Saccharomyces cerevisiae and its uses
A Saccharomyces cerevisiae, application technology, applied in the direction of fermentation, fungi, microorganisms, etc., can solve the problems of low squalene content and high production cost
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Embodiment 1
[0035] Example 1 Determination of Separation of Yeast in Alcohol
[0036] Sample Collection: In December 2015, the farmer alcohol was purchased on the country market in Hunan Yongzhou, Shandong Yucheng, Yunnan Yuming and Guangdong milk source. It took back to the laboratory refrigerator 4 ° C temporarily.
[0037] 1) Separation and purification: 1 g of the above-mentioned four alcoholic saline, mixed into 10 ml of sterile saline, mix, separated from the YPD plate, stationary culture for 48 h at 30 ° C, select the smooth and viscous surface of the surface It is easy to provoke, uniform colony, a single colonus which is uniform, and the front and edges are uniform, respectively, and the secondary scribe of YPD plate is separated, respectively, at 30 ° C for 48 h. Picking the same flat plate with the SLS, the mirror examination is initially confirmed as yeast, selecting a good single bacterial laundry transmissory, part of which is used for the preservation of strains, partially used...
Embodiment 2
[0044] Example 2 identification of strains
[0045] The separated strain of the separated strain preserved in Example 1 further morphological identification and molecular biological identification, the steps are as follows:
[0046] Identification of appearance: The preserved X8 strain is separated from the YPD plate medium overline, and the colonies are observed as spherical projections, smooth surface smooth, opaque, creamy; have wine fragrance; further colonies To take a small number of sterile water droplets on the slide, then take a small amount of bacteria and mixed with sterile water, plus the cover slide, directly observe, can be seen under the oil mirror, 5-10 μm in diameter, in diameter, part Typical yeast sprouts. Specific form figure 1 Combined with the above appearance phenotype and initial screening source, it is possible to initially determine that this strain is a bacterial yeast.
[0047] Molecular Identification: The X8 strain genome is a template, and the fungi ...
Embodiment 3
[0051] Example 3 ARTP mutagenesis screening of brewing yeast
[0052] X8 brewery yeast with the highest yield of strglene is started with strains, and mutagenesis is carried out using atmospheric room temperature plasma mutagenesis system ARTP-M.
[0053] Investigations in mutagenesis conditions were first prepared by preparing X8 strains, then taking 10 μl of coated mutagenesis supporting sheet, coating 16, then ARTP mutagenesis treatment 0S, 10S, 20S, 30S, 40S, 50S, 60S, and 70S, each processing time is two parallel, and then put the mutagenevous processing of the sample sheet in 1 ml of physiological saline oscillation elutch, and finally use aseptic saline to dilute, apply Cloth YPD tablet for colonies count. Statistical lethal life results figure 2 . It is understood that the mortality rate of the strain is about 95% or more, and the treatment time of 50s and or more is 100%, so that the optimal mutagenesis treatment time is 40s. Preparation of the initial X8 strain was prepa...
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