Method for detecting antibacterial property of coated antibacterial glass by ATP bio-fluorescence lgCA-lgIA standard curve method

Abstract

The invention relates to a method for detecting antibacterial property of coated antibacterial glass, and the detection steps include: sample preparation and pretreatment; equipment selection, and reagent and medium preparation; species preservation, activation and preparation of bacterial suspension; ATP concentration logarithmic value lgCA-relative fluorescence intensity logarithm value lgIA standard establishment and inoculated bacteria liquid live bacteria ATP concentration CA calibration; sample inoculation, culture and elution recovery; IA determination of recovered liquid and live bacteria ATP concentration CA and TA calculation; antibacterial rate R or antibacterial activity value A calculation; and results evaluation. The method is characterized in that the accurate quantitative test is carried out on the antibacterial property of the glass characterized by R or A by using an ATP fluorometer. The method prescribes that of a control sample and an antibacterial sample after contact for 0 h and culture for 24 h are subjected to elution and recovery, the recovery liquid IA is determined and R or A is characterized and calculated by lgIA; and the result evaluate basis is provided. The method for detecting antibacterial property of antibacterial glass developed by the invention supports the improvement of product quality and market order by an advanced detection technology.

Description

technical field [0001] The invention relates to an antibacterial performance testing method of antibacterial glass, specifically an ATP bioluminescent IgC that uses an ATP fluorescence photometer to accurately and quantitatively detect the antibacterial performance of antibacterial glass characterized by antibacterial rate R or antibacterial activity value A A -lgI A The standard curve method belongs to the technical field of glass antibacterial function detection. Background technique [0002] As a new type of ecological functional material, antibacterial glass adopts nano "dispersion" and "modification" technology to combine antibacterial agent with glass in a chemical bonding way, which belongs to the highest level of coated glass; it is used in medical care, food preservation, transportation and 3C electronic touch products and other fields are widely used. According to the global market observation report, the antibacterial glass market size in 2017 was 180 million US ...

AI Technical Summary

Problems solved by technology

In general, the existing antibacterial performance detection methods have the following common problems in technical content and practical application: First, the experimental strains involved are too small to meet the needs of enterprises for new product development and quality control that keep pace with the times; The experimental process is cumbersome, and the relevant operations are affected by the professional experience of the experimenter, the test error is large, and there is no comparability; the third is that the test cycle is between 48 hours and 72 hours, and the time and economic costs are high

At present, the research on performance testing technology of antibacterial materials in foreign countries is developing towards quantification, rapidity and simplification, focusing on the accuracy and comparability of test results; the principle of ATP fluorescence analysis has been used to formulate ISO20743:2007-2013 Textiles-Determination of antibacterial activity of textile products (Textiles - Determination of antibacterial performance of antibacterial finishing textiles)" and ISO13629-1:2012 "Textiles-Determination of antifungal activity of textile products. The change of ATP content characterizes the anti-microbial / fungal performance of the fluorescence assay, but it is only applicable to textile materials or microporous materials with water absorption and the control sample / mycotic growth value > 0. The key technical aspects are not applicable to glass, ceramics and other inorganic non-metallic materials with non-absorbent hard surfaces and the bacterial growth value of the control sample is <0; at the same time, no evaluation of measurement uncertainty is provided

In addition, the relevant antibacterial performance characterization parameters of this standard method are relatively single, only involving the antibacterial activity value A, while the antibacterial rate R commonly used in my country

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