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Simple method for extracting and purifying ferulic acid from green wheat kernel bran

A technology of green wheat kernel bran and ferulic acid, which is applied in the field of simple extraction and purification of ferulic acid from green wheat kernel bran, can solve the problems of cumbersome operation, high flow rate, and loss of target substances, so as to improve extraction efficiency, Environmentally friendly and easy to operate

Inactive Publication Date: 2019-10-25
HENAN UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But this method obtains the mixture of cis and trans ferulic acid, and the reaction time is long, the efficiency is low and the yield is low; the ferulic acid obtained from plants is mainly obtained by acid method, alkali method and enzymatic method and three methods. ferulic acid preparation
Due to the dense structure of lignin, the hydrolysis efficiency of enzymatic hydrolysis is low
Although the alkaline method can break the ester bond and release ferulic acid, it is difficult to separate ferulic acid because polysaccharides, lignin and its monomers enter the lye at the same time, and most of the existing purification methods use macroporous resin adsorption. , the operation is cumbersome, and the loss of the target is serious; the preparative liquid chromatography also has certain limitations, such as the flow rate is too high, the retention time of the target and impurities is too close, affecting the collection rate and purity

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] After the raw materials are deproteinized, starch and pigment processed in advance, the deproteinized, starch, and pigmented green wheat kernel bran is crushed to 30 meshes, and 0.2g of the processed green wheat kernel bran and 0.8 sodium hydroxide are weighed. g. Put 10ml of distilled water in a test tube with a stopper and soak for 12h at room temperature for extraction (18:00-6:00 next day). Afterwards, the centrifugal liquid was centrifuged at 3500 r / min for 10 min, and then washed with distilled water and centrifuged 3 times. After adjusting the pH of the collected centrifugal liquid to 3, the acidic solution was suction filtered and repeated 3 times; the acidified centrifugal liquid was extracted with ethyl acetate at a volume ratio of 1:1, and the ethyl acetate layer was collected after repeated 3 times to obtain a clear The pale yellow solution. When the cold hydrazine temperature of the rotary evaporator drops below minus 10℃, the speed is adjusted to 55r / min, ...

Embodiment 2

[0026] After the raw materials are deproteinized, starch and pigment processed in advance, the deproteinized, starch, and pigmented raw green wheat kernel bran is crushed to 50 mesh, and the processed green wheat kernel bran 1.0 g and sodium hydroxide 4.0 are weighed. g. Place 50ml of distilled water in a test tube with a stopper, and soak for 12h at room temperature for extraction (18:00-6:00 next day). Afterwards, the centrifugal liquid was centrifuged for 8 minutes under the centrifugal condition of 3000r / min, and the centrifugation was repeated 3 times by washing with distilled water. After adjusting the pH of the collected centrifugal liquid to 2, the acidic solution was suction filtered and repeated 3 times; the acidified centrifugal liquid was extracted with ethyl acetate at a volume ratio of 1:1, and the ethyl acetate layer was collected after repeated 3 times to obtain a clear The pale yellow solution. When the cold hydrazine temperature of the rotary evaporator drops...

Embodiment 3

[0028] After the raw materials are deproteinized, starch and pigment processed in advance, the deproteinized, starch, and pigmented raw green wheat kernel bran is crushed to 40 meshes, and 5.0 g of the processed green wheat kernel bran and sodium hydroxide 25.0 are weighed. g. Put 250ml of distilled water in a test tube with a stopper, soak for 12h at room temperature for extraction (18:00-6:00 next day). After that, the centrifugal liquid was centrifuged at 3200r / min for 15min, and then washed with distilled water and centrifuged 3 times. After adjusting the pH of the collected centrifugal liquid to 4, the acidic solution was suction filtered and repeated 3 times; the acidified centrifugal liquid was extracted with ethyl acetate at a volume ratio of 1:1, and the ethyl acetate layer was collected after repeated 3 times to obtain a clarification. The pale yellow solution. When the cold hydrazine temperature of the rotary evaporator drops below minus 10℃, the speed is adjusted t...

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Abstract

The invention discloses a simple method for extracting and purifying ferulic acid from green wheat kernel bran. The simple method includes the steps that the raw green wheat kernel bran is crushed, and then soaking at the room temperature and extraction are conducted by using a certain ratio of material to liquid (g / ml) and the mass fraction of lye; then after a water extract is centrifuged, pH isadjusted to acidic and suction filtration is conducted, then extraction is repeated with ethyl acetate, and an organic phase is collected and rotary evaporate is conducted to dryness to recover ethylacetate; and after boiling distilled water is dissolved in the ferulic acid in dry matter, crystallisation and collection are conducted after refrigeration and drying to achieve the purposes of separation and purification and obtainment of high purity ferulic acid.

Description

Technical field [0001] The invention belongs to the field of separating extracts from plants, and particularly relates to a simple method for extracting and purifying ferulic acid from green wheat kernel bran. Background technique [0002] Green wheat kernels are wheat grains in late milk and wax ripening stages. Green wheat kernels are rich in protein, dietary fiber and α and β amylases. [1] It also contains active substances such as phenolic acids and flavonoids, which are mainly found in the bran. Among them, ferulic acid is one of the phenolic acids. [0003] The chemical name of ferulic acid is 4-hydroxy-3-methoxycinnamic acid ( Ferulic acid, FA ), is one of the derivatives of cinnamic acid, has strong anti-oxidation and reducibility, easy to decompose when exposed to light [2] . Ferulic acid is a biologically active substance with many unique functions. It is both an antioxidant and an anti-inflammatory factor. It can scavenge excess ROS to prevent oxidative damage, or direc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C51/42C07C59/64
CPCC07C51/42C07C59/64
Inventor 张国治杨徐宁张富重许仕文许柠
Owner HENAN UNIVERSITY OF TECHNOLOGY
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