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Method for preparing plant pure 2-octulose in small quantities

A technology of octanulose and pure product is applied in the field of small-scale preparation of pure 2-octanulose of plants, and can solve the problem that there is no relevant research report on the preparation method of pure 2-octanulose, scientific research and product development to be carried out, Limit the research and development of 2-octanulose, and achieve the effect of good application prospects, easy widespread application, and easy implementation

Active Publication Date: 2019-09-20
SOUTHWEST UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since 2-octulose is a rare sugar, its research and application have not received widespread attention, so the commercial 2-octulose pure product has not yet been developed, and there is no relevant research report on 2-octulose pure product preparation method
The lack of pure 2-octulose limits the research and development of 2-octulose
[0004] To sum up, the existing problems in the prior art are: since 2-octulose has not received widespread attention, its scientific research and product development have yet to be carried out
[0005] Difficulty in solving the above-mentioned technical problems: Generally, the content of 2-octulose in plants is relatively low. To prepare pure products, more plant materials are needed and the cost is higher , less efficient

Method used

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  • Method for preparing plant pure 2-octulose in small quantities
  • Method for preparing plant pure 2-octulose in small quantities
  • Method for preparing plant pure 2-octulose in small quantities

Examples

Experimental program
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Effect test

Embodiment 1

[0047] The fresh leaves of the revived plant Craterostigma plantagineum in normal growth were ground into powder with liquid nitrogen, and then 1 g of the powder was weighed and added to 1 mL of 80% methanol solution, and extracted by shaking for 30 min. After the extract was centrifuged at 10,000 g for 5 min, the obtained supernatant was transferred to a new centrifuge tube; the precipitate was repeatedly extracted three times with 80% methanol solution, and the supernatant obtained after centrifugation was transferred and combined into a new test tube. The obtained supernatant was evaporated to dryness under reduced pressure at 25° C. using an Eppendorf concentrator to obtain a precipitate. After the precipitate is fully dissolved in 1mL pure water, add 500μL chloroform to the aqueous solution, vortex fully, centrifuge at 10000g for 3min to separate the solution, draw the upper layer solution into a new test tube, add 500μL chloroform again, and vortex fully, Centrifuge at 1...

Embodiment 2

[0049] The fresh leaves of the revived plant Craterostigma Plantagineum in normal growth were ground into powder with liquid nitrogen, and then 5 g of the powder was weighed and added to 10 mL of 80% methanol solution, and extracted by shaking for 30 min. After the extract was centrifuged at 10,000 g for 5 min, the obtained supernatant was transferred to a new centrifuge tube; the precipitate was repeatedly extracted three times with 80% methanol solution, and the supernatant obtained after centrifugation was transferred and combined into a new test tube. The obtained supernatant was evaporated to dryness under reduced pressure at 25° C. using an Eppendorf concentrator to obtain a precipitate. After the precipitate is fully dissolved in 5mL pure water, add 2.5mL chloroform to the aqueous solution, vortex fully, centrifuge at 10000g for 3min to separate the solution, draw the upper layer solution into a new test tube, add 2.5mL chloroform again, and vortex fully Afterwards, cen...

Embodiment 3

[0051] The fresh leaves of the revived plant Craterostigma Plantagineum in normal growth were ground into powder with liquid nitrogen, and then 10 g of the powder was weighed and added to 10 mL of 80% methanol solution, and extracted by shaking for 30 min. After the extract was centrifuged at 10,000 g for 5 min, the obtained supernatant was transferred to a new centrifuge tube; the precipitate was repeatedly extracted three times with 80% methanol solution, and the supernatant obtained after centrifugation was transferred and merged into a new petri dish. The obtained supernatant was evaporated to dryness at 50° C. using a heating metal block to obtain a precipitate. After the precipitate is fully dissolved in 5mL pure water, add 2.5mL chloroform to the aqueous solution, vortex fully, centrifuge at 10000g for 3min to separate the solution, draw the upper layer solution into a new test tube, add 2.5mL chloroform again, and vortex fully Afterwards, centrifuge at 10000g for 3min ...

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Abstract

The invention belongs to the technical field of extraction and purification of plant rare monosaccharides, and discloses a method for preparing plant pure 2-octulose in small quantities. The recovery plant craterostigma plantagineum normally growing is used as a material, is extracted by a methanol solution and is steamed to dry under reduced pressure, a precipitate is re-dissolved, then impurities such as pigments, proteins, salt ions and amino acids are removed by chloroform washing, ion exchange resin adsorption and other steps, and a carbohydrate mixture is obtained. The carbohydrate mixture is separated by paper chromatography, the position of 2-octulose on the chromatographic paper is determined by developing a carbohydrate reference substance, the paper part is cut off and soaked in methanol, the soaking solution is filtered by a filter membrane and is steamed to dry under reduced pressure, and pure octulose is obtained. The invention provides the method for preparing the 2-octulose standard, and the problem that the lack of 2-octulose standard affects a subsequent research and development is solved. Compared with a method of preparative liquid chromatography, the method is simple in technology, easy to implement and low in cost.

Description

technical field [0001] The invention belongs to the technical field of extraction and purification of rare plant monosaccharides, and in particular relates to a method for small-scale preparation of pure plant 2-octulose. Background technique [0002] At present, the closest prior art: 2-octulose (2-Octulose) is a special eight-carbon monosaccharide, which exists in microorganisms, plants and animals, and is currently the most reported in plants. Although 2-octulose is widely distributed, its physiological role in plants and its role in animal nutrition metabolism are not fully understood, and corresponding scientific research is yet to be carried out. Through the research and analysis of sugars similar to 2-octulose, it can be confirmed that 2-octulose has important potential value for scientific research, animal nutrition and health care, and 2-octulose may be used in the production of food or Feed additives, nutritional health products and drugs related to glucose metabo...

Claims

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Application Information

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IPC IPC(8): C07H3/02C07H1/08
CPCC07H3/02C07H1/08
Inventor 张庆伟罗克明
Owner SOUTHWEST UNIVERSITY
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