Culture medium for separating fungi from yeast-like fungi and preparation method

Abstract

The invention relates to the technical field of medical chemiluminescence immunoassay detection, and particularly relates to a culture medium for separating fungi from yeast-like fungi and a preparation method thereof. Casein peptone, animal peptone and glucose are used for providing a proper carbon source and a proper nitrogen source for microorganisms, the ratio of the whole carbon source to thewhole nitrogen source is controllable, meanwhile, the content of the glucose is higher, the pH value of the whole culture medium tends to 5.5-6.5, and growth of fungi and yeast-like fungi is facilitated. Meanwhile, single chloramphenicol is adopted as a bacterial inhibitor to effectively inhibit the growth of bacteria, and to reduce the probability that part of to-be-separated fungi are inhibiteddue to cooperation of multiple bacteriostatic agents. The invention solves the problems that: an existing culture medium for separating fungi is single in carbon source and nitrogen source component,the imbalance of the ratio of a carbon source to a nitrogen source is likely to occur, meanwhile, multiple bacteriostatic agents are combined for bacteriostasis, the bacteriostatic effect on part offungi is overlapped, and the effect of separating part of fungi is poor.

Description

technical field [0001] The invention relates to the technical field of medical chemiluminescence immunoassay detection, in particular to a medium for separating fungi and yeast-like fungi and a preparation method. Background technique [0002] Candida is an opportunistic pathogenic bacteria that exists in the skin, oral cavity, vagina, intestinal mucosa, etc. When the body's immunity is low, it leads to common clinical infections and related diseases. In order to be able to accurately diagnose the type of bacteria at the site of infection, clinically, samples are often collected from the patient’s site for culture, and clinical examiners select suspicious strains for identification based on clinical symptoms, so as to determine the type of bacterial infection at the patient’s site, providing a basis for clinical treatment in accordance with. For the diagnosis of the type of bacteria infected at the patient site, culture is a feasible method in clinical diagnosis at present....

AI Technical Summary

Problems solved by technology

[0004] The present invention aims at the single carbon source and nitrogen source components in the culture medium for separating fungi, which is prone to imbalance in the ratio of source and nitrogen source. At the same time, a combination of various antibacterial agents is used to inhibit bacteria, so that the antibacterial effect is superimposed on some fungi. phenomenon, which leads to the problem of poor effect of separating some fungi, and provides a culture medium and preparation method for isolating fungi and yeast-like fungi