Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Foot-and-mouth disease virus-like particle antigen, vaccine composition prepared therefrom, and preparation method and application thereof

A technology of foot-and-mouth disease virus and vaccine composition, which is applied in the field of veterinary biological products, can solve the problems of inability to protect against various topological O-type foot-and-mouth disease viruses, inability to adapt to the healthy development of animal husbandry, and inability to completely eliminate FMDV. Biosafety risk, good immune efficacy, low production cost effect

Active Publication Date: 2022-08-09
PU LIKE BIO ENG
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no cross-protection between the topological types of O-type FMD. The current single O-type FMD vaccine cannot provide effective immune protection against multiple topological O-type FMD viruses. So that FMDV can not be completely eradicated
The vaccines currently used for prevention and control can no longer adapt to the healthy development of the current animal husbandry industry, and there is an urgent need for new vaccines that can simultaneously prevent and control multiple topological types of FMD infection

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Foot-and-mouth disease virus-like particle antigen, vaccine composition prepared therefrom, and preparation method and application thereof
  • Foot-and-mouth disease virus-like particle antigen, vaccine composition prepared therefrom, and preparation method and application thereof
  • Foot-and-mouth disease virus-like particle antigen, vaccine composition prepared therefrom, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach

[0023] As an embodiment of the present invention, the pharmaceutically acceptable carrier includes an adjuvant, and the adjuvant includes: (1) aluminum glue adjuvant, saponin, avridine, DDA; (2) water-in-oil emulsion , oil-in-water emulsions, water-in-oil-in-water emulsions; or (3) polymers of acrylic or methacrylic acid, copolymers of maleic anhydride and alkenyl derivatives; and RIBI adjuvant systems, Block co- One or more of polymer, SAF-M, monophosphoryl lipid A, Avridine lipid-amine adjuvant, E. coli heat labile enterotoxin, cholera toxin, IMS 1314, muramyl dipeptide, and Gel adjuvant kind;

[0024] Preferably, the saponin is Quil A, QS-21, GPI-0100;

[0025] Preferably, the emulsion is an SPT emulsion, a MF59 emulsion, or the emulsion is formed by combining an oil with an emulsifier, and the emulsion may be based on light liquid paraffin oil, isoprenoid oil (such as squalane or squalene) produced by oligomerization of olefins Oils, olefins, especially oils resulting fr...

Embodiment 1

[0041]Example 1 Expression of foot-and-mouth disease virus-like particles

[0042] 1. Construction of Expression Vectors

[0043] The VP4 gene fragment shown in SEQ ID NO.1 in the sequence listing, the VP2 gene fragment shown in the sequence listing SEQ ID NO.2, the VP3 gene fragment shown in the sequence listing SEQ ID NO.3, and the SEQ ID NO.3 in the sequence listing were synthesized by Jinweizhi Company. The VP1 gene fragments shown in ID NO.4 were respectively connected with the pBLUE-T Vector vector, and the successfully connected recombinant clones were respectively treated with BamH I / EcoR I, PstI / XbaI, Sac I / Sal I, Hind III / Xho I enzymes The digested fragment was ligated with the pET28a vector digested by the same enzyme to obtain a positive clone pET28a-VP4-VP2-VP3-VP1 inserted into the VP4, VP2, VP3 and VP1 genes of the O-type foot-and-mouth disease virus. Transform the ligated plasmid into CaCl 2 The prepared DH5α competent cells were spread on kanamycin-resistant...

Embodiment 2

[0051] Example 2 Preparation of foot-and-mouth disease virus-like particle vaccine composition

[0052] The virus-like particles prepared in Example 1 were slowly added to the adjuvant. During the addition, the mixture was continuously stirred for 12 minutes with an emulsifier with a rotating speed of 800 rpm, mixed well, and stored at 4°C, which was the foot-and-mouth disease virus-like particle vaccine composition. The specific ratio is shown in Table 1. Adjuvants suitable for use in the present invention may be adjuvants known to those skilled in the art. In the present example, the adjuvant ISA206 (France Saibiq) was selected.

[0053] Table 1 Composition ratio of foot-and-mouth disease virus-like particle vaccine composition

[0054] Vaccine 1 Vaccine 2 Vaccine 3 Foot and mouth disease antigen (μg / ml) 160 200 240 ISA 206 adjuvant (V / V%) 50％ 50％ 50％

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention provides a foot-and-mouth disease virus-like particle antigen, which is assembled from O-type foot-and-mouth disease virus VP4, VP2, VP3 and VP1 antigenic proteins. The present invention also provides a foot-and-mouth disease virus-like particle vaccine composition comprising an immune amount of the foot-and-mouth disease virus-like particle antigen, and a preparation method and application thereof. The immunogenicity and immunogenicity comparison test proves that the foot-and-mouth disease virus-like particle vaccine composition has good protection ability, and can prevent and control multiple topological type O foot-and-mouth disease infections at the same time.

Description

technical field [0001] The present invention relates to the field of veterinary biological products. More specifically, the present invention relates to a foot-and-mouth disease virus-like particle antigen, a vaccine composition prepared therefrom, and a preparation method and application thereof. Background technique [0002] Foot-and-mouth disease (FMD) is an acute, highly contagious animal disease that can spread rapidly over long distances. significant economic losses worldwide. Animals affected by foot-and-mouth disease include cattle, sheep and pigs. The causative agent, foot-and-mouth disease virus (FMDV), is an aphthous virus of the Picornavirus family. The virus is divided into 7 serotypes (A, O, C, Asia 1, SAT1, SAT2 and SAT3), of which type O foot-and-mouth disease virus is the most prevalent. According to genetic classification, there are currently three Topotypes of FMD virus in China, which belong to CATHAY type (China type), ME-SA type (Middle East-South A...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/09C12N15/42C12N15/70A61K39/135A61P31/14
CPCC07K14/005A61K39/12A61P31/14C12N2770/32123C12N2770/32134Y02A50/30Y02A40/70
Inventor 田克恭肖燕逄文强孙进忠张许科
Owner PU LIKE BIO ENG
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com