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Extraction method of yak achilles tendon bone collagen

A technology of bone collagen and extraction method, which is applied in the field of extraction of beef Achilles tendon bone collagen, can solve problems such as inconvenient cleaning, low enzymatic hydrolysis efficiency, long enzymatic hydrolysis time, etc., to ensure quality and yield, and extract process Simple and cost-effective extraction

Inactive Publication Date: 2019-12-13
NANNING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0009] However, the above disclosed patent application documents have problems and shortcomings: low enzymolysis efficiency, long enzymolysis time, large amount of enzyme addition, complicated operation, high energy consumption, and organic solvents such as ethanol used in the extraction process. There will be organic solvent residues, and it is inconvenient to clean, the elution is difficult, and the extraction rate is low

Method used

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  • Extraction method of yak achilles tendon bone collagen
  • Extraction method of yak achilles tendon bone collagen

Examples

Experimental program
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Effect test

Embodiment 1

[0030] A method for extracting collagen from beef Achilles tendon, comprising the following steps:

[0031] (1) Pretreatment of Achilles tendon: Cut fresh Achilles tendon into slices with a thickness of 5-10mm, put it into 0.7-0.9mol / L salt water, stir and soak for 23-25 ​​hours, and then put it into Soak in warm water at 42-46°C for 12-15 hours, change the water every 30-40 minutes, add 3.9-4.1% hydrochloric acid to soak for 10-12 hours, replace the hydrochloric acid solution every 100-110 minutes, take it out and wash it with drinking water 5-6 times , naturally air-dried; the Achilles tendon of the beef cattle is the Achilles tendon of mature cattle that grow in Xinjiang grassland, Hulunbeier grassland, Inner Mongolia grassland, Tibet grassland or Qilian Mountain grassland;

[0032] (2) Enzymolysis: add trypsin to the bovine Achilles tendon treated in step (1), inactivate the enzyme at 65-70° C. after microwave treatment, then add pepsin, and perform ultrasonic treatment to...

Embodiment 2

[0040] The difference from Example 1 is that in step (2), the amount of trypsin added is 0.25% of the weight of the Achilles tendon consumed; the enzymolysis conditions of the trypsin are: the pH value is 6.9, and the reaction temperature is 60 ℃, the enzymatic hydrolysis time is 4h, and other conditions remain unchanged.

Embodiment 3

[0042] The difference from Example 1 is that in step (2), the frequency of the microwave treatment is 0.35KHz, the power is 400W, the time is 110min, and other conditions remain unchanged.

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Abstract

The present invention discloses an extraction method of yak achilles tendon bone collagen. The extraction method of the yak achilles tendon bone collagen comprises the following steps: (1) conductingpretreatment of yak achilles tendon: cutting fresh yak achilles tendon into slices, soaking in edible salt water, soaking in warm water, soaking in hydrochloric acid, conducting taking out and washing, conducting natural air-drying, adding trypsin, inactivating enzymes after a microwave treatment, then adding pepsin, conducting an ultrasonic treatment, conducting centrifugal filtration, collectinga filtrate, conducting salting-out dialysis on the filtrate, and conducting freeze-drying to obtain the yak achilles tendon bone collagen. A degreasing and decalcification treatment before enzymolysis is conducted to improve enzyme activity, besides, microwave and ultrasonic wave assisting enzymolysis are combined to improve solubility of the bone collagen, so that an extraction rate of the bonecollagen is improved. In addition, no organic solvent is added in an extraction process, organic solvent residues exist in the product, quality and yield of the bone collagen are ensured, besides, theextraction technology is simple, simple and convenient for operation, and low in energy consumption, and saves extraction cost.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and in particular relates to a method for extracting collagen from beef Achilles tendon. Background technique [0002] Collagen is a kind of protein family with its special triple helix structure. At least 30 kinds of coding genes of collagen chains have been found, which can form more than 16 kinds of collagen molecules. According to their structure, they can be divided into fibrous collagen, Basement membrane collagen, microfibril collagen, anchor collagen, hexagonal network collagen, non-fibrous collagen, transmembrane collagen, etc. According to their distribution and functional characteristics in the body, collagen can be divided into interstitial collagen, basement membrane collagen and peripheral collagen. Interstitial collagen molecules account for the vast majority of the collagen in the whole body, including type I, II, and III collagen molecules. Type I collagen is mainly...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/34
CPCC07K14/78C12P21/06
Inventor 谭春萍韦平黄广君黄春兰
Owner NANNING UNIV
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