Fermented traditional Chinese medicine preparation and preparation method and application thereof
A traditional Chinese medicine preparation and traditional Chinese medicine technology, applied in the field of animal husbandry, can solve problems affecting the quality and flavor of livestock and poultry products, natural ecological environment pollution, human health hazards, etc., and achieve the effects of reducing breeding risks, breeding safety, and avoiding pollution
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Embodiment 1
[0055] Example 1, Screening of Saccharomyces cerevisiae JM01 CGMCC No.1803
[0056] 1. Isolation, screening and identification of Saccharomyces cerevisiae
[0057] 1. Isolation of Saccharomyces cerevisiae
[0058] Weigh 10g of crushed wine lees, add 90mL of sterile saline to dilute, inoculate on YPD solid medium by plate coating method, incubate at 28°C for 24h-28h, observe and record the colony morphology.
[0059] 2. Saccharomyces cerevisiae screening
[0060] The isolated yeast strain was inoculated into WL agar medium after being inoculated with YPD liquid medium for 24 hours of activation, and observed after culturing at 27°C for 5 days. The color of the screened colonies was cream (light yellow) to green, and the surface was spherical protrusions, smooth, Opaque, creamy strain. Filtered to JM01, JM02, JM03.
[0061] 3. UV mutagenesis and screening
[0062] Pour the sterilized solid YPD medium into a plate with a flat bottom. After solidification, take an appropriate...
Embodiment 2
[0085] Embodiment 2, the screening of bacillus licheniformis (Bacillus Licheniformis) ZYFJ01
[0086] 1. Isolation, screening and identification of Bacillus Licheniformis ZYFJ01
[0087] 1. Isolation and cultivation of bacterial strains
[0088] Aseptically collect the jejunum of 28-day-old healthy AA broiler chicken, scrape the jejunum mucosa of the broiler chicken with a sterilized slide, dilute it with an appropriate amount of sterile saline, inoculate it on the MRS medium by plate coating method, and culture it at 37°C for 48h-72h; pick the morphology Different colonies were streaked to inoculate the pure culture, the colony morphology was observed and recorded, and a characteristic single colony with good growth was picked and placed in the MRS broth medium. Observe whether the broth becomes turbid, and store the turbid bacterial suspension in a refrigerator at 4°C, and then carry out the following ultraviolet mutagenesis screening experiment.
[0089] 2. Ultraviolet mu...
Embodiment 3
[0119] Embodiment 3, the preparation of fermented Chinese medicine preparation
[0120] 1. The preparation method of fermented traditional Chinese medicine in the early stage of growth
[0121] 15 parts of Codonopsis, 15 parts of Astragalus, 10 parts of Rhodiola, 10 parts of Cangzhu, 10 parts of Bergamot, 10 parts of Paeoniae Alba, 15 parts of honeysuckle, 15 parts of purslane, 20 parts of coix seed, 5 parts of amomum, according to weight Mix, then pulverize, cross a 300 mesh sieve, and mix for 15 minutes in a V-type mixer.
[0122] Add 90mL of saccharomyces (the number of viable bacteria is not less than 3 × 10 8 cfu / mL), 90mL Bacillus subtilis (the number of live bacteria is not less than 3×10 8 cfu / mL) of the bacterial culture solution, while adding 100 mL of 9% glucose solution, fully stirred and mixed evenly.
[0123] Put the mixture of traditional Chinese medicine compound and probiotics obtained in the above steps into a fermentation bag with a one-way valve, and fer...
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