Extraction method of yak achilles tendon bone collagen
A technology of bone collagen and extraction method, which is applied in the field of extraction of beef Achilles tendon bone collagen, can solve problems such as inconvenient cleaning, low enzymatic hydrolysis efficiency, long enzymatic hydrolysis time, etc., to ensure quality and yield, and extract process Simple and cost-effective extraction
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[0029] Example 1
[0030] A method for extracting yak Achilles tendon bone collagen, comprising the following steps:
[0031] (1) Pretreatment of yak Achilles tendon: Cut fresh yak Achilles tendon into slices with a thickness of 5-10 mm, then put them in 0.7-0.9 mol / L salt water, stir and soak for 23-25 hours, and then put them in Soak in warm water at 42~46℃ for 12~15h, change the water every 30~40min, add 3.9~4.1% hydrochloric acid for 10~12h, replace the hydrochloric acid solution every 100~110min, take out and wash with drinking water 5~6 times , Naturally air-dried; the Achilles tendon of the yak is the Achilles tendon of the mature yak grown in the grasslands of Xinjiang, Hulunbuir, Inner Mongolia, Tibet or Qilian Mountains;
[0032] (2) Enzymatic hydrolysis: adding trypsin to the bovine Achilles tendon treated in step (1), inactivating the enzyme at 65-70° C. after microwave treatment, then adding pepsin, and ultrasonically treating to obtain an enzymatic hydrolysis so...
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[0039] Example 2
[0040] The difference from Example 1 is that: in step (2), the amount of trypsin added is 0.25% of the weight of the yak achilles tendon; the enzymatic hydrolysis conditions of the trypsin are: the pH value is 6.9, and the reaction temperature is 60 ℃, the enzymatic hydrolysis time is 4h, and other conditions remain unchanged.
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[0041] Example 3
[0042] The difference from Example 1 is that: in step (2), the frequency of the microwave treatment is 0.35KHz, the power is 400W, the time is 110min, and other conditions remain unchanged.
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