Method for efficiently and rapidly retrieving green and improving soil pH value by spray-seeding robinia pseudoacacia on exposed rock wall of shale
A technology for improving soil and rock walls, applied in the fields of land preparation methods, soil conditioning materials, chemical instruments and methods, etc., can solve problems such as the decline of soil drought resistance, reduce root system, soil water loss, etc., and achieve the improvement of organic matter content and pH. The effect of improving the value and reducing the application amount
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Embodiment 1
[0031] 1) Acquisition and identification of strains
[0032] Soil samples were collected from 10 cm of rhizosphere soil on both sides of Yueyang Avenue, Yueyang City, using the dilution coating plate method, in a 35 ° C incubator, with nutrient agar solid medium (NA medium: 10 g of peptone; 3 g of beef powder; Sodium chloride 5g; Agar 15g; Sterile water 1000mL; pH value 7) Cultivate for 2-3 days, pick different colonies by visual observation, and repeatedly streak and purify to obtain several single colonies of different species.
[0033] Select a single colony to make a plate, and send it to Shanghai Jinyu Medical Laboratory for sequencing to obtain the 16S rDNA gene sequence, as shown in SEQID NO.1. The measured 16S rDNA gene sequence was compared with the sequence in the GenBank database by BLAST. The results showed that the similarity between the strain and Kocuria polaris was 99.32%. Combined with morphological characteristics (yellow in the center, light yellow at the ...
Embodiment 2
[0041] 1. Preparation of microbial mixture
[0042] 1) Take Koukia X-22, Microbacterium X-26, Bacillus X-28 strains, and Microbacterium X-18, respectively, on nutrient agar solid medium, activate at 35°C for 24 hours;
[0043] 2) Pick a ring of sludge from the activated Microbacterium X-26, Bacillus X-28 and Microbacterium X-18 with an inoculation loop and add them to the LB liquid medium respectively. 22 into the NA liquid medium, 35 ° C, 200r / min constant temperature shaking for 24 hours to prepare the seed liquid; the pH value of the seed liquid changes are shown in Table 2;
[0044] Table 2 pH change results before and after seed solution
[0045] pH before culture pH after incubation culture medium Microbacterium sp. X-18 6.8-7.0 4.78 LB Koukia X-22 6.8-7.0 8.35 NA Microbacterium sp. X-26 6.8-7.0 8.77 LB Bacillus X-28 6.8-7.0 8.39 LB
[0046] 3) Take the seed liquid according to the inoculation amount of 3% and ino...
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