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Bacillus subtilis strain NH-6 and application thereof

A technology of Bacillus subtilis and NH-6, which is applied in the direction of application, bacteria, and fungicides, can solve the problems of unsatisfactory effect and single plant disease control effect, and achieve strong stress resistance, simple cultivation method, and growth fast effect

Active Publication Date: 2019-11-08
NANTONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, many of the existing Bacillus subtilis can only play a preventive effect on a certain plant disease, and although some can prevent and treat multiple plant diseases, the effect is not ideal.

Method used

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  • Bacillus subtilis strain NH-6 and application thereof
  • Bacillus subtilis strain NH-6 and application thereof
  • Bacillus subtilis strain NH-6 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Isolation and Purification of Bacillus subtilis Strain NH-6

[0027] The Bacillus subtilis strain NH-6 in this example is obtained from the soil using the dilution plate method and the plate streaking method. Dissolved in water, the supernatant was diluted sequentially and coated on a flat plate, a single colony was picked for culture on a slant, and the pathogenic bacteria of sheath blight was used as an indicator bacterium for initial screening, secondary screening and quantitative screening.

[0028] Gram staining of the isolated Bacillus subtilis strain NH-6 was positive, and the thallus was rod-shaped. After being cultured in PYS medium for 24 hours at 26° C., spores were produced, and the spores were oval and mesogenic. At the initial stage of culture, the colonies are circular, milky white, with neat edges, and the colonies are raised in the shape of steamed buns, with a moist surface; at the later stage of culture, the colonies are light yellow, with d...

Embodiment 2

[0034] Example 2NH-6 bacterial strain 16S rDNA identification

[0035] The DNA of the NH-6 bacterial strain extracted in Example 1 is subjected to PCR amplification, and the amplified gene fragments are cloned and analyzed. The sequencing results show that the length of the 16S rDNA sequence of the bacterial strain is 1510bp. The sequencing results (GenBank accession number is DF520956) and The homology comparison of Bacillus 16S rDNA gene sequence in NCBI showed that the 16S rDNA sequence of the strain was 99% homologous to the 16S rDNA sequence of Bacillus subtilis. Based on the results of morphological characteristics and physiological and biochemical indicators of NH-6 strain, it was preliminarily determined that NH-6 was Bacillus subtilis.

Embodiment 3

[0036] Example 3 NH-6 bacterial strain has antagonistic effect on various plant diseases

[0037] The NH-6 strain was picked and transplanted to a PBGA slant, and cultured at 26°C for 36h. Bacteriostasis tests were carried out with sheath blight, black spot fungus and Botrytis cinerea as indicator bacteria, and the antagonism must be carried out under the same environmental conditions. Transplant the tested pathogenic bacteria on the activated PDA plate, culture at 26°C for 48 hours, then use a puncher to punch holes on the PDA plate with the bacteria, and inoculate the NH-6 strain on the non-bacterial PDA plate at a diagonal point, and grow on the plate after 10 hours. Transfer the bacteria block of the test bacteria into the center, culture at 26°C for 48 hours, put the plate of the pathogen block but not connected with NH-6 as a control, when the control is full of the plate, measure the width of the antagonistic band, and repeat the treatment 3 times for each pathogenic st...

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Abstract

The invention relates to the field of bacillus, in particular to a bacillus subtilis strain NH-6 and application thereof. The bacillus subtilis strain NH-6 is deposited at China General Microbiological Culture Collection Center with the preservation number of CGMCC 17815. The bacillus subtilis strain NH-6 has the advantages such as simple culture method, high growth rate, wide antibacterial spectrum and high resistance to stress. A biological control preparation prepared by the bacillus subtilis strain NH-6 can be used as a biological pesticide or a biological fertilizer to prevent and controlroot-knot nematode diseases and various plant pathogenic fungi including alternaria brassicae, banded sclerotial blight, botrytis cinerea and the like.

Description

technical field [0001] The invention relates to the field of bacillus, in particular to a bacillus subtilis strain NH-6 and an application thereof. Background technique [0002] Bacillus subtilis is a kind of Bacillus genus, which is widely distributed in soil and decayed organic matter, and is easy to reproduce in subtilis juice. Bacillus subtilis is widely used in feed, fertilizer and as a fungicide. Its preparation is a non-toxic, non-residue and non-polluting green bacterial agent, which has broad development prospects and shows huge social and ecological benefits. [0003] However, many of the existing Bacillus subtilis can only prevent and treat a certain plant disease, and some of them can prevent and treat multiple plant diseases, but the effect is not ideal. Therefore, continue to invent a new Bacillus subtilis that can efficiently prevent and treat various plant diseases. Contents of the invention [0004] The object of the present invention is to provide a kin...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P5/00A01P3/00C05F11/08C05G3/00C05G3/02C12R1/125
CPCC12N1/20A01N63/00C05F11/08C05G3/00C12R2001/125C12N1/205
Inventor 李德全邓自发曹云英谈蓉
Owner NANTONG UNIVERSITY
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