A Tissue Culture Rapid Propagation Method of Orchid and Its Application

Abstract

The invention relates to the technical field of plant tissue culture, and discloses a tissue culture and rapid propagation method for plants of the genus Cymbidium, including the acquisition of sterile explants, induction culture, differentiation culture, rooting culture, hardening and transplanting; wherein, the The induction culture is specifically as follows: take the sterile explant 0.5-2.0 cm, inoculate it in the induction medium, cultivate it for 7-15 days, and obtain the callus; the temperature of the induction culture is 24-28 ° C, and the light time is 8~12h / d, the light intensity is 2000~4000Lx; the induction medium includes 0.8~1.5MS medium, 6‑BA 0.5~1.0mg / L, IAA 0.1~0.3mg / L, 2,4‑D 0.1 ~0.2mg / L, sucrose 25~30g / L, agar 5~7g / L, Vc 0.2~0.4mg / L and nitrosoferricyanide sodium 2.0~6.0mg / L, the pH of described induction medium= 5.6~6.2; the present invention also discloses the application of the tissue culture rapid propagation method, the tissue culture rapid propagation method achieves The effect of high-efficiency breeding of cultivated seedlings of Orchid genus.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a tissue culture and rapid propagation method for plants of the genus Orchid and its application. Background technique [0002] Dracocephalum belongs to Labiatae, herbaceous, perennial, with woody rhizomes, and rarely annual. Stems often arise from rhizomes, erect, sparsely spread, often unbranched or with few branches, sparsely numerous branches, quadrangular. Leaves opposite, basal leaves with long stalks, cauline leaves with short stalks or sessiles, often heart-shaped ovate or oblong, or lanceolate, margins crenate or serrate, or entire, usually not Divided or pinnately nearly palmately parted. Verticels densely clustered into heads or spikes or sparsely arranged; flowers usually blue-purple, rarely white; bracts often obovate, often with sharp teeth or spines, rarely entire. The phytochemical composition of the genus Qinglan is complex, which can be mainly di...

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Problems solved by technology

[0004] However, at present, when conventional plant tissue culture methods and common plant hormone ratios are used to breed the plants of the genus Orchid, the efficiency is very low, even not as high as the seed propagation efficiency

In addition, due to the small size of Maojiancao seeds, it is not easy to collect, and the seed germination rate is not high in the natural environment, which makes it difficult to reproduce the seeds; at the same time, because of its rich secondary metabolites, it contains a variety of enzymes and various small Molecular substances have seriously affected the effect of the phytohormone combination of conventional plant tissue culture, so that the conventional plant tissue culture method can not effectively achieve the efficiency of tissue culture for the breeding of Maojiancao

For example, the commonly used medium for tobacco tissue culture cannot complete the regeneration of plants of the genus Chrysanthemum, such as Minshan Maojiancao, and its induction medium cannot induce enough callus, and the browning of tissue materials is also obvious during the induction process.

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