Aflatoxin detection method based on magnetic noble metal composite nanoparticles and microfluidics
A composite nanoparticle and aflatoxin technology, which is applied in the field of aflatoxin detection, can solve the problems of fast and accurate detection of aflatoxin, poor detection sensitivity, and complicated electrode assembly, so as to shorten the detection time and improve the detection sensitivity. High, easy to find effect
Inactive Publication Date: 2019-10-08
WUHAN UNIV OF TECH
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Problems solved by technology
Thin-layer chromatography is currently the national standard method for the detection of toxins in my country. Although it is easy to operate and low in cost, it is difficult to meet the needs of low detection limits due to its semi-quantitative method and high sensitivity. , good reproducibility, but at the same time there are shortcomings such as time-consuming and complicated operation, which cannot meet the needs of rapid and accurate detection of aflatoxin on-site; The aflatoxin M1 electrochemical assay method of aflatoxin M1 nucleic acid aptamer has high selectivity and detection sensitivity, but the electrode assembly is relatively complicated and requires professionals to complete, and there are weak probe signals, poor detection sensitivity, Disadvantages such as high false positives
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Embodiment Construction
[0022] Aflatoxin detection method based on magnetic noble metal composite nanoparticles and microfluidics:
[0023] The first step, the preparation of monodisperse superparamagnetic Fe 3 o 4 Nanoparticle solution. Using iron acetylacetonate as precursor, 1,2-hexadecanediol as reducing agent, oleic acid and oleylamine as surfactant, monodisperse superparamagnetic Fe with adjustable particle size was synthesized by pyrolysis at high temperature 3 o 4 nanoparticles;
[0024] The second step is to synthesize the magnetic noble metal composite nanoparticle solution, and the superparamagnetic Fe prepared in the previous step 3 o 4 Nanoparticles are the seed core, and Au or Ag or Cu noble metal nanoparticle clusters are deposited by atomic evaporation, magnetron sputtering or pulsed laser or by noble metal ions Au + or Ag + or Cu 2+ self-assembled growth or utilizing electrochemical deposition methods to grow a certain thickness of Au or Ag or Cu noble metal nanoparticle clad...
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Abstract
The invention relates to an aflatoxin detection method based on magnetic noble metal composite nanoparticles and microfluidics. The method mainly comprises the following steps: preparing a synthetic magnetic noble metal composite nanoparticle solution, marking an aflatoxin antibody by using rhodamine 6G, and then using a bifunctional polyethylene glycol modifier with one end connected with a sulfydryl group and the other end provided with an NHS group to be fixed on the surfaces of the magnetic noble metal composite nanoparticles; adding an aflatoxin antigen serum sample with a known concentration, washing for 2 -3 times, gathering the formed immune complexes at a point in a micro-channel by using a magnet, irradiating the point with a portable Raman spectrometer, reading data, drawing a standard curve, establishing a standard model after analyzing the data, and finally, diluting a small amount of the sample into a mixed colloidal solution, measuring a Raman spectrum signal, and determining the concentration of the aflatoxin antigen in the sample according to the standard model. The method is simple and convenient to operate, the to-be-detected object is fully enriched, amplification of the detection signal is facilitated, the detection precision is improved, and the detection time is shortened.
Description
technical field [0001] The invention relates to the field of food safety detection, in particular to a detection method for aflatoxin based on noble metal nanoparticles. Background technique [0002] Food safety is closely related to people's production and life as well as economic and social development. Food safety issues have attracted more and more attention from the country and the people. Among them, mycotoxins are an important factor leading to food safety problems. The current carcinogenic mycotoxins mainly include yellow Aspergillus, fumonisins, hertoxins, zearalenone and variegated aspergillus, among which aflatoxins are the most toxic and carcinogenic mycotoxins in contaminated agricultural products. At present, the detection methods for aflatoxins at home and abroad are mainly based on physical, chemical and immunological analysis methods, mainly including thin-layer chromatography, high performance liquid chromatography, mass spectrometry and capillary electroph...
Claims
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IPC IPC(8): G01N33/543G01N33/532
CPCG01N33/54346G01N33/5434G01N33/532G01N2333/38
Inventor 吉晓莉吴思佳张净铭郑伟鹏雷一凡冯凌志饶新楠
Owner WUHAN UNIV OF TECH