Human herpesvirus I/II/III/V type nucleic acid typing detection kit and detection method

Abstract

The invention discloses a human herpesvirus I / II / III / V type nucleic acid typing detection kit. The kit comprises a rapid virus nucleic acid extraction reagent, a PCR amplification reagent, a human herpesvirus I / II / III / V type nucleic acid detection reagent, a positive reference substance and a negative reference substance. According to the kit, multiple channel primer probes with human herpesvirusI / II / III / V type specificity are introduced so that the workload can be decreased, the problem is also avoided that other detection methods are not high in specificity and easily cause missed diagnosisand misdiagnosis, and it can be achieved that the same system can multiply and simultaneously detect four pathogens without mutual interference of the primer probes of all channels. The invention notonly discloses a real-time fluorescence quantitative PCR method aiming at human herpesvirus I / II / III / V type typing detection for the first time, but also has the advantages of good sensitivity, highspecificity, accuracy, reliability, rapidness, convenience and the like, thereby realizing rapid diagnosis of the infection situation of a herpesvirus I / II / III / V type. The invention also provides a human herpesvirus I / II / III / V type nucleic acid typing detection method.

Description

technical field [0001] The invention belongs to the technical field of in vitro diagnostic reagents, in particular to a human herpesvirus type I / II / III / V nucleic acid typing detection kit and detection method. Background technique [0002] Herpesviruses are a class of enveloped DNA viruses that primarily invade tissues of ectodermal origin, including skin, mucous membranes, and nervous tissue. The site of infection and the diseases it causes are various, and there is a tendency of latent infection, which seriously threatens human health. Currently known human herpes viruses include eight types, namely, herpes simplex virus type 1 (human herpes virus type I), herpes simplex virus type 2 (human herpes virus type II), varicella-zoster virus (human herpes virus type III) Type), Epstein-Barr virus (human herpesvirus type IV), cytomegalovirus (human herpesvirus type V), human herpesvirus type VI, human herpesvirus type VII, human herpesvirus type VIII. [0003] Herpes simplex (h...

AI Technical Summary

Problems solved by technology

[0009] (3) 12 Encephalitis Virus Nucleic Acid Multiplex PCR Detection Kits and Their Application (CN201510886216.7), including the detection of human herpesvirus I / II / III / V, the detection method is capillary electrophoresis, and the operation is relatively cumbersome and difficult to detect The time is long, the detection sensitivity is lower than that of fluorescent quantitative PCR method, and it is easy to produce false positives

[0010] However, the four-fold detection method for human herpesvirus I / II / III / V nucleic acid typing has not been reported on the fluorescent quantitative PCR technology platform. Therefore, it is necessary to use this platform to develop a highly sensitive, highly specific, and highly accurate A unique multiplex real-time fluorescent quantitative PCR detection method and kit to realize quadruple combined detection and distinguish human herpesvirus types Ⅰ / Ⅱ / Ⅲ / Ⅴ

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