Application of polysaccharide substance in preparation of dilution solution for freeze preservation of goat sperms and preparation method of dilution solution,

A technology for cryopreservation and dilution, which is used in the preservation, application, and animal husbandry of human or animal bodies to improve reproductive performance, economic benefits, and sperm motility.

Inactive Publication Date: 2019-11-15
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the cryopreservation technology of goat semen has bee

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] This embodiment provides a diluent for goat sperm cryopreservation, including fungus polysaccharide, fructose, trisodium citrate, citric acid, sodium bicarbonate, lecithin, dimethyl sulfoxide, glycerin, penicillin and streptomycin. Its specific preparation method is as follows:

[0030] (1) Preparation of liquid A: Accurately weigh 0.25g of trisodium citrate, 0.75g of citric acid, and 0.062g of sodium bicarbonate with an analytical balance, add 50mL of sterile ultrapure water to dissolve, and use 1mol / L sodium hydroxide or 1mol / L Adjust the pH to 7 with L hydrochloric acid, and adjust the volume to 100 mL to obtain the so-called A liquid.

[0031] (2) Preparation of liquid B: accurately weigh 0.5g of fructose and 0.125g of lecithin with an analytical balance, accurately measure 6mL of dimethyl sulfoxide and 6mL of glycerin, add 50mL of liquid A to dissolve, and use 1mol / L sodium hydroxide or 1mol / L hydrochloric acid to adjust the pH to 7, and dilute to 100mL to obtain...

Embodiment 2

[0048] This embodiment provides a diluent for goat sperm cryopreservation, including fungus polysaccharide, fructose, trisodium citrate, citric acid, sodium bicarbonate, lecithin, dimethyl sulfoxide, glycerin, penicillin and streptomycin. The specific operation is the same as in Example 1, except that the dosage of each component is 1.8g of fungus polysaccharide, 4g of fructose, 2g of trisodium citrate, 6g of citric acid, 0.5g of sodium bicarbonate, 1g of lecithin, and 6mL of dimethyl sulfoxide. , glycerin 6mL, penicillin 0.07g and streptomycin 0.06g.

Embodiment 3

[0052] This embodiment provides a diluent for goat sperm cryopreservation, including fungus polysaccharide, fructose, trisodium citrate, citric acid, sodium bicarbonate, lecithin, dimethyl sulfoxide, glycerin, penicillin and streptomycin. The specific operation is the same as in Example 1, except that the dosage of each component is 1.2g of fungus polysaccharide, 2.25g of fructose, 1.25g of trisodium citrate, 3.35g of citric acid, 0.28g of sodium bicarbonate, 0.53g of lecithin, and dimethyl 18 mL of sulfoxide, 18 mL of glycerol, 0.25 g of penicillin and 0.25 g of streptomycin.

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Abstract

The invention discloses an application of a polysaccharide substance in preparation of a dilution solution for freeze preservation of goat sperms and a preparation method of the dilution solution. Thepolysaccharide substance comprises one or more selected from the group consisting of tremella polysaccharide, lentinan, auricularia polysaccharide and cordyceps polysaccharide. The invention also provides the dilution solution for freeze preservation of the goat sperms. The dilution solution comprises the polysaccharide substance, a nutrient substance, a buffer substance and the like. The dilution solution for freeze preservation of the goat sperms provided by the invention has the effects of improving a sperm motility rate, an acrosome and plasma membrane integrity rate after freeze preservation, and significantly improving goat reproductive performance and economic benefits.

Description

technical field [0001] The invention belongs to the field of animal biotechnology and relates to animal reproduction technology, in particular to the application and preparation method of polysaccharides used to prepare diluents for goat semen preservation. Background technique [0002] Animal semen cryopreservation is the special treatment of semen, stored at ultra-low temperature, in order to achieve the purpose of long-term preservation. However, in the process of cooling and cryopreservation, sperm are prone to various damages, including physical, chemical and biological damage. Compared with fresh semen, the sperm survival rate, sperm viability rate, acrosome integrity rate, mitochondrial activity and conception rate of the frozen-thawed semen were significantly reduced, especially for goat semen, which is of great importance to production practice and It is an extremely unfavorable factor for scientific research. Although the cryopreservation technology of goat semen...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0221A01N1/0226
Inventor 王建刚李广安小鹏张磊张效川郭丹补琪琪杨少华刘囧延刘进亿
Owner NORTHWEST A & F UNIV
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