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Method for producing protein feed through different mixed fermentation twice of fine-brewed brewer's grains and blueberry fruit residues

A blueberry pomace, protein feed technology, applied in animal feed, animal feed, application and other directions, can solve the problems of insufficient cellulose degradation, affecting the protein production rate, etc., to reduce resource waste and environmental pollution, increase significantly, reproduce strong effect

Pending Publication Date: 2019-12-20
EASTERN LIAONING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In view of the high cellulose content of craft brewer's grains and blueberry pomace, if the original process is used, the degradation of cellulose will not be enough, which will affect the protein production rate

Method used

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  • Method for producing protein feed through different mixed fermentation twice of fine-brewed brewer's grains and blueberry fruit residues
  • Method for producing protein feed through different mixed fermentation twice of fine-brewed brewer's grains and blueberry fruit residues
  • Method for producing protein feed through different mixed fermentation twice of fine-brewed brewer's grains and blueberry fruit residues

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] 1. First test the crude fiber content of the mixture of craft brewer's grains, blueberry pomace and bran (the ratio of craft brewer's grains to blueberry pomace is 1:1, and the ratio of the mixture to bran is 4:1)

[0044] Test method: Weigh 2g of the sample, degrease it with ether, put it into a digester, add 200ml of boiling sulfuric acid solution with an accurate concentration of 0.255N and 1 drop of n-octanol, heat immediately, and make it boil within 2min. And continue to slightly boil for 30±1min, pay attention to keep the concentration of sulfuric acid unchanged, then filter, wash with boiling distilled water until no acid is present, remove the insoluble matter, put it into the original container, add 200ml of boiling sodium hydroxide solution with accurate concentration, Also accurately boil for 30 minutes. Immediately filter with asbestos on a Gooch's crucible, first wash with 25ml of sulfuric acid solution, then use boiling distilled water, wash until the lot...

Embodiment 2

[0081] (1) Strains and sources

[0082] Common high-temperature actinomycetes (strain preservation number: CICC 10672, Latin name: Thermoactinomycesvulgaris), Geotrichum candidum (strain preservation number: CICC 1443 Latin name: Geotrichum candidum) and Lactobacillus plantarum (strain preservation number: CICC 21794 Latin name: Lactobacillus plantarum ) were purchased from the China Industrial Microbiology Culture Collection Management Center.

[0083] (2) Activation and preparation of strains

[0084] Original seed test tube activation-triangular flask expansion-seed tank culture-fermenter tank culture

[0085] Ordinary actinomycetes culture medium is glucose 4.0g, yeast extract 4.0g, malt extract 10.0g, CaCO 3 2.0g, 12.0g agar, 1.0L distilled water, pH7.2, the prepared slant medium should be sterilized at 121°C for 20min before use; just remove the agar when making liquid medium.

[0086] Geotrichum candidum medium is 5°Bé malt juice agar medium, and its preparation ste...

Embodiment 3

[0098] (1) strain and source: same as embodiment 2

[0099] (2) Activation and preparation of strains

[0100] Geotrichum candidum and Lactobacillus plantarum are mixed in 4:1 ratio, and others are identical with embodiment 2.

[0101] (3) Preparation of fermentation raw materials: 51.4% of fine brewer's grains, 36.2% of blueberry pomace, 9.9% of bran, and the inoculation amount of mixed bacteria of Geotrichum candidum and Lactobacillus plantarum was 5%.

[0102] (4) Strain inoculation and preparation process

[0103] Firstly, the inoculum amount of ordinary high-temperature actinomycetes is 10% of the weight of the fermentation raw material, and the sterilized bran is carried out by conventional carrier technology to make solid mixed bacterial agent II, and then Geotrichum candidum and plant milk with a volume ratio of 4:1 The inoculum amount of bacilli according to the weight of 5% of the fermentation raw material is sterilized bran and carried by conventional carrier tech...

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Abstract

The invention discloses a method for producing a protein feed through different mixed fermentation twice of fine-brewed brewer's grains and blueberry fruit residues, and belongs to the technical fieldof the production of protein feeds. The method specifically comprises the steps of respectively performing protospecies test tube activation, triangular flask enlarged culture and seeding tank culture on ordinary thermoactinomyces, geotrichum candidum and lactobacillus plantarum, enabling a fermenter to be subjected to standing so as to obtain bacterium suspension, and mixing cultured strains inproportion so as to obtain mixed bacteria; preparing fermentation raw materials of the fine-brewed brewer's grains, the blueberry fruit residues and bran; preparing the ordinary thermoactinomyces, thegeotrichum candidum and the lactobacillus plantarum according to a certain inoculum concentration into a solid mixed bacterium agent through sterilized bran; and mixing the prepared solid mixed bacterium agent with the fine-brewed brewer's grains and the blueberry fruit residues, performing solid fermentation, firstly performing inoculation with the ordinary thermoactinomyces, performing culturefermentation at constant temperature of 50 DEG C for the first time for 72h, then performing inoculation with the obtained geotrichum candidum and lactobacillus plantarum mixed bacteria, performing fermentation at 30 DEG C for the second time for 72h, drying fermented products, and performing crushing so as to obtain the protein feed.

Description

technical field [0001] The invention belongs to the technical field of production of protein feed, and in particular relates to a method for producing protein feed by performing two different mixed fermentations by using refined brewer's grains and blueberry pomace. Background technique [0002] my country is a large animal husbandry and feed processing country, and feed accounts for more than 70% of the cost of breeding. According to the statistics of the China Business Industry Research Institute, my country's feed production in 2017 was 284.655 million tons, and the import rate of feed raw materials, especially protein feed raw materials, was close to 80%. , has become an important bottleneck restricting my country's aquaculture and feed processing industries. [0003] Against the background that popular beer is becoming increasingly saturated, more and more people are optimistic about China's craft beer market. China's beer market is showing a high-end development trend,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/12A23K10/38A23K10/37
CPCA23K10/12A23K10/38A23K10/37Y02P60/87
Inventor 宋宇
Owner EASTERN LIAONING UNIV
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