Sperm sorting device

A sorting device and sperm technology, which is applied in the interdisciplinary field of micro-nano processing and life sciences, can solve the problems of uneven hole size, low porosity, and affecting the sorting effect of sperm sorting devices, and achieve improved sorting efficiency effect

Active Publication Date: 2019-12-10
合肥晶优生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the preparation of commercial polycarbonate filter membranes used in traditional sperm sorting devices is poorly controllable, not only the pore size is not uniform, the pore distribution is uneven, and the porosity is very low, which seriously affects the sorting effect of the sperm sorting device

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] The preparation of polydimethylsiloxane (PDMS) through-hole membrane comprises the following steps:

[0070] First, the S1813 photoresist was spin-coated on the silicon wafer substrate, the spin-coating speed was 3000rpm, the spin-coating time was 40s, and the spin-coating thickness was 1 μm. Then, it was baked and cured at 115° C. for 90 s. Afterwards, a UV lithography machine (SUSS MA6) is used for pan exposure, and the pan exposure time is 9s, and a photoresist layer with sufficient chain scission can be obtained.

[0071] The prepared photoresist layer is subjected to surface O in a glue remover. 2 Plasma treatment, O 2 The flow rate is 30sccm, the radio frequency power is 30w, and the processing time is 2min. Then, spin-coat the PDMS film layer on the surface of the treated photoresist layer, wherein the PDMS glue solution that is spin-coated is SYLGARD 184 produced by Dow Corning. This product is a package product composed of PDMS prepolymer and curing agent. ...

Embodiment 2

[0077] (1) Sperm sorting device

[0078] This embodiment provides a figure 1 The sperm sorting device with the structure shown includes: a semen inflow channel 2 and a semen cavity. Wherein, the semen cavity is divided into upper and lower two chambers by the horizontally arranged polydimethylsiloxane through-hole membrane 4 (made in Example 1), the lower chamber is the semen storage chamber 3, and the upper chamber is the semen storage chamber 3. Semen recovery chamber 5 ; one end opening of the semen inflow channel 2 serves as the semen inlet 1 , and the other end opening communicates with the semen storage chamber 3 .

[0079] In this embodiment, the semen inlet 1, the semen inflow channel 2 and the semen storage chamber 3 are located in the first polymer layer 1-2, and the material of the first polymer layer 1-2 is polydimethylsiloxane; semen recovery The chamber 5 is located in the second polymer layer 1-3, and the material of the second polymer layer 1-3 is polydimethy...

Embodiment 3

[0087] Chemotactic sperm sorting device

[0088] This embodiment provides a figure 2 and image 3 The sperm sorting device with the structure shown includes: a semen inflow channel 2, a semen cavity, a first chemotaxis sorting channel 6-1 and a chemotaxis sorting second channel 6-2. Wherein, the semen cavity is divided into upper and lower two chambers by the horizontally arranged polydimethylsiloxane through-hole membrane 4 (made in Example 1), the lower chamber is the semen storage chamber 3, and the upper chamber is the semen storage chamber 3. Semen recovery chamber 5; one end opening of semen inflow channel 2 serves as semen inlet 1, and the other end opening communicates with semen storage chamber 3; chemotaxis sorting first channel 6-1 and chemotaxis sorting second channel The opening at one end of 6-2 communicates with the semen recovery chamber 5, and the opening at the other end serves as the exit 6 of the chemotaxis sorting channel; cumulus cells are cultivated i...

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Abstract

The invention belongs to the crossing field of combining micro-nano machining with life science and particularly relates to a sperm sorting device. The sperm sorting device provided by the invention comprises a semen cavity and a semen inflow channel communicating with a semen storage cavity; the semen cavity is divided into an upper cavity and a lower cavity by a horizontally arranged PDMS through hole membrane; the lower cavity is the semen storage cavity; the upper cavity is a semen recovery cavity; and the PDMS through hole membrane is prepared by micro-nano machining. For the sperm sorting device provided by the invention, a filter membrane adopted by the sperm sorting device is the PDMS through hole membrane prepared by a micro-nano machining technology, and the thickness, the pore diameter, the uniformity and the porosity of the PDMS through hole membrane prepared by the method are highly controllable, so that the sperm sorting device provided by the invention can realize greatimprovement on the sperm recovery efficiency through a mode of increasing the porosity of the arranged PDMS through hole membrane.

Description

technical field [0001] The invention belongs to the intersecting field of combining micro-nano processing and life sciences, and in particular relates to a sperm sorting device. Background technique [0002] According to the 2010 "China Infertility Status Survey Report", in China, the incidence rates of infertility for men and women for one year, two years and ten years are 10%, 15% and 25% respectively. It is already close to the average infertility rate of 15% to 20% in developed countries, and infertile couples tend to be younger. The youngest age for consultation is 23 years old, and the oldest age is 40 years old. The main cause of male infertility is low sperm count, often associated with low sperm motility and impaired sperm function, resulting in an inability to fertilize oocytes naturally. Of all the assisted reproductive technologies (ARTs) available, intraplasmic sperm injection (ICSI) has emerged as a powerful tool in the management of male factor infertility. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/12
CPCC12M47/04
Inventor 周成刚陈陈魏珊珊侯小虎龙世兵
Owner 合肥晶优生物科技有限公司
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