Method for producing mannanase by lactobacillus brevis

A mannanase, Lactobacillus brevis technology, applied in the directions of microorganism-based methods, glycosylases, biochemical equipment and methods, etc., can solve the problem of lack of Lactobacillus brevis and other problems, and achieve the promotion of health care products and feed production. , Easy to operate, high enzymatic activity

Active Publication Date: 2019-10-01
HEILONGJIANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Lactobacillus plantarum is a Gram-positive bacterium that can utilize sugars to produ

Method used

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  • Method for producing mannanase by lactobacillus brevis
  • Method for producing mannanase by lactobacillus brevis

Examples

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Example Embodiment

[0009] Specific embodiment 1: The method for producing mannanase by Lactobacillus brevis in this embodiment is carried out according to the following steps: 1. Inoculate Lactobacillus brevis HDRS8 in a polysaccharide carbon source konjac flour medium at a temperature of Cultivate for 45-55 hours at 28-36°C to obtain fermentation broth; 2. Centrifuge the fermentation broth obtained in step 1, discard the precipitate and leave the supernatant to obtain a crude enzyme solution containing mannanase.

[0010] The Lactobacillus brevis HDRS8 (from "Isolation and Identification of Bacteriocin-Producing Lactic Acid Bacteria and Research on Culture Conditions") described in this embodiment is a chemoheterotrophic lactic acid bacteria isolated from sauerkraut fermentation broth.

[0011] Centrifugation conditions in step 2 of this embodiment: centrifugation for 15-20 min at a rotation speed of 6000-8000 r / min.

[0012] The mannanase activity in the mannanase-containing crude enzyme solution pre...

Example Embodiment

[0014] Specific embodiment two: this embodiment is different from specific embodiment one in that in step one, the concentration of Lactobacillus brevis HDRS8 in the polysaccharide carbon source medium is 2.8×10 6 ~4.2×10 6 Pieces / mL. The other steps and parameters are the same as in the first embodiment.

Example Embodiment

[0015] Specific embodiment three: This embodiment is different from specific embodiment one or two in that in step one, the concentration of Lactobacillus brevis HDRS8 in the polysaccharide carbon source medium is 2.8×10 6 ~4.2×10 6 Pieces / mL. The other steps and parameters are the same as in the first or second embodiment.

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Abstract

The invention discloses a method for producing mannanase by lactobacillus brevis and relates to a method for producing mannanase. The method for producing the mannanase by lactobacillus brevis comprises the specific steps: inoculating lactobacillus brevis HDRS8 into a polysaccharide carbon source culture medium for culture to obtain a fermentation liquor; and then centrifuging the fermentation liquor, discarding the precipitate and leaving the supernatant to obtain a crude enzyme solution containing mannanase. The activity of the mannanase in the obtained crude enzyme solution containing the mannanase and obtained by the method is 45-65 U/mL, the preparation period of the method is short, and the enzyme activity of the mannanase is high.

Description

technical field [0001] The present invention relates to a method for producing mannanase. Background technique [0002] Mannanase can specifically hydrolyze β-1,4 glycosidic bonds, and widely exists in nature, such as higher animals, plants and microorganisms. Microbial mannanase has a wide range of sources, mild action and broad substrates. However, due to the limitation of biological safety, microbial mannanase has limited its application in the fields of food, health products and feed production. There are many members of lactic acid bacteria and high biological safety. Most of the members are probiotics themselves. Excavate the rich resources of lactic acid bacteria in nature to find new strains with the ability to produce mannanase, in order to obtain mannanase production with high biological safety. Bacteria, for the mixed application of production strains and metabolites, can simplify the purification process of enzymes, obtain greater industrial benefits at a lower...

Claims

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Application Information

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IPC IPC(8): C12N9/42C12R1/08
CPCC12N9/2494C12Y302/01078Y02P60/87
Inventor 赵丹王瑶那金葛菁萍
Owner HEILONGJIANG UNIV
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