Inorganic nitrogen detection method based on image analysis
A detection method and image analysis technology, applied in the detection field, can solve the problems of expensive specific sensing equipment, large color difference error, inconvenient water body detection, etc., and achieve the effect of low detection cost, good accuracy and easy promotion
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no. 1 example
[0124] The first embodiment: the whole process of detecting the concentration of ammonia nitrogen solution
[0125] The solution to be tested and the ammonia nitrogen standard solution in the concave hole of the white porcelain plate are subjected to color development treatment to obtain the reaction solution to be tested and the standard reaction solution.
[0126] Specifically, 12 groups of samples were prepared, which were 12 bottles of fermentation broth cultured by different strains for 24 hours, and the concentration of ammonia nitrogen in the fermentation broth was quickly determined by a white porcelain plate. Take a 12-hole white porcelain plate, add 150 μL ammonia nitrogen standard solution with an ammonia nitrogen content of 0-98.3 mg / L and 150 μL sample to be tested in sequence. Then add 20 μL metal ion masking agent and 30 μL ammonia nitrogen chromogen to each well, mix well and let stand for 5 minutes.
[0127] Use the gray calibration color card to adjust the w...
no. 2 example
[0155] Second embodiment: the whole process of detecting the concentration of nitrate nitrogen solution
[0156] The solution to be tested and the standard solution of nitrate nitrogen in the concave hole of the white porcelain plate are subjected to color development treatment to obtain the reaction solution to be tested and the standard reaction solution.
[0157] Specifically, prepare the following samples: sample 1 is sodium nitrite solution (nitrite nitrogen content is 25mg / L), sample 2 is nitrate-containing sewage, and sample 3 is sodium nitrite (nitrite Salt nitrogen content is 25mg / L). Take a 12-hole white porcelain plate, add 50 μL nitrate nitrogen standard solution with a nitrate nitrogen content of 0-29.94 mg / L and 50 μL nitrate nitrogen test sample in sequence. Then add 50 μL of nitrite masking agent to each well, let it stand for 1 minute, then add 200 μL of nitrate nitrogen chromogenic reagent, mix well and let stand for 5 minutes.
[0158] Use the gray calibra...
no. 3 example
[0175] The third embodiment: the whole process of detecting the concentration of nitrite nitrogen solution
[0176] The solution to be tested and the standard solution of nitrite nitrogen in the concave hole of the white porcelain plate are subjected to color development treatment to obtain the reaction solution to be tested and the standard reaction solution.
[0177] Specifically, the following samples were prepared: Samples 1-6 were 6 water samples enriched in nitrifying bacteria, and the enrichment of nitrifying bacteria was reflected by detecting the amount of nitrite generated. Take a 12-hole white porcelain plate, add 150 μL of nitrite nitrogen standard solution with a nitrite nitrogen content of 0-12.05 mg / L and 150 μL of the sample to be tested in sequence. Then add 100 μL of nitrite nitrogen chromogen to each well, mix well and let stand for 5 minutes.
[0178] Use the gray calibration color card to adjust the white balance of the acquisition parameters of the mobil...
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