Application and fixed-point knockout method of EMC3 gene

Abstract

The invention belongs to the biology field, and particularly relates to application and a fixed-point knockout method of an EMC3 gene. Through targeted modification of the EMC3 gene by a gene editingtechnology and change of basic groups of a coding region to cause frame shift mutation of the EMC3 gene, an EMC3 gene knockout cell line is obtained. Experiments prove that by changing a nucleotide sequence of the EMC3 gene in a swine kidney cell (PK-15) to delete EMC3 protein expression, and proliferation of Japanese encephalitis virus (JEV) can be interfered significantly, so that host cells canbe effectively protected against JEV infection invasion induced death. Multiple sequence alignment analysis finds that the EMC3 gene sequence is highly conservative in pigs, people and mice. Therefore, the EMC3 gene can be used as a gene editing target for resisting Japanese encephalitis or used for developing anti-JEV drugs.

Description

technical field

[0001] The invention belongs to the field of biotechnology, and in particular relates to the application of EMC3 gene and its fixed-point knockout method. Background technique

[0002] Various infectious diseases caused by pathogenic microorganisms have always been a major obstacle to the efficient development of the global pig industry. The economic loss of pig farming caused by infectious diseases in China is estimated to be as high as 40 billion yuan every year. Especially since 2018, African swine fever has been introduced into China, which has severely damaged the domestic pig industry. China is the world's largest pig breeding and pork consumer. The epidemic not only affects the pig industry, but also affects related international trade. How to effectively prevent and control the occurrence of diseases has always been the focus of animal husbandry. Although vaccination has played an important role in prevention and control, it has not been able to com...

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