Preparation method and application of aptamer electrochemical sensor

An aptamer and electrochemical technology, which is applied in the field of biosensors and electrochemical detection, can solve the problems of high maintenance costs, unsatisfactory rapid detection, expensive instruments and equipment, etc., achieve low cost, improve electrode surface state, and sample consumption little effect

Inactive Publication Date: 2019-10-11
SHANXI UNIV
View PDF3 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, most of the detection methods of bisphenol A are traditional detection methods based on large-scale instruments, which have the disadvantages of expensive equipment, high maintenance costs, and the need for professional and technical personnel to operate, which are increasingly unable to meet the needs of today's rapid food safety detection.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method and application of aptamer electrochemical sensor
  • Preparation method and application of aptamer electrochemical sensor
  • Preparation method and application of aptamer electrochemical sensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Based on SPCE, graphene and nano gold, the construction process of the aptamer electrochemical sensor for the detection of BPA is as follows: figure 1 Shown:

[0029](1) Activation of SPCE: Draw 80 µL of 0.1 mol / L sulfuric acid solution and drop it on the surface of the electrode, and activate it by cyclic voltammetry (CV). 20 laps. Finally, the cyclic voltammetry test was carried out in 10 mmol / L potassium ferricyanide solution, the scanning range was -0.4~0.8 V, and the scanning speed was 100 mV / s, and the peak potential difference of the obtained cyclic voltammetry curve was observed (200 mV about). Then rinse with absolute ethanol and ultrapure water in sequence, and finally blow dry with nitrogen to obtain the activated SPCE.

[0030] (2) Preparation of GR / SPCE: PEDOT:PSS was mixed with ultrapure water at a ratio of 1:1 as a graphene dispersant, and a dispersion liquid with a graphene concentration of 0.25 mg / mL was prepared. Pipette 5 µL of the above on the su...

Embodiment 2

[0034] Construction of an aptamer electrochemical sensor for the detection of BPA based on SPCE, graphene and gold nanoparticles:

[0035] (1) Activation of SPCE: 90 µL of 0.1 mol / L sulfuric acid solution was dropped on the surface of the electrode, activated by cyclic voltammetry (CV), the scanning range was -0.6~1.5 V, and the scanning speed was 100 mV / s. 30 laps. Finally, the cyclic voltammetry test was carried out in 10 mmol / L potassium ferricyanide solution, the scanning range was -0.4~0.8 V, and the scanning speed was 100 mV / s, and the peak potential difference of the obtained cyclic voltammetry curve was observed (200 mV about). Then rinse with absolute ethanol and ultrapure water in sequence, and finally blow dry with nitrogen to obtain the activated SPCE.

[0036] (2) Preparation of GR / SPCE: PEDOT:PSS was mixed with ultrapure water at a ratio of 1:1 as a graphene dispersant, and a dispersion liquid with a graphene concentration of 0.5 mg / mL was prepared. Pipette 8 ...

Embodiment 3

[0040] Construction of an aptamer electrochemical sensor for the detection of BPA based on SPCE, graphene and gold nanoparticles:

[0041] (1) Activation of SPCE: 85 µL of 0.1 mol / L sulfuric acid solution was dropped on the surface of the electrode, activated by cyclic voltammetry (CV), the scanning range was -0.6~1.5 V, and the scanning speed was 80 mV / s. 25 laps. Finally, the cyclic voltammetry test was carried out in 10 mmol / L potassium ferricyanide solution, the scanning range was -0.4~0.8 V, and the scanning speed was 100 mV / s, and the peak potential difference of the obtained cyclic voltammetry curve was observed (200 mV about). Then rinse with absolute ethanol and ultrapure water in sequence, and finally blow dry with nitrogen to obtain the activated SPCE.

[0042] (2) Preparation of GR / SPCE: PEDOT:PSS was mixed with ultrapure water at 1:1 as a graphene dispersant, and a dispersion liquid with a graphene concentration of 0.35 mg / mL was prepared. Pipette 7 µL of the a...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
concentrationaaaaaaaaaa
Login to view more

Abstract

The invention discloses a preparation method and application of an aptamer electrochemical sensor. The preparation method comprises the following steps: firstly, activating a screen-printed carbon electrode (SPCE), then dispersing graphene with a solution prepared from poly(3,4-ethylenedioxythiophene)-poly(styrenesulfonate) (PEDOT:PSS) and ultrapure water in a volume ratio of 1:1, modifying the surface of the SPCE with graphene dispersion, and drying under an infrared lamp to obtain a graphene-modified SPCE (GR/SPCE); secondly, preparing a nanogold-modified GR/SPCE (AuNPs/GR/SPCE) with an electrodeposition method; and finally modifying the surface of the electrode with an aptamer of which one end is modified with sulfydryl in an Au-S bond covalent binding manner to obtain an aptamer-modified AuNPs/GR/SPCE (Apt/AuNPs/GR/SPCE). According to the method, the surface of a disposable SPCE is modified with the graphene, nanogold and a bisphenol A aptamer to build an aptamer electrochemical sensor amplified on the basis of graphene and nanogold signals. The aptamer electrochemical sensor can detect bisphenol A rapidly, sensitively, efficiently and conveniently at low cost, and has relatively good practical application value.

Description

technical field [0001] The invention relates to a preparation method and application of an aptamer electrochemical sensor, in particular to a preparation method and application of an aptamer electrochemical sensor based on screen-printed carbon electrodes, graphene and nano gold, belonging to biosensors and Electrochemical detection technology field. Background technique [0002] Bisphenol A (BPA) is an endocrine disruptor that has great potential harm to the human reproductive system, cardiovascular system, and immune system. Studies in recent years have also shown that bisphenol A can induce gene mutations, such as DNA adducts, aneuploidy, and mutagenicity, which can lead to infertility, miscarriage, and birth defects. The main methods currently used to detect BPA include ultra-high performance liquid chromatography-quadrupole string method, molecular imprinted magnetic solid-phase extraction / liquid chromatography, gas chromatography-mass spectrometry, spectrophotometry, ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N27/30G01N27/48
CPCG01N27/308G01N27/3278G01N27/48
Inventor 杨钰昆吕巧郭彩霞王小敏张国华尉立刚张锦华范三红
Owner SHANXI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap