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A kind of self-crosslinking hydrogel with synergistic action of sugar and enzyme and preparation method thereof

A hydrogel, self-crosslinking technology, applied in biochemical equipment and methods, enzymes, bandages, etc., can solve the problem that chemical initiators or crosslinking agents are difficult to remove, difficult to ensure structural uniformity, and hydrogel products are difficult to meet Field requirements and other issues to achieve good biocompatibility, mild reaction conditions, and low prices

Active Publication Date: 2021-12-07
广东省科学院生物与医学工程研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the residual chemical initiators or cross-linking agents are difficult to remove, making it difficult for hydrogel products to meet the requirements of pharmaceuticals, food and other fields, and it is difficult to ensure the uniformity of the structure

Method used

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  • A kind of self-crosslinking hydrogel with synergistic action of sugar and enzyme and preparation method thereof
  • A kind of self-crosslinking hydrogel with synergistic action of sugar and enzyme and preparation method thereof
  • A kind of self-crosslinking hydrogel with synergistic action of sugar and enzyme and preparation method thereof

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Experimental program
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preparation example Construction

[0030] A preparation method of self-crosslinking hydrogel, comprising the following steps:

[0031] Mixing carboxymethyl chitosan, glucose, glucose oxidase and peroxidase in water, standing still to obtain self-crosslinking hydrogel.

[0032] Preferably, the following steps are included:

[0033] Dissolving carboxymethyl chitosan in water is A solution; glucose, glucose oxidase and peroxidase are enzymatically hydrolyzed in water to obtain B solution, then A solution and B solution are mixed, and the cross-linked hydrogel is obtained by standing still.

[0034] Preferably, the following steps are included:

[0035] (1) Glucose and carboxymethyl chitosan are mixed to obtain C solution;

[0036] (2) Glucose oxidase and peroxidase are dissolved in water to obtain D solution;

[0037] (3) Mix C and D solutions and let stand to obtain the cross-linked hydrogel.

[0038] Preferably, the peroxidase is at least one of catalase and horseradish peroxidase.

[0039] Preferably, the ...

Embodiment 1

[0047] A solution: 1mL1.8% carboxymethyl chitosan solution

[0048] Solution B: 0.1514g glucose, 0.5mL ultrapure water, 0.0025g glucose oxidase, 0.0025g catalase

[0049] Gel time: 4 to 5 minutes

[0050] Preparation method: Dissolve 1mL of 1.8% carboxymethyl chitosan in water to obtain solution A; 0.1514g of glucose, 0.0025g of glucose oxidase, and 0.0025g of hydrogen peroxide are enzymatically hydrolyzed in 0.5mL of water to obtain solution B; solution A and Solution B was mixed and allowed to stand for 4-5 minutes to obtain a cross-linked hydrogel.

Embodiment 2

[0052] Solution A: 1 mL of 1.4% carboxymethyl chitosan solution

[0053] Solution B: 0.1514g glucose, 0.5mL ultrapure water, 0.0025g glucose oxidase, 0.0025g catalase

[0054] Gel time: 4 to 5 minutes

[0055] Preparation method: Dissolve 1mL of 1.4% carboxymethyl chitosan in water to obtain solution A; 0.1514g of glucose, 0.0025g of glucose oxidase, and 0.0025g of hydrogen peroxide are enzymatically hydrolyzed in 0.5mL of water to obtain solution B; solution A and Solution B was mixed and allowed to stand for 4-5 minutes to obtain a cross-linked hydrogel.

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Abstract

The invention discloses a self-crosslinked hydrogel with synergistic effect of sugar and enzyme and a preparation method thereof. The preparation method comprises: mixing carboxymethyl chitosan, glucose, glucose oxidase and peroxidase in water, and standing to form Self-crosslinking hydrogels. The raw material of the present invention is natural and easy to obtain, good biocompatibility, and non-toxic; the preparation method of the hydrogel is simple, quick and gentle, easy to realize industrial production, low in preparation cost, stable in properties, easy to smear, and does not need to add toxic small molecules in the preparation process Cross-linking agent, initiator and catalyst, with mild reaction conditions and good biocompatibility. The hydrogel of the present invention can be used as a novel moist wound dressing, has good transparency, can visually observe the condition of the wound, and does not adhere to the wound tissue.

Description

technical field [0001] The invention belongs to the field of biomedical materials, in particular to a self-crosslinked hydrogel with synergistic effect of sugar and enzyme and a preparation method thereof. Background technique [0002] Hydrogel is a solid or semi-solid material with a three-dimensional polymer network. It is soft in texture and high in water content. It is similar to biological tissue and has good biocompatibility. It can be used in drug controlled release systems and wound dressings. and tissue engineering and other biomedical fields. [0003] Hydrogel synthesis methods mainly include physical cross-linking and chemical cross-linking. The preparation of chemical cross-linking needs to add some chemical initiators or cross-linking agents that are harmful to the human body and the environment, so that the hydrophilic polymers can be cross-linked by chemical bonds to form a three-dimensional network structure. However, the residual chemical initiators or cro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/00A61L26/00
CPCA61L26/0023A61L26/0047A61L26/0061A61L26/0066A61L26/008A61L2300/232A61L2300/236A61L2300/254A61L2300/404C08L5/08C08L89/00
Inventor 裴大婷刘群峰于珊周小雁耿志杰
Owner 广东省科学院生物与医学工程研究所