Application of polysaccharide substance in preparation of dilution solution for freeze preservation of goat sperms and preparation method of dilution solution,
A technology for cryopreservation and dilution, which is used in the preservation, application, and animal husbandry of human or animal bodies to improve reproductive performance, economic benefits, and sperm motility.
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Embodiment 1
[0029] This embodiment provides a diluent for goat sperm cryopreservation, including fungus polysaccharide, fructose, trisodium citrate, citric acid, sodium bicarbonate, lecithin, dimethyl sulfoxide, glycerin, penicillin and streptomycin. Its specific preparation method is as follows:
[0030] (1) Preparation of liquid A: Accurately weigh 0.25g of trisodium citrate, 0.75g of citric acid, and 0.062g of sodium bicarbonate with an analytical balance, add 50mL of sterile ultrapure water to dissolve, and use 1mol / L sodium hydroxide or 1mol / L Adjust the pH to 7 with L hydrochloric acid, and adjust the volume to 100 mL to obtain the so-called A liquid.
[0031] (2) Preparation of liquid B: accurately weigh 0.5g of fructose and 0.125g of lecithin with an analytical balance, accurately measure 6mL of dimethyl sulfoxide and 6mL of glycerin, add 50mL of liquid A to dissolve, and use 1mol / L sodium hydroxide or 1mol / L hydrochloric acid to adjust the pH to 7, and dilute to 100mL to obtain...
Embodiment 2
[0048] This embodiment provides a diluent for goat sperm cryopreservation, including fungus polysaccharide, fructose, trisodium citrate, citric acid, sodium bicarbonate, lecithin, dimethyl sulfoxide, glycerin, penicillin and streptomycin. The specific operation is the same as in Example 1, except that the dosage of each component is 1.8g of fungus polysaccharide, 4g of fructose, 2g of trisodium citrate, 6g of citric acid, 0.5g of sodium bicarbonate, 1g of lecithin, and 6mL of dimethyl sulfoxide. , glycerin 6mL, penicillin 0.07g and streptomycin 0.06g.
Embodiment 3
[0052] This embodiment provides a diluent for goat sperm cryopreservation, including fungus polysaccharide, fructose, trisodium citrate, citric acid, sodium bicarbonate, lecithin, dimethyl sulfoxide, glycerin, penicillin and streptomycin. The specific operation is the same as in Example 1, except that the dosage of each component is 1.2g of fungus polysaccharide, 2.25g of fructose, 1.25g of trisodium citrate, 3.35g of citric acid, 0.28g of sodium bicarbonate, 0.53g of lecithin, and dimethyl 18 mL of sulfoxide, 18 mL of glycerol, 0.25 g of penicillin and 0.25 g of streptomycin.
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