Composite microbial fertilizer capable of being stored for long time, and preparation method thereof
A technology of compound microorganisms and fertilizers, which is applied in the field of compound microbial fertilizers and its preparation, can solve the problems of low viable bacteria rate, high rate of miscellaneous bacteria, mildew and other problems of microbial fertilizers, and achieve long-term preservation, increase the number of bacteria, and improve survival rate effect
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Embodiment 1
[0019] Preparation of mixed bacterial powder of Bacillus megaterium and Bacillus jelly: The Bacillus megaterium used in the present invention is ordered from the China Agricultural Microorganism Preservation Management Center, and the preservation number is ACCC02991; The number is ACCC02983. Inoculate the purchased Bacillus megaterium and Bacillus jellyon respectively on LB solid medium, and after culturing at 37°C for 12 hours, inoculate single plaques into LB liquid medium, and culture them on a shaker at 37°C for 24 hours. After 1 hour, the two bacterial solutions were mixed evenly at a volume ratio of 1:1. Inoculate the mixed bacterial solution into a fermenter, and cultivate it at 37°C for 12-16 hours, during which sterile air is continuously introduced. The liquid medium includes 100L sterilized water, 2Kg glucose, 1.5Kg peptone, 0.5Kg chloride Sodium, 0.05Kg beef extract, pH is 7.0~7.5. After the fermentation is over, add starch equivalent to 5% of the fermentation b...
Embodiment 2
[0026] Get 5 groups of fertilizers (experimental groups) prepared by Example 1 and store them in a cool and ventilated place at low temperature, and get 5 groups of newly prepared common compound microbial fertilizers (control groups) and store them in the same place. After 365 days, 540 days and 730 days of storage, the number of live bacteria in the fertilizer was detected and the average value was calculated. The specific detection method is as follows:
[0027] 1) Weigh 1g of organic particles in the fertilizer, break the outer layer formed by the fermentation product, disperse the biochar particles in the protective agent (5% starch + 2% dextrin), stir at 200rpm for 15min, and pick out the biochar particles , keep the bacterial suspension;
[0028] 2) Stir the bacterial suspension in a water bath at 75°C for 20 minutes, then rapidly cool to 37°C to obtain a sterilized suspension;
[0029] 3) Take 1ml of sterile suspension and dilute 10 6 After that, inoculate to 10cm ...
Embodiment 3
[0033] Get the fertilizer (experimental group) that makes according to embodiment 1 and preserve in the cool and ventilated place of low temperature, get the common compound microbial fertilizer (control group) that makes newly similarly and preserve in the same place. Observe the moldiness of the fertilizer after 365 days, 540 days and 730 days of storage respectively. For each observation, randomly take 5 groups of fertilizers in the bags of the experimental group and the control group, 5g in each group, detect the number of molds in the 5g fertilizer, and take the average value of the 5 groups and record it.
[0034]
[0035] According to the experimental results, it can be seen that the compound microbial fertilizer of the present invention uses lemonade and turmeric which have the effect of killing fungi. After two years of storage of the fertilizer, the number of molds is still very little and far less than that of the control group, which is suitable for long-term pre...
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