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Aseptic seedling inducing method for saraca dives pierre

A technology of worry-free flowers and sterile seedlings, applied in horticultural methods, botanical equipment and methods, horticulture and other directions, can solve the problems of serious browning, difficulty in germination, inconvenient tissue culture operation, etc., to improve antioxidant problems, The effect of reducing the pollution rate and reducing the browning rate

Active Publication Date: 2019-12-17
BEIBU GULF UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is also mentioned in the prior art Chinese patent CN 108323425A "A Cutting Propagation Method of Flos Violet Flos., that at present, the tissue culture and propagation of Flos. Flos.
[0003] In the actual tissue culture technology research, it is found that, similar to most woody plants, using the stems of S. chinensis as explants has a relatively serious browning problem, and it is not easy to induce sterile buds
Using the seeds of Chinese worry-free flower for aseptic sowing, there is also browning phenomenon, and it is difficult to germinate
At the same time, due to its large size, the length and width are about 4.1cm×2.5cm, which is inconvenient for tissue culture operation

Method used

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  • Aseptic seedling inducing method for saraca dives pierre
  • Aseptic seedling inducing method for saraca dives pierre

Examples

Experimental program
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Effect test

Embodiment 1

[0032] A method for aseptically inducing S. japonica, said method comprising the steps of:

[0033] (1) Collection and processing of fruit pods: Before the seeds are fully mature, about mid-to-late June, pick uncracked, plump, and free of diseases and insect pests;

[0034] (2) Low-temperature treatment: place the fruit pods obtained in step (1) in a 5°C refrigerator for 4 days and set aside;

[0035] (3) Disinfection of seeds: Peel off the fruit pods, select smooth and relatively plump seeds, and disinfect the seeds with disinfectant on the ultra-clean workbench; wherein, the disinfectant disinfection method is: soak the seeds in Put it in the disinfectant solution I for 15 minutes; take it out and soak it in the disinfectant solution II for 30 seconds; take it out and rinse it with sterile water for 3 times. Among them, the disinfectant solution I is HgCl with a mass percentage of 0.1%. 2 and 0.01% Tween-80 solution mixture; Disinfectant II is a 75% ethanol solution by vol...

Embodiment 2

[0041] A method for aseptically inducing S. japonica, said method comprising the steps of:

[0042] (1) Collection and processing of fruit pods: Before the seeds are fully mature, about mid-to-late June, pick uncracked, plump, and free of diseases and insect pests;

[0043] (2) Low-temperature treatment: place the fruit pods obtained in step (1) in a 5°C refrigerator for 7 days and set aside;

[0044] (3) Disinfection of seeds: Peel off the fruit pods, select smooth and relatively plump seeds, and disinfect the seeds with disinfectant on the ultra-clean workbench; wherein, the disinfectant disinfection method is: soak the seeds in Put it in the disinfectant solution Ⅰ for 20 minutes; take it out and soak it in the disinfectant solution Ⅱ for 30 seconds; take it out and rinse it with sterile water for 3-5 times. Among them, the disinfectant solution I is HgCl with a mass percentage of 0.1%. 2 and 0.01% Tween-80 solution mixture; Disinfectant II is a 75% ethanol solution by vo...

Embodiment 3

[0050] A method for aseptically inducing S. japonica, said method comprising the steps of:

[0051] (1) Collection and processing of fruit pods: Before the seeds are fully mature, about mid-to-late June, pick uncracked, plump, and free of diseases and insect pests;

[0052] (2) Low-temperature treatment: place the fruit pods obtained in step (1) in a 5°C refrigerator for 5 days and set aside;

[0053] (3) Disinfection of seeds: Peel off the fruit pods, select smooth and relatively plump seeds, and disinfect the seeds with disinfectant on the ultra-clean workbench; wherein, the disinfectant disinfection method is: soak the seeds in Put it in the disinfectant solution Ⅰ for 17 minutes; take it out and soak it in the disinfectant solution Ⅱ for 30 seconds; take it out and rinse it with sterile water 4 times. Among them, the disinfectant solution I is HgCl with a mass percentage of 0.1%. 2 and 0.01% Tween-80 solution mixture; Disinfectant II is a 75% ethanol solution by volume; ...

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Abstract

The invention relates to the technical field of saraca dives pierre cultivation, in particular to an aseptic seedling inducing method for saraca dives pierre. According to the aseptic seedling inducing method, an embryo formed by natural pollination near the mature stage is adopted for embryo culture to obtain an aseptic seedling, so that a method and basis are provided for establishing a completetissue culture and rapid propagation technology system for the saraca dives pierre. For the own characteristics of the saraca dives pierre, firstly determining the seed collecting time is the key link. An unbroken saraca dives pierre capsule near the mature stage is collected, the capsule is stripped off to select a healthy seed as a disinfection material, and a young embryo is taken under an aseptic condition for culture, wherein the disinfection success rate can reach 90% or above; according to the characteristic that a saraca dives pierre tissue material is easy to brown and not easy to induce, through low-temperature treatment on the capsule, browning can be reduced, and young embryo germination can be promoted; by adding an antioxidant into a culture medium, and optimizing and improving the species selection, use amount and use method, the tissue culture seedling with a lower browning rate and a higher seedling rate is obtained.

Description

【Technical field】 [0001] The invention relates to the technical field of cultivating saffron, in particular to a method for inducing aseptic seedlings of saffron. 【Background technique】 [0002] Worry-free flower refers to Chinese worry-free flower (scientific name: Saraca dives Pierre), also known as flame flower. It is an evergreen tree with dense branches and leaves, large and red flowers, and it looks like flames in the distance when it is in full bloom. It is a plant with great prospects for garden development and application; The result is not easy to get the seeds. At present, methods such as sowing, cuttings and high-altitude layering can be used for the propagation of saffron. Among them, sowing is the most commonly used method of propagation. However, according to reports, although the worry-free flowers planted in Guangzhou and neighboring areas grow well and can bloom and bear fruit, due to the serious damage of Cryptophlebia ombrodelta Lower during the pod matu...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/00
Inventor 王华宇杨利平何贵整王鹏良陈乃明陈丽文陈俊锦杨琼陈乃健
Owner BEIBU GULF UNIV