Method for efficiently and rapidly retrieving green and improving soil pH value by spray-seeding robinia pseudoacacia on exposed rock wall of shale

A technology for improving soil and rock walls, applied in the fields of land preparation methods, soil conditioning materials, chemical instruments and methods, etc., can solve problems such as the decline of soil drought resistance, reduce root system, soil water loss, etc., and achieve the improvement of organic matter content and pH. The effect of improving the value and reducing the application amount

Active Publication Date: 2019-12-03
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Soil compaction will cause the roots of crops to have difficulty breathing, thereby reducing the number of roots, the shape becomes short and thick, and the roots are not deep, which will prevent the plants from getting sufficient water and nutrients
In addition, the compacted soil will also accelerate the loss of soil moisture, and the soil's drought resistance will also decrease, which will not be conducive to the growth of plants.
[0005] In the prior art, lime is usually used to neutralize the active and latent acids in the soil, but studies have shown that no matter whether lime is applied or not, soil reacidification exists, but the application of lime accelerates the reacidification of soil
In addition, excessive lime application can also cause problems such as soil compaction or nutrient loss.

Method used

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  • Method for efficiently and rapidly retrieving green and improving soil pH value by spray-seeding robinia pseudoacacia on exposed rock wall of shale
  • Method for efficiently and rapidly retrieving green and improving soil pH value by spray-seeding robinia pseudoacacia on exposed rock wall of shale

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Experimental program
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Effect test

Embodiment 1

[0031] 1) Acquisition and identification of strains

[0032] Soil samples were collected from 10 cm of rhizosphere soil on both sides of Yueyang Avenue, Yueyang City, using the dilution coating plate method, in a 35 ° C incubator, with nutrient agar solid medium (NA medium: 10 g of peptone; 3 g of beef powder; Sodium chloride 5g; Agar 15g; Sterile water 1000mL; pH value 7) Cultivate for 2-3 days, pick different colonies by visual observation, and repeatedly streak and purify to obtain several single colonies of different species.

[0033] Select a single colony to make a plate, and send it to Shanghai Jinyu Medical Laboratory for sequencing to obtain the 16S rDNA gene sequence, as shown in SEQID NO.1. The measured 16S rDNA gene sequence was compared with the sequence in the GenBank database by BLAST. The results showed that the similarity between the strain and Kocuria polaris was 99.32%. Combined with morphological characteristics (yellow in the center, light yellow at the ...

Embodiment 2

[0041] 1. Preparation of microbial mixture

[0042] 1) Take Koukia X-22, Microbacterium X-26, Bacillus X-28 strains, and Microbacterium X-18, respectively, on nutrient agar solid medium, activate at 35°C for 24 hours;

[0043] 2) Pick a ring of sludge from the activated Microbacterium X-26, Bacillus X-28 and Microbacterium X-18 with an inoculation loop and add them to the LB liquid medium respectively. 22 into the NA liquid medium, 35 ° C, 200r / min constant temperature shaking for 24 hours to prepare the seed liquid; the pH value of the seed liquid changes are shown in Table 2;

[0044] Table 2 pH change results before and after seed solution

[0045] pH before culture pH after incubation culture medium Microbacterium sp. X-18 6.8-7.0 4.78 LB Koukia X-22 6.8-7.0 8.35 NA Microbacterium sp. X-26 6.8-7.0 8.77 LB Bacillus X-28 6.8-7.0 8.39 LB

[0046] 3) Take the seed liquid according to the inoculation amount of 3% and ino...

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Abstract

The invention discloses a method for efficiently and rapidly retrieving green and improving soil pH value by spray-seeding robinia pseudoacacia on the exposed rock wall of shale, and belongs to the technical field of ecological restoration and soil improvement. Mixed microorganisms, an organic fertilizer and soil are spray-seeded onto the exposed rock wall of the shale through a borrowed soil spray-seeding method, the green-covered plant is robinia pseudoacacia, and the purposes of efficiently and rapidly retrieving green and improving the soil pH value are achieved. The mixed microorganisms are composed of Kocuria X-22, microbacterium X-26, bacillus X-28, and microbacterium X-18, and the mixed microorganisms are added into the organic fertilizer and the soil with a fermentation bacterialsolution. The weight ratio of the mixed microorganisms to the organic fertilizer to the soil is 1:1:8. By means of the method, synergism of microbial flora and leguminous nitrogen-fixing bacteria is achieved, rapid growth of the robinia pseudoacacia on the exposed rock wall of the shale is promoted, the robinia pseudoacacia soil organic matter content, available phosphorus content and pH value canbe significantly improved, the soil fertility is improved, the application dose of the chemical fertilizer is reduced, and a low-cost efficient new method can be provided for efficient and rapid green retrieving of the exposed rock wall of the shale.

Description

technical field [0001] The invention belongs to the technical field of soil improvement, and in particular relates to a method for spraying black locust on exposed rock walls of shale for efficient and rapid regreening and improving soil pH. Background technique [0002] Soil is the foundation of forestry production, an important carrier of nutrients in material cycle and energy flow, and one of the important factors affecting the environment. With the advancement of human civilization, a series of activities such as mining and road construction have caused ecological problems such as the destruction of mountain vegetation, exposed rock walls, and increasing soil erosion. Human development and utilization of land will not only change the soil structure, but also change the soil fertility and activity, etc., which has a serious impact on the cycle of soil materials. [0003] Soil acidity and alkalinity are important indicators for evaluating soil nutrients, and have extremel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01B79/02C09K17/40C09K109/00C09K105/00C09K101/00
CPCA01B79/02C09K17/40C09K2109/00C09K2101/00C09K2105/00
Inventor 庄家尧刘超凡国华田琨张金池
Owner NANJING FORESTRY UNIV
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