Photoelectrochemical biosensor and detection method thereof for BLM (bleomycin)

A biosensor and photoelectrochemical technology, applied in the direction of chemiluminescence/bioluminescence, material electrochemical variables, scientific instruments, etc., can solve the problems of reducing active sites and adsorption capacity, and achieve improved catalytic activity, good stability, and electrolyte diffusion good sex effect

Active Publication Date: 2019-11-08
QUFU NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the low density of atoms in the structure of MOFs reduces their active sites and adsorption capacity

Method used

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  • Photoelectrochemical biosensor and detection method thereof for BLM (bleomycin)
  • Photoelectrochemical biosensor and detection method thereof for BLM (bleomycin)
  • Photoelectrochemical biosensor and detection method thereof for BLM (bleomycin)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] (1) Add 0.8g Na 2 WO 3 2H 2 O was dissolved in 18 mL of deionized water, and HCl was added dropwise to adjust the pH to 1.1. 0.8g H 2 C 2 o 4 Add to the above mixture, dilute to 45 mL and stir at room temperature. Subsequently, a total of 0.8 g NH 4 Cl and 0.8 g of thiourea were dissolved in the above mixture, respectively. Clean the TM by sonicating in water and ethanol for 8 min. The pretreated titanium mesh (TM) (2 × 4 cm) and the above solution were transferred into a 50 mL Teflon-lined stainless steel autoclave and kept at 170 °C for 15 h. After cooling to room temperature, the product was washed three times with ultrapure water. Subsequently, the sample was placed under flowing N 2 It was heated at 440° C. for 0.8 hours in an atmosphere. Then get WS 2 / TM.

[0043] (2) Add 0.8mL HAuCl 4 (22mM) solution was added to 98mL distilled water and boiled. Then, 1.9mLNa 3 C 6 h 5 o 7 2H 2 O solution (67mM) was quickly added to HAuCl 4 In the boiling so...

Embodiment 2

[0047] (1) Add 0.9g Na 2 WO 3 2H 2 O was dissolved in 22 mL of deionized water, and HCl was added dropwise to adjust the pH to 1.3. 0.9g H 2 C 2 o 4 Add to the above mixture, dilute to 45-50 mL and stir at room temperature. Subsequently, a total of 0.82 g NH 4 Cl and 0.84 g of thiourea were separately dissolved in the above mixture. TMs were cleaned by sonication in water and ethanol for 12 min. The pretreated titanium mesh (TM) (2 × 4 cm) and the above solution were transferred into a 50 mL Teflon-lined stainless steel autoclave and kept at 190 °C for 17 h. After cooling to room temperature, the product was washed three times with ultrapure water. Subsequently, the sample was placed under flowing N 2 It heated at 460 degreeC for 1.2 hours in atmosphere. Then get WS 2 / TM.

[0048] (2) Add 1.2mL HAuCl 4 (26mM) solution was added to 100mL distilled water and boiled. Then, 2.1mLNa 3 C 6 h 5 o 7 2H 2 O solution (69 mM) was quickly added to HAuCl 4 In the boil...

Embodiment 3

[0052] (1) Add 0.85g Na 2 WO 3 2H 2 O was dissolved in 20 mL of deionized water, and HCl was added dropwise to adjust the pH to 1.2. 0.85g H 2 C 2 o 4 Add to the above mixture, dilute to 48 mL and stir at room temperature. Subsequently, a total of 0.81 g NH 4 Cl and 0.83 g of thiourea were separately dissolved in the above mixture. Clean the TM by sonicating in water and ethanol for 10 min. The pretreated titanium mesh (TM) (2 × 4 cm) and the above solution were transferred into a 50 mL Teflon-lined stainless steel autoclave and kept at 180 °C for 16 h. After cooling to room temperature, the product was washed three times with ultrapure water. Subsequently, the sample was placed under flowing N 2 It heated at 450 degreeC for 1.0 hour in atmosphere. Then get WS 2 / TM.

[0053] (2) Add 1.0mL HAuCl 4 (22-26mM) solution was added to 99mL of distilled water and brought to a boil. Then, 2.0mLNa 3 C 6 h 5 o 7 2H 2 O solution (68 mM) was quickly added to HAuCl 4 I...

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Abstract

The invention belongs to the technical field of nanometer new materials, and particularly relates to an application and preparation that a WS<2> nanorod array and a gold nanoparticle (WS<2>-Au) are taken as photoelectrochemical matrixes and an (Ag) / ZnMOF analog peroxide nanocomposite is taken as a signal amplifier. A photoelectrochemical biosensor comprises a working electrode, a reference electrode and a counter electrode, wherein the working electrode, the reference electrode and the counter electrode are connected with a photoelectrochemical workstation. The photoelectrochemical biosensor is characterized in that the working electrode is modified with the WS<2>-Au, and in addition, (Ag) / ZnMOF is connected to the surface of an electrode through hybridization chain reaction. On the basisof the utilization of the WS<2>-Au and the (Ag) / ZnMOF analog peroxide nanocomposite, a simple PEC (photoelectrochemical) bioanalysis platform used for monitoring BLM activity is designed. An experiment proves that the constructed photoelectrochemical biosensor platform is simple and economic, and exhibits high sensitivity, selectivity and reliability for BLM detection. The work is a basis of a newuniversal PEC immunoassay format, and can be expanded to be used for detecting other interested biological interactions.

Description

technical field [0001] The invention belongs to the technical field of new nanometer materials, and in particular relates to a photoelectrochemical biosensor and a detection method for BLM. Background technique [0002] As a natural glycopeptide-derived antibiotic, bleomycin (BLM) has been widely used as a chemotherapeutic drug for the treatment of various cancers. To date, various techniques for BLM detection have been established, such as electrochemical methods, fluorescence, and electrochemiluminescence. However, these above-mentioned methods usually suffer from complicated sample handling or expensive or toxic reagents. There is an urgent need to develop a sensitive and efficient method for quantitative determination of BLM in chemotherapeutic drugs and clinical samples. [0003] As an emerging analytical method, photoelectrochemical (PEC) sensing has attracted much attention in recent years. It realizes the absolute separation of input signal and output signal, and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/416G01N27/327G01N27/30G01N21/76C08G83/00B82Y30/00B22F9/16
CPCG01N27/416G01N27/301G01N27/327G01N21/76B22F9/16B82Y30/00C08G83/008B22F2009/165
Inventor 渠凤丽孔维素李钦谭青青
Owner QUFU NORMAL UNIV
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