation of blank plasma
[0067] Collect 200 μL of plasma that has not been fed any drugs, add 0.05mL of 0.1mol / L HCl, 1mL of internal standard (50% methanol), mix well, add 1mL of ethyl acetate, mix, centrifuge at 4°C for 5min (13000rpm), transfer to Put the supernatant in a sharp-bottomed glass tub...
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