Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Baker yeast B-n+M and preparation method and primer group thereof and application of preparation method and primer group to fermented unsweetened frozen dough

A technology of baker's yeast and primer pairs, which is applied in the field of fermentation, can solve problems such as difficult control of additive dosage, change of fermentation flavor, and death of bacteria, so as to improve trehalose synthesis ability and maltose metabolism ability, enhance freezing resistance, and improve seaweed The effect of sugar content

Inactive Publication Date: 2019-11-08
TIANJIN AGRICULTURE COLLEGE
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the quality of the product after long-term freezing of the dough is often severely reduced. The reason is that the baker’s yeast will withstand the stress of dehydration, ice crystals, freeze-thaw and other extreme environments during the low-temperature storage of the frozen dough, resulting in a large number of bacteria. The gas production capacity of the yeast is obviously reduced, the dough expansion is insufficient, and the quality deteriorates
In the modern production process, the fermentation time of frozen dough is usually shortened by increasing the amount of yeast input and using exogenous additives to ensure the expansion effect of frozen dough, but this often leads to changes in the fermentation flavor or difficult control of additive dosage. Potential food safety hazards and unstable product quality

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Baker yeast B-n+M and preparation method and primer group thereof and application of preparation method and primer group to fermented unsweetened frozen dough
  • Baker yeast B-n+M and preparation method and primer group thereof and application of preparation method and primer group to fermented unsweetened frozen dough
  • Baker yeast B-n+M and preparation method and primer group thereof and application of preparation method and primer group to fermented unsweetened frozen dough

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0053] The preparation method provided by the present invention includes transforming the gene knockout component into the starting yeast to knock out the neutral trehalase encoding gene NTH1; the gene knockout component contains a homologous arm for homologous recombination with the NTH1 gene, homologous The occurrence of recombination depends on the existence of a certain DNA sequence homology fragment between the gene knockout module and the target fragment, which is the homology arm on the gene knockout module, so the gene knockout module contains homology to the NTH1 gene The homology arm of the gene knockout component was transformed into the starting yeast, and the NTH1 gene in the starting strain was knocked out by homologous recombination. The NTH1 gene knocked out by the preparation method of the baker's yeast B-N+M provided by the present invention is 100% deleted, and it is difficult to generate back mutation.

[0054] The gene knockout assembly also includes an el...

Embodiment 1

[0072] The construction of embodiment 1 fermentation baker's yeast engineering bacterium

[0073] This example provides a strain of baker's yeast, named baker's yeast B-N+M-1, which lacks the neutral trehalase encoding gene NTH1 and overexpresses the maltase encoding gene MAL62 . Diploid BY-14 was haploidized to generate haploid strains 70a and 17α, and genetically engineered haploid strains were constructed by two homologous recombination methods, the KanMX resistance gene was removed and the haploid strains were hybridized to generate The diploid genetically engineered strain B-N+M-1, the construction process is as follows figure 1 shown.

[0074] The construction process of the recombinant plasmid pPGKM is as follows: figure 2 shown. The MAL62 gene was ligated with the pPGK1 plasmid containing a strong promoter to construct the pPGKM plasmid. get PGK1 P -MAL62-PGK1 T (PGKM) fragment, NKABM recombination process such as figure 2 shown. By PCR amplification technol...

Embodiment 2

[0087] Embodiment 2 Fusion PCR method constructs yeast strain

[0088] This example provides a strain of baker's yeast, using baker's yeast BY-14 as the starting baker's yeast strain, the baker's yeast lacks the neutral trehalase encoding gene NTH1, and overexpresses the maltase encoding gene MAL62, and the baker's yeast is named Bread The difference between yeast B-N+M-2 and Example 1 is that the deletion of the NTH1 gene and the overexpression of the MAL62 gene are achieved by the following methods:

[0089] First use primers as described in Table 1 to amplify respectively to obtain NA fragment, PGK1 P fragment, MAL62 fragment, PGK1 T Fragment, KanMX fragment and NB fragment, amplification system and amplification program are shown in Table 5:

[0090] table 5

[0091]

[0092] *Indicates that the annealing temperature is determined according to the optimal Tm value of the primer used; the annealing time is determined according to the size of the amplified product.

...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a baker yeast B-n+M and a preparation method and a primer group thereof and application of the preparation method and the primer group to a fermented unsweetened frozen dough, and relates to the technical field of yeasts. According to the baker yeast B-n+M, a neutral trehalase encoding gene NTH1 is deleted, and a maltase encoding gene MAL62 is overexpressed; a trehalose decomposition pathway can be partially cut off, meanwhile, the abilities of trehalose synthesis and maltose metabolism are improved, the content of trehalose in yeast cells is increased, then the freezingresistant properties of the yeast cells are enhanced, the fermentation performance before and after freezing can further be improved, and the technical problems that in the prior art, the baker yeasts are subjected to environmental stress during low-temperature storage of the frozen dough, the death of the bacteria is caused, after unfreezing, the gas production ability of the yeasts is declined,dough expansion is insufficient, and the quality becomes poor are solved.

Description

technical field [0001] The invention relates to the technical field of fermentation, in particular to a baker's yeast B-N+M, a preparation method thereof, a primer set and their application in fermenting sugar-free frozen dough. Background technique [0002] Baker's yeast (Saccharomyces cerevisiae), also known as Saccharomyces cerevisiae or budding yeast, belongs to the genus Saccharomyces and the species of Saccharomyces cerevisiae. The cell shape of Saccharomyces cerevisiae is usually oval or spherical. It is a eukaryotic single-celled microorganism. Yeast is rich in nutrients, such as protein, vitamins, amino acids, etc. Baker's yeast is used in the fermentation process of bread, which can use the nutrients in the flour to ferment and produce CO 2 So that the dough is fluffy and soft, and some flavor substances such as alcohol esters are produced, so that the bread is full of color, fragrance and taste. It is an important microbial leavening agent and loosening agent. ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/19C12N15/90C12N15/11A21D8/04C12R1/865
CPCA21D8/047C12N9/2402C12N15/905
Inventor 孙溪李瑞鹏刘海晴范志华张军肖萍刘珊娜王娜
Owner TIANJIN AGRICULTURE COLLEGE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com