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Method for producing mannanase by lactobacillus brevis

A mannanase, Lactobacillus brevis technology, applied in the directions of microorganism-based methods, glycosylases, biochemical equipment and methods, etc., can solve the problem of lack of Lactobacillus brevis and other problems, and achieve the promotion of health care products and feed production. , Easy to operate, high enzymatic activity

Active Publication Date: 2019-10-01
HEILONGJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Lactobacillus plantarum is a Gram-positive bacterium that can utilize sugars to produce lactic acid, but there is no report of Lactobacillus plantarum producing mannanase

Method used

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  • Method for producing mannanase by lactobacillus brevis
  • Method for producing mannanase by lactobacillus brevis

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specific Embodiment approach 1

[0009] Specific embodiment one: the method for present embodiment Lactobacillus brevis to produce mannanase is carried out according to the following steps: one, Lactobacillus brevis (Lactobacillus brevis) HDRS8 is inoculated in polysaccharide carbon source konjac flour culture medium, at a temperature of Cultivate at 28-36°C for 45-55 hours to obtain a fermented liquid; 2. centrifuge the fermented liquid obtained in step 1, discard the precipitate and leave the supernatant to obtain a crude enzyme liquid containing mannanase.

[0010] The Lactobacillus brevis HDRS8 described in this embodiment (from "Study on Isolation, Identification and Culture Conditions of Bacteriocin-Producing Lactic Acid Bacteria") is a chemoheterotrophic lactic acid bacterium, which is isolated from sauerkraut fermentation broth.

[0011] Centrifugation conditions in Step 2 of this embodiment: centrifuge for 15-20 min at a rotational speed of 6000-8000 r / min.

[0012] The activity of mannanase in the c...

specific Embodiment approach 2

[0014] Specific embodiment two: the difference between this embodiment and specific embodiment one is: in step one, the bacterial liquid concentration of Lactobacillus brevis (Lactobacillus brevis) HDRS8 in the polysaccharide carbon source medium is 2.8 × 10 6 ~4.2×10 6 individual / mL. Other steps and parameters are the same as those in the first embodiment.

specific Embodiment approach 3

[0015] Specific embodiment three: the difference between this embodiment and specific embodiment one or two is: in step 1, the bacterial liquid concentration of Lactobacillus brevis (Lactobacillus brevis) HDRS8 in the polysaccharide carbon source medium is 2.8 * 10 6 ~4.2×10 6 individual / mL. Other steps and parameters are the same as those in Embodiment 1 or 2.

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Abstract

The invention discloses a method for producing mannanase by lactobacillus brevis and relates to a method for producing mannanase. The method for producing the mannanase by lactobacillus brevis comprises the specific steps: inoculating lactobacillus brevis HDRS8 into a polysaccharide carbon source culture medium for culture to obtain a fermentation liquor; and then centrifuging the fermentation liquor, discarding the precipitate and leaving the supernatant to obtain a crude enzyme solution containing mannanase. The activity of the mannanase in the obtained crude enzyme solution containing the mannanase and obtained by the method is 45-65 U / mL, the preparation period of the method is short, and the enzyme activity of the mannanase is high.

Description

technical field [0001] The present invention relates to a method for producing mannanase. Background technique [0002] Mannanase can specifically hydrolyze β-1,4 glycosidic bonds, and widely exists in nature, such as higher animals, plants and microorganisms. Microbial mannanase has a wide range of sources, mild action and broad substrates. However, due to the limitation of biological safety, microbial mannanase has limited its application in the fields of food, health products and feed production. There are many members of lactic acid bacteria and high biological safety. Most of the members are probiotics themselves. Excavate the rich resources of lactic acid bacteria in nature to find new strains with the ability to produce mannanase, in order to obtain mannanase production with high biological safety. Bacteria, for the mixed application of production strains and metabolites, can simplify the purification process of enzymes, obtain greater industrial benefits at a lower...

Claims

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Application Information

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IPC IPC(8): C12N9/42C12R1/08
CPCC12N9/2494C12Y302/01078Y02P60/87
Inventor 赵丹王瑶那金葛菁萍
Owner HEILONGJIANG UNIV
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