Immunochromatographic capillary based on colloidal gold photothermal effect and application thereof

Abstract

The invention provides an immunochromatographic capillary based on the colloidal gold photothermal effect and application thereof. A glass capillary is used as a carrier, the colloidal gold is used asa tracer and a capillary immunochromatographic method for the detection of Salmonella based on the colloidal gold photothermal effect is developed according to the sandwich principle of double antibodies. According to the method, the glass is used as the solid phase carrier and has high temperature resistance and high ionic strength and is more stable and is not liable to break or influenced by the outside world and is cheap and easy to obtain. The colloidal gold is used as the tracer to construct the immunochromatographic capillary, and the visual detection of salmonella in the sample can bequickly realized. Meanwhile, the temperature change value is obtained by laser irradiation by using the excellent photothermal conversion performance of the colloidal gold, and the quantitative and sensitive detection of Salmonella is realized by using the immunochromatographic capillary so as to have good practical application value.

Description

technical field [0001] The invention belongs to the technical field of microbial detection, and in particular relates to an immunochromatographic capillary based on the photothermal effect of colloidal gold and an application thereof. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Salmonella, belonging to the Enterobacteriaceae family, is a facultative anaerobic Gram-negative bacterium. Because Salmonella does not belong to spoilage bacteria, food contamination is difficult to be detected by the naked eye, and food poisoning symptoms are mainly manifested after people eat it, namely vomiting and diarrhea. Therefore, Salmonella is considered to be one of the main causes o...

AI Technical Summary

Problems solved by technology

The traditional detection method needs to go through the steps of pre-enrichment, enrichment, isolation and culture, biochemical tests and serological identification of the isolated and cultured colonies to determine the bacterial type. Although the detection results are accurate and reliable, the above-mentioned method has complicated operation steps and long detection time. Difficult to meet the needs of rapid detection of Salmonella in food

Rapid detection methods include fluorescent immunoassay, enzyme-linked immunosorbent assay, and immunochromatography, among which, immunochromatography has made great progress in recent years, but the inventors have found that the solid-phase carrier of the current immunochromatography Nitrocellulose membrane is mainly used, its production process is complicated, and the sampling environment can easily affect the combination of the sample and the membrane, and it is also fragile and easy to break, which is easy to cause uneven color development and dragging in the results.

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