Surface enhanced Raman spectrum detection method combining gel electrophoresis separation technology

A surface-enhanced Raman and electrophoretic separation technology, applied in Raman scattering, material analysis by electromagnetic means, measurement devices, etc., can solve problems such as restricting the development of surface-enhanced Raman spectroscopy and hybrid detection, and achieve a significant enhancement effect. , the detection process is simple, the effect of high detection

Active Publication Date: 2019-10-25
XUZHOU NORMAL UNIVERSITY
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Problems solved by technology

SERS can detect a single substance, but in the face of more complex detection samples, such as multiple mixtures with similar surface-enhanced Raman spectra and molecular physical properties that are diffic

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  • Surface enhanced Raman spectrum detection method combining gel electrophoresis separation technology
  • Surface enhanced Raman spectrum detection method combining gel electrophoresis separation technology
  • Surface enhanced Raman spectrum detection method combining gel electrophoresis separation technology

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Embodiment

[0022] A surface-enhanced Raman spectroscopy detection method combined with gel electrophoresis separation technology, comprising the following steps:

[0023] (1) if figure 1 As shown, cut the glass sheet into 1.5cm×7.5cm, add ethanol and wash it in an ultrasonic cleaner for 5 minutes, repeat three times, then dry it with nitrogen gas, and fix it in the deposition chamber; when the pressure is less than 5×10 -7 In the Torr vacuum deposition chamber, through electron beam evaporation, a layer of titanium thin film and silver thin film are successively deposited on the cleaned glass at the speed of 0.2nm / s and 0.3nm / s, and then the sample stage is rotated to make the substrate normal and the deposition The included angle of the direction is 86°. By electron beam evaporation, a 940nm thick silver nanorod array is grown at a speed of 0.3nm / s. The diameter of the silver nanorod is 92nm, and the interval between two adjacent silver nanorods is 95nm.

[0024] (2) if figure 2 As ...

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Abstract

A surface enhanced Raman spectrum detection method combining a gel electrophoresis separation technology comprises the following steps of a, preparing a silver nanorod array using an inclined angle deposition technology; b. combining a gel electrophoresis technology to combine a substrate prepared in step a and a gel electrophoresis apparatus; c, adjusting a voltage and a current so that a mixtureto be tested is expanded according to different positions on the substrate; and d, using a surface-enhanced Raman spectrograph to detect surface-enhanced Raman spectrograms at the different positionson the substrate so as to distinguish different components in the mixture. By using the detection method, a detection process is simple, sensitivity is high, an enhanced effect is obvious, repeatability is good and large scale production can be performed. Characteristics of high efficiency of gel electrophoresis and high detection performance of silver nanorods can be fully used so as to achieveseparation detection of multiple similar mixtures, and the method can be applied to the separation detection of the multiple similar complex samples.

Description

technical field [0001] The invention relates to a surface-enhanced Raman spectrum detection method, in particular to a surface-enhanced Raman spectrum detection method combined with gel electrophoresis separation technology. Background technique [0002] In recent years, surface-enhanced Raman spectroscopy (surface-enhanced Raman scattering, SERS) amplifies the electromagnetic field by exciting localized surface plasmons, thereby obtaining molecular structure information and sensitive detection limits. SERS is widely considered as a convenient and sensitive analytical tool due to its non-destructive and fingerprint-like resolution ability, weak impact on the water environment, and high sensitivity, and has been applied in many fields such as biological analysis, food safety, and environmental science fields. SERS can detect a single substance, but in the face of more complex detection samples, such as multiple mixtures with similar surface-enhanced Raman spectra and molecul...

Claims

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Application Information

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IPC IPC(8): G01N21/65G01N27/447
CPCG01N21/658G01N27/447
Inventor 韩彩芹宋宇航李静文
Owner XUZHOU NORMAL UNIVERSITY
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