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Hirschsprung's disease plasma exosome diagnosis marker and application thereof

A technology of ganglion cells and diagnostic markers, applied in the field of plasma exosome diagnostic markers of intestinal agangliocytosis, can solve problems such as unreported

Active Publication Date: 2019-09-20
NANJING CHILDRENS HOSPITAL
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Problems solved by technology

For example, non-coding RNA in plasma exosomes in gastric cancer is considered to be a new type of cancer marker. At present, research on the function of exosomal miRNA is mostly concentrated in the fields of tumors and central nervous system lesions. In birth defects and congenital Early diagnosis of malformations, especially in intestinal aganglionosis, has not been reported

Method used

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  • Hirschsprung's disease plasma exosome diagnosis marker and application thereof
  • Hirschsprung's disease plasma exosome diagnosis marker and application thereof
  • Hirschsprung's disease plasma exosome diagnosis marker and application thereof

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Embodiment Construction

[0080] The present invention will be further elaborated below by various embodiments.

[0081] 1. Research object selection and grouping basis

[0082] From 2014 to 2019, the inventors collected plasma samples from children with intestinal aganglionosis and normal infants without disease from hospitals such as the Affiliated Children's Hospital of Nanjing Medical University (clinical diagnostic criteria see figure 1 , figure 2 ), through sorting out the sample data, 57 healthy controls and 57 patients with intestinal aganglionosis who met the requirements were selected as the experimental subjects for Real-time PCR detection of exosomal miRNA expression. Specific sample classification criteria are as follows:

[0083] Group A: Healthy control group (n=57, 9 people for microarray screening, 16 people for first-stage verification, 32 people for independent population verification):

[0084] 1. Age between 0 and 6 months;

[0085] 2. No digestive system disease;

[0086] 3....

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Abstract

The invention belongs to the technical field of genetic engineering and clinical medicine and relates to a Hirschsprung's disease plasma exosome diagnosis marker, primer group and kit, and application of the plasma exosome diagnosis marker or primer in the preparation of Hirschsprung's disease diagnosis preparations. The plasma exosome diagnosis marker comprises hsa-miR-494-3p, hsa-miR-668-3p, hsa-miR-323a-3p, hsa-miR-605-3p and hsa-miR-5701. The plasma exosome diagnosis marker has excellent specificity and sensitivity for the diagnosis of Hirschsprung's disease, is suitable for the preparation of Hirschsprung's disease diagnosis preparations, helps prevent invasive diagnosis, and is suitable for early screening and diagnosis.

Description

technical field [0001] The invention belongs to the technical fields of genetic engineering and clinical medicine, and in particular relates to a plasma exosome diagnostic marker for intestinal aganglionosis and its application. Background technique [0002] Intestinal aganglionosis is characterized by the absence of ganglion cells in the distal intestinal tube of the digestive tract, and the main clinical manifestations are meconium constipation, vomiting, and abdominal distension. It is one of the most common congenital digestive system diseases. The incidence of the disease is about 1:2000-5000 in live births, and the ratio of male to female is about 1:4. According to the length of the stenosis, intestinal aganglionosis is mainly divided into short-segment type, common type, long-segment type and whole-colon type, among which the common type accounts for about 75% of cases, and the diseased intestine extends from the middle and distal part of the sigmoid colon to the rec...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883C12N15/11
CPCC12Q1/6883C12Q2600/158C12Q2600/178
Inventor 唐维兵吕修睿李红星李杨
Owner NANJING CHILDRENS HOSPITAL
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