Low-sugar probiotic composition as well as preparation method and application thereof

A composition and probiotic technology, applied in the direction of food science, etc., can solve problems such as flatulence and neglect, and achieve the effect of reducing the possibility of flatulence

Inactive Publication Date: 2019-12-20
ZHEJIANG HUAKANG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The latest definition of prebiotics is a substrate selectively utilized by host microorganisms that confers health benefits on the host, such as common fructans and galactans, but intestinal microbial fermentation produces gas, which may cause flatu...

Method used

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  • Low-sugar probiotic composition as well as preparation method and application thereof
  • Low-sugar probiotic composition as well as preparation method and application thereof
  • Low-sugar probiotic composition as well as preparation method and application thereof

Examples

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preparation example Construction

[0028] The present invention also discloses a method for preparing the above-mentioned low-sugar probiotic composition, which includes the following steps:

[0029] Step 1. Dissolve steviol glycosides in formic acid solution, stir the reaction, concentrate to remove the reaction solvent, wash the obtained precipitate with ultrapure water, filter with suction, concentrate the filtrate again, dissolve it with ultrapure water, keep the supernatant, and prepare the obtained The solution is separated by a diatom-activated carbon chromatography column, followed by gradient elution with ultrapure water and ethanol solution to obtain a separated and purified sophorose solution.

[0030] Step 2. Spray-dry or freeze-dry the above-mentioned separated and purified sophorose solution to obtain sophorose powder.

[0031] Step 3: uniformly mix the prepared sophorose powder with xylitol, L-arabinose and dietary fiber, and ultrafinely pulverize to obtain the composition.

[0032] The inventio...

Embodiment 1

[0037] The first preparation method of the present invention for preparing a low-sugar probiotic composition comprises the following steps:

[0038] 11. Preparation of sophorose solution

[0039] Dissolve 10g steviol glycoside (98%) in 20~80mL formic acid solution, stir and react at 80°C for 30~40min, remove the reaction solvent by vacuum rotary evaporation, wash the obtained precipitate with 100mL ultrapure water, suction filter, discard the precipitate, Rotate again to obtain a second spin-evaporation solution, dissolve it in 100ml ultrapure water, and keep the supernatant. The obtained solution is separated by a diatom-activated carbon chromatography column, followed by gradient elution with ultrapure water and 5-30% ethanol solution to obtain a separated and purified sophorose solution.

[0040] Compared figure 1 , figure 2 and image 3 The chromatogram shows that the concentration of sophorose in the reaction solution obtained by preparing sophorose by this method is...

Embodiment 2

[0061] The second preparation method of the present invention for preparing a low-sugar probiotic composition comprises the following steps:

[0062] Step 21, preparation of sophorose solution, and step 22, preparation of sophorose are the same as step 11 and step 12 of Example 1 respectively, and will not be repeated here.

[0063] 23. Preparation of probiotic composition

[0064] The sophorose solution separated and purified above is subjected to centrifugal spray drying to obtain the sophorose powder. The drying conditions are set at an inlet temperature of 160-180°C and an outlet temperature of 60-80°C, and the yield of sophorose reaches 30%-40%.

[0065] Each component was weighed according to the following formula: 35% sophorose, 20% xylitol, 20% L-arabinose and 25% dietary fiber, mixed evenly, and ultrafinely pulverized to obtain the composition.

[0066] The low-sugar composition prepared in this embodiment was subjected to the same evaluation experiment as in Exampl...

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Abstract

The invention relates to a low-sugar probiotic composition comprising sophorose, xylitol, L-arabinose and dietary fiber. The invention also discloses a preparation method and application of the low-sugar probiotic composition. The low-sugar probiotic composition has the effect of promoting proliferation of intestinal beneficial bacteria or improving metabolic activity, and can induce intestinal microorganisms to secret cellulase and reduce the probability of flatulence.

Description

technical field [0001] The invention relates to the technical field of prebiotic compositions, in particular to a low-sugar prebiotic composition and its preparation method and application. Background technique [0002] Sophorose (sophorose, 2-O-β-D-glucopyranosyl-α-D-glucose, C12H22O11, MW 342.29), is a kind of two glucose units connected by glycosidic bond β (1→2) Disaccharide, which is an important component of antibacterial and amphiphilic biosurfactant sophorolipids, is the product of glucose caramelization, mainly exists in a small amount in the Sophora japonica of Sophora japonica in nature, and was isolated for the first time in 1938 come out. [0003] Mäkeläinen H et al. have found that sophorose can promote the growth of beneficial bacteria (Bifidobacterium lactis Bi-07, Lactobacillus acidophilus NCFM, Lactobacillus rhamnosus HN001), but cannot be utilized by pathogenic bacteria (Salmonella typhimurium, Clostridium perfringens). According to the existing in vitro...

Claims

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Application Information

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IPC IPC(8): A23L33/135A23L33/125A23L33/21
CPCA23L33/135A23L33/125A23L33/21A23V2002/00
Inventor 石丽华陈德水朱炫左齐乐张文瑶李勉
Owner ZHEJIANG HUAKANG PHARMA
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