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Method for co-culturing inonotus obliquus, ganoderma lucidum, and phellinus linteus mycelia

A technology of Phellinus mycelium and cultivation method, which is applied in the direction of botany equipment and methods, cultivation, fungi, etc., can solve problems such as danger, abnormal growth, pituitary gland damage, etc., and achieve excellent taste and flavor, and excellent health functional effect

Inactive Publication Date: 2019-11-19
GIUNCHAN CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, nitrite, the main added ingredient of the salt pickling agent, is more dangerous when taken in large quantities as a synthetic food additive
Moreover, sodium glutamate used as a salting agent additive is an excitatory neurotransmitter. When glutamic acid is absorbed by nerve tissue in large quantities, it will destroy nerve cell membranes. Causes damage to the pituitary gland, causing it not only to fail to grow normally, but also to cause abnormalities in its general metabolism

Method used

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  • Method for co-culturing inonotus obliquus, ganoderma lucidum, and phellinus linteus mycelia
  • Method for co-culturing inonotus obliquus, ganoderma lucidum, and phellinus linteus mycelia
  • Method for co-culturing inonotus obliquus, ganoderma lucidum, and phellinus linteus mycelia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048]

[0049] After the fruiting body tissues of Chaga, Ganoderma and Phellinus were isolated and inoculated on potato dextrose agar, the hyphae of Chaga, Ganoderma and Phellinus were cultured at 27-29°C for 2 weeks. ready to After subdividing the potato dextrose broth medium for the unit, use a knife to cut each Chaga, Ganoderma lucidum, and Phellinus hyphae cultivated on the potato dextrose agar into 1 square mm, and place them in each medium containing the potato dextrose broth. In a Erlenmeyer flask, 5 slices were inoculated with the cut three strains.

[0050] In a BOD incubator (Bio-Oxygen Demand incubator, low-temperature incubator) under the conditions of 27-28°C and humidity 20%, the culture medium of the compound inoculation was cultured statically for 1 week, and stirred for about 1 minute every day during the culture period. After 1 week, the flask in the culture after compound inoculation was moved to a shaking incubator and cultured at 27° C. and 100 rpm ...

Embodiment 2

[0054]

[0055] In every 100g of pork tenderloin, mix the finely cut compound culture mycelia, cumin powder, basil powder, dill herb powder, dill seed powder and salt from Example 1 and mix evenly, and store at room temperature at 25°C After 4 hours, it was placed again in a low-temperature warehouse at 7°C for 24 hours to make salt-cured meat.

[0056] [Table 1]

[0057]

experiment example 1

[0078]

[0079] Commissioned the Korea Institute of Analytical Technology Co., Ltd. to check the content of β-glucan in Chaga, Ganoderma lucidum and Phellinus hyphae. The results are shown in Table 5, the compound culture hyphae cultured according to the method of Example 1 had the highest content of β-glucan.

[0080] [table 5]

[0081] condition β-glucan content (mg / m) Example 1 196.1 Comparative example 1-1 100.6 Comparative example 1-2 102.3 Comparative example 1-3 102.5 Comparative example 1-4 112.4 Comparative example 1-5 124.4 Comparative example 2 149.2 Comparative example 3 131.4 Comparative example 4 136.2

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Abstract

The present invention relates to a method for co-culturing Inonotus obliquus, Ganoderma lucidum, and Phellinus linteus. The co-cultured mycelia, prepared by the method, has excellent health functionality thanks to the high beta glucan content thereof and can be used as an additive or a cooking condiment for various foods. In addition, the use of the co-cultured mycelia in curing raw meat allows the easy preparation of meat foods that have a good taste and flavor.

Description

technical field [0001] The invention discloses a composite culture method of chaga, ganoderma lucidum and Phellinus mycelium. Background technique [0002] Chaga (Inonotus obliquus) is a sclerotium parasitic on birch trees, alder trees and other trees that grow in cold regions such as Russia, Canada, and Hokkaido, Japan. Recently, Japan reported that chaga has the effect of preventing hepatitis C and treating liver cancer. The United States also classifies chaga as a special natural substance and strives to develop it as a future pharmaceutical and health food. South Korea is also using chaga for gastric cancer patients and diabetic patients and found that its effect is better than other mushrooms. In addition, there are reports that chaga also has the effects of improving body resistance, inhibiting tumor occurrence, and whitening. [0003] Phellinus linteus (Phellinus linteus) is also known as Phellinus linteus, and Donggui Bogam (a famous Korean ancient book on traditio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A23L31/00A23L27/10A23L13/74
CPCA01G18/50A01G18/10A23L13/424A23L13/46A23L31/10A23L27/88A22C9/00A23L33/10A23L33/145A23L13/74A23L27/10A23L31/00C12N1/14A23V2002/00A01G18/20
Inventor 朴种礼
Owner GIUNCHAN CO LTD
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