A quantum dot fluorescent probe and its application in detecting glucose in plant cells
A fluorescent probe, plant cell technology, applied in fluorescence/phosphorescence, luminescent materials, measuring devices, etc., can solve the problem of not being able to detect glucose, etc., and achieve the effect of high accuracy
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[0034] The preparation method of the quantum dot fluorescent probe used in the present invention is as follows: preparing TGA-CdTe quantum dots, and dissolving the TGA-CdTe quantum dots in CdCl 2 and Na 2 S 2 O 3 in the mixed aqueous solution, and then mixed with EDC, NHS and APBA to obtain quantum dot fluorescent probes. details as follows:
[0035] (1) Preparation of TGA-CdTe quantum dots
[0036] Specifically, in a 15 mL small beaker, 127.5 mg of Te powder and 80 mg of NaBH were first mixed under magnetic stirring. 4 Dissolve in 5 mL of double-distilled water, stir for 2 hours to make Te powder disappear, cool the reaction product with an ice bath immediately, then centrifuge at 4 °C, 5000 r / min for 10 min, take the supernatant to obtain 0.2 mmol / L NaHTe aqueous solution.
[0037] Another 91.3mg CdCl 2 ·5H 2 O was dissolved in 100 mL of water, 44 μl of TGA (mercaptoacetic acid) was added, stirred with argon, and the pH was adjusted to 10 with 0.5 mol / L NaOH. Then, 2...
Embodiment 1
[0049] In Example 1, the above quantum dot fluorescent probe BA-QDs was used to detect glucose in green algae cells of unicellular algae.
[0050] Specifically, the unicellular algal plant green algae was incubated with 1 mL of 100 nM BA-QDs for 20 min, washed three times with 1×TES (triethylchlorosilane), and then the green algae were made into sheets and observed under a laser confocal microscope. Photographs were taken to record the fluorescence intensity at 0 / 20 / 40 / 60min time points in 3 different places in the green algae cells, and the first relative fluorescence intensity value at 60min was calculated by the software Image J for the fluorescence imaging graph.
[0051] Another green algae was incubated with 1000 μM glucose solution for 60 min, washed with 1×TES for 3 times to remove the glucose on the surface of the green algae; then incubated with 1 mL of 100 nM BA-QDs for 20 min, washed with 1×TES for 3 times, The algae were made into slices and observed under a laser...
Embodiment 2
[0056] This embodiment 2 utilizes the above-mentioned quantum dot fluorescent probe BA-QDs to detect glucose in Arabidopsis thaliana leaf cells, and the detection method is as follows:
[0057] Specifically, after injecting 100 μL of 100 nM BA-QDs into 4-week-old Arabidopsis leaves, the Arabidopsis leaves were made into slices for observation by laser confocal microscopy, and photos were taken to record different time points at 580-610 nm in the cells of the Arabidopsis thaliana leaves. (0 / 5 / 10 / 20 / 30 / 45 / 60min) fluorescence intensity, the fluorescence image captured by the software Image J was used to calculate the relative fluorescence intensity value at each time point in 3 different places in Arabidopsis leaf cells, and get The first relative fluorescence intensity value at 60min.
[0058] Another 4-week-old Arabidopsis was taken, and 100 μL of 1000 μM glucose solution was injected into Arabidopsis leaves. 10 min later, 100 μL of 100 nM BA-QDs were injected into the same po...
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