KASP-based rat genetic quality monitoring SNP marker typing method and kit

A typing method and quality monitoring technology, applied in biochemical equipment and methods, DNA/RNA fragments, recombinant DNA technology, etc., can solve the problems of cumbersome methods, high prices, and difficulty in automatic application, and achieve accurate conclusions in a short time and low economic cost
CN110452969AActive Publication Date: 2019-11-15SUZHOU XISHAN BIOLOGICAL TECH
5 Cites 5 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SUZHOU XISHAN BIOLOGICAL TECH
Publication Date
2019-11-15

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention belongs to the technical field of biology and particularly relates to a KASP-based rat genetic quality monitoring SNP marker typing method and a kit. There are 48 SNP loci which can be detected, wherein the loci cover 20 autosomes and X sex chromosomes of a rat, at least two SNP loci are selected on each chromosome, and at least 5 SNP loci are used for distinguishing every two inbredrat strains. The method also comprises a KASP primer set and a KASP premix which are designed for a rat SNP marker. According to the method, common inbred rat strains can be effectively and rapidly distinguished, genetic homozygosity or genetic contamination of inbred rats can be effectively detected, genetic diversity of rats in a closed colony can be rapidly detected, and the rats unknown in source can be traced. The method has the advantages of high intuition, accuracy and flexibility and low cost and is suitable for automation and batch detection.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention belongs to the field of biotechnology, and in particular relates to a KASP-based SNP marker typing method and kit for monitoring the genetic quality of rats. Background technique

[0002] In biomedical research, the genetic quality of experimental animals is an important factor to measure its quality. The use of experimental animals with stable genetic characteristics can ensure that the experimental results have reference value and comparability. "Genome and SNP Genetic Marker Analysis of Commonly Used Strains of Rats" (South China University of Technology, Master of Engineering Thesis, April 2018) mentioned that regular genetic testing is essential: inbred animals isogenic The characteristics of sex, long-term genetic stability, uniformity, individuality, background information and relatively complete data can detect the homozygosity and genetic contamination of inbred animals, providing a basis for ensuring the reliability, stability an...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More