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Bifidobacterium bifidum ccfm1063 and its application

A technology of Bifidobacterium bifidum and functional bacterial agent, applied in the field of microorganisms, can solve the problems of increasing the risk of death of type 2 diabetes, and achieve the effect of alleviating the toxicity of PFOA, alleviating anxiety and depression, and having wide application prospects.

Active Publication Date: 2022-04-29
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, studies have shown that workers exposed to PFOA have an increased risk of death from type 2 diabetes. Therefore, PFOA exposure in daily life is a potential risk of human type 2 diabetes.

Method used

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  • Bifidobacterium bifidum ccfm1063 and its application
  • Bifidobacterium bifidum ccfm1063 and its application
  • Bifidobacterium bifidum ccfm1063 and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0090] Example 1: Bifidobacterium bifidum CCFM1063 has good tolerance to simulated gastrointestinal fluid

[0091] The cryopreserved Bifidobacterium bifidum CCFM1063 was inoculated in mMRS medium (MRS medium + 0.05% cysteine ​​hydrochloride), cultured anaerobically at 37°C for 48 hours, and then subcultured in mMRS medium for 2-2 hours. After 3 times, take 1 mL of the culture solution of Bifidobacterium bifidum CCFM1063, mix it with 9.0 mL of pH 2.5 artificial simulated gastric juice (containing 1% pepsin, mMRS medium at pH=2.5), and culture it anaerobically at 37°C. Samples were taken at 0h, 0.5h, 1h, and 2h, and the mMRS agar medium was used to pour culture to count plate colonies, determine the number of viable bacteria and calculate their survival rate.

[0092] The survival rate is the ratio of the logarithmic value of the number of viable bacteria at the time of sampling to the logarithmic value of the number of viable bacteria at the 0th hour in the culture solution, ex...

Embodiment 2

[0097] Example 2: Bifidobacterium bifidum CCFM1063 has no toxic side effects on C57BL / 6J mice

[0098] Bifidobacterium bifidum CCFM1063 cells were resuspended in 2% sucrose solution to make a concentration of 3.0×10 9CFU / mL bacterial suspension. Eight healthy male C57BL / 6J mice with a body weight of about 16-20 g were taken. After one week of adaptation to the environment, the bacterial suspension of this concentration was administered orally once a day, observed for one week, and the death and body weight were recorded.

[0099] The results of these tests are listed in Table 3. These results indicate that feeding concentrations of 3.0×10 9 CFU / mL of Bifidobacterium bifidum CCFM1063 had no significant effect on mice, no significant change in body weight, and no death. The appearance of the mice had no obvious pathological symptoms.

[0100] Table 3 Changes in body weight and death of mice

[0101]

[0102] Note: -: no death of mice

Embodiment 3

[0103] Example 3: Bifidobacterium bifidum CCFM1063 has a recovery effect on intestinal flora imbalance in type 2 diabetic mice

[0104] Take 48 healthy male C57BL / 6J mice weighing 16-20g, adapt to the environment for 1 week, and randomly divide them into 6 groups: blank control group (NC), model control group (M), rosiglitazone control group (RH) , Bifidobacterium bifidum CCFM1063 intervention group (CCFM1063), Bifidobacterium bifidum CCFM10632 control group (CCFM10632) each group contained 8 mice, and the dose of intragastric bacterium suspension was 3.0×10 9 CFU / mL, resuspended in 3% sucrose solution. The grouping and treatment methods of experimental animals are shown in Table 4:

[0105] Table 4 Grouping of experimental animals

[0106]

[0107]

[0108] Week 2-7: The mice in the normal group were fed with normal feed, and the rest of the mice were fed with high-fat feed.

[0109] On the 1st day of the 11th week, all mice were fasted for 12 hours, the normal group...

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Abstract

The invention discloses Bifidobacterium bifidum CCFM1063 and its application. Bifidobacterium bifidum CCFM1063 can quickly colonize the intestinal tract, significantly improve fasting blood sugar and oral glucose tolerance caused by type 2 diabetes, and reduce the area under the curve of glucose tolerance; Significantly improve the increase of total cholesterol and decrease of high-density lipoprotein cholesterol caused by type 2 diabetes; significantly improve the insulin resistance caused by type 2 diabetes; significantly improve the level of inflammation in liver tissue of type 2 diabetes; significantly improve the level of inflammation caused by type 2 diabetes Bifidobacterium bifidum CCFM1063 has a strong adsorption capacity for perfluorooctanoic acid, and has the ability to alleviate the toxicity of perfluorooctanoic acid; it can significantly improve the constipation caused by type 2 diabetes and increase the level of Allobaculum in the intestine , has the effect of relieving anxiety, depression and colitis.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to Bifidobacterium bifidum CCFM1063 and application thereof. Background technique [0002] In recent years, the development of the economy has led to changes in the lifestyle of the Chinese people, a decrease in activity, a significant increase in the proportion of obesity, and a substantial increase in the prevalence of diabetes and metabolic syndrome (Metabolic Syndrome). According to the International Diabetes Federation (IDF), in 2017, 425 million people over the age of 19 suffered from diabetes worldwide. If this trend continues, the number of diabetic patients will reach 693 million in about 30 years. Therefore, controlling diabetes has become an urgent matter. [0003] Because type 2 diabetes is a pathological state in which a variety of metabolic components are abnormally aggregated, and its clustering is related to insulin resistance, it has become a com...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23L33/135A61K35/745A61P1/00A61P1/10A61P1/16A61P1/18A61P3/10A61P25/22A61P25/24C12R1/01
CPCC12N1/20A23L33/135A61K35/745A61P1/00A61P1/10A61P1/16A61P1/18A61P3/10A61P25/22A61P25/24A23V2002/00C12R2001/01C12N1/205A23V2200/328A23V2200/3262A23V2200/32A23V2200/31A23V2400/517
Inventor 王刚陈卫司倩赵建新张灏
Owner JIANGNAN UNIV
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