Hyaluronic acid skin protection composition and preparation method and application thereof

A hyaluronic acid and skin protection technology, applied in the field of biomedicine, can solve the problems of ineffective removal of active oxygen free radicals, achieve good skin protection effects, inhibit oxidative stress, and improve skin aging symptoms

Active Publication Date: 2019-09-27
BIOREGEN BIOMEDICAL (CHANGZHOU) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] In order to solve the problem in the prior art that hyaluronic acid cannot effectively remove active oxygen free radicals when it is used for skin protection, this application provides a new type of hyaluronic acid skin protection composition, which not only has skin care functions such as hyaluronic acid moisturizing, but also It also has enhanced antioxidant capacity, which can effectively scavenge active oxygen free radicals, so it can more effectively improve skin aging symptoms and better solve skin aging problems

Method used

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  • Hyaluronic acid skin protection composition and preparation method and application thereof
  • Hyaluronic acid skin protection composition and preparation method and application thereof
  • Hyaluronic acid skin protection composition and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1: Antioxidative effect of hyaluronic acid thiolated derivatives with different thiol content in scavenging free radicals

[0049] The 1,1-diphenyl-2-trinitrophenylhydrazine (DPPH) test method was used to evaluate the free radical scavenging performance of the hyaluronic acid mercapto derivatives used in the present invention. DPPH is widely used in the quantitative determination of the antioxidant capacity of biochemical substances; this method is based on the fact that the DPPH free radical has a single electron and has a strong absorption at a wavelength of 517nm, and its alcohol solution is purple; when there is a free radical scavenger At the same time, the absorption gradually disappears due to the pairing with its single electron, and its fading degree is quantitatively related to the number of electrons it accepts, so a spectrophotometer can be used for rapid quantitative analysis (Sharma et al., Food Chemistry 2009, 113: 1202-1205).

[0050] Hyaluronic ...

Embodiment 2

[0057] Example 2: Antioxidative effect of hyaluronic acid mercapto derivatives with different molecular weights in scavenging free radicals

[0058] Hyaluronic acid thiol derivatives are prepared from sodium hyaluronate of 180KDa, 300KDa and 1,500KDa respectively by the method reported by Shu et al. 38 μmol / g, 33 μmol / g and 40 μmol / g.

[0059] The sodium hyaluronate raw material and the above-mentioned sodium hyaluronate mercapto derivatives were respectively dissolved in a phosphate buffer solvent (pH7.4) to obtain a test sample solution as described in the following table.

[0060]

[0061] According to the DPPH method described in Example 1, the antioxidant effect of the above sample solution in scavenging free radicals was measured.

[0062] The results of this example can be found in figure 2 . Test samples 1, 3 and 5 are sodium hyaluronate control solutions of different molecular weights, and the DPPH free radical scavenging rate is low; Test samples 2, 4 and 6 ar...

Embodiment 3

[0063] Embodiment 3: Preparation of hyaluronic acid skin protection composition (containing sodium hyaluronate of a molecular weight)

[0064] The thiolated derivatives of hyaluronic acid used were prepared from 180KDa sodium hyaluronate using the method reported by Shu et al. , 38 μmol / g, 57 μmol / g, 108 μmol / g, 266 μmol / g, 416 μmol / g and 530 μmol / g.

[0065] The molecular weight of sodium hyaluronate used is 1.6KDa, 3KDa, 8KDa, 50KDa, 180KDa, 500KDa, 800KDa, 1,500KDa, 2,700KDa.

[0066] Any of the above-mentioned hyaluronic acid thiolated derivatives with a thiol content was dissolved in distilled water to obtain a 6 mg / mL solution; any of the above-mentioned sodium hyaluronate with a molecular weight was dissolved in distilled water to obtain a 12 mg / mL solution; then according to 0.9: The volume ratio of 0.1-0.1:0.9 is evenly mixed to obtain hyaluronic acid skin protection compositions with different proportions. In these compositions, the content of hyaluronic acid merca...

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Abstract

The invention discloses a hyaluronic acid skin protection composition. The hyaluronic acid skin protection composition comprises: 1) a sulfhydrylated derivative of hyaluronic acid; and 2) at least one molecular weight of hyaluronic acid or salt thereof. The composition has unique advantages when used for skin protection, not only has multiple skin protection effects of the hyaluronic acid, but also has strong antioxidant capacity, and can effectively scavenge active oxygen radicals.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a hyaluronic acid skin protection composition, and also to a preparation method and application of the hyaluronic acid skin protection composition. Background technique [0002] In the normal metabolic process of the human body, there is a dynamic balance between the oxidation process and the reduction process. However, under the action of a variety of endogenous (such as chronic or acute infection, etc.) Active oxygen free radicals; active oxygen free radicals have strong oxidative properties, can attack lipids, proteins and DNA, etc., can damage the tissues and cells of the body, and then cause various chronic diseases and aging effects (Gutteridge et al., British Medical Bulletin 1999, 55:49-57). [0003] The skin is the largest organ in the human body. Studies have shown that up to 80% of skin aging is related to active oxygen free radicals. Due to the destructive effect of acti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/73A61Q19/08A61Q19/00
CPCA61K8/735A61Q19/08A61Q19/00A61Q19/10
Inventor 王云云王昕宇宋文俊张红晨王坤舒晓正
Owner BIOREGEN BIOMEDICAL (CHANGZHOU) CO LTD
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