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Mn<2+>-doped polydopamine nano-carrier and preparation method thereof

A technology of polydopamine nano and polymerized dopamine, which is applied in the field of polydopamine nanocarriers doped with Mn2+ and its preparation, can solve the problems of reducing CDT efficacy, limited drug loading, and increasing the resistance of tumor cells to oxidative stress, etc., to achieve Good biocompatibility, simple operation and mild reaction conditions

Inactive Publication Date: 2019-12-31
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The drug loading capacity of the porous PLGA nanoparticles prepared by the prior art is relatively limited, and because glutathione is overexpressed in tumor cells, and the produced hydroxyl radicals can be eliminated by glutathione, thus greatly increasing the Resistance to oxidative stress and reduced efficacy of CDT

Method used

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  • Mn&lt;2+&gt;-doped polydopamine nano-carrier and preparation method thereof
  • Mn&lt;2+&gt;-doped polydopamine nano-carrier and preparation method thereof
  • Mn&lt;2+&gt;-doped polydopamine nano-carrier and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0032] (1) Dissolve 10 mg of PLGA and 2 mg of vitamin E polyethylene glycol succinate (TPGS) porogen in 1 mL of acetone, add dropwise to 5 mL of ultrapure water under magnetic stirring (1000 rpm), and continue magnetic stirring for 3 h , acetone was removed by vacuum drying to obtain porous PLGA nanoparticles PLGA NPs.

[0033] (2) The PLGA NPs obtained in step (1) were centrifuged, washed with deionized water until the supernatant was clear, and the precipitate was collected. Resuspend with 0.01mol / mL Tris-HCl buffer to make the concentration 1mg / mL, then add dopamine and MnCl in the same concentration ratio 2 , stirred at 1000rpm for 6h, the Mn of the black solution can be obtained 2+ -PLGA@PDA NPs. Under the same conditions, only dopamine was added to obtain PLGA@PDA NPs.

Embodiment 2

[0035] PLGA nanocarriers and doped Mn 2+ The polydopamine nanocarriers were made into solutions with a concentration of 300 μg / mL with ultrapure water, and then their morphology was measured under a transmission electron microscope.

[0036] Transmission electron microscopy of the two micelles as figure 2 shown. doped Mn 2+ The particle size of the polydopamine nanocarrier is 152 ± 5nm, and there is a layer of black coating on the surface of the PLGA nanocarrier, which further proves that polydopamine is successfully polymerized.

Embodiment 3

[0038] PLGA nanocarriers and doped Mn 2+ The polydopamine nanocarriers were made into solutions with a concentration of 200 μg / mL in ultrapure water, and then their water dynamic particle diameters were measured at 25°C.

[0039] The results of measuring the water dynamic particle size of the two nanocarriers at 25°C are as follows: image 3 As shown, the particle size of PLGA NPs is 143 ± 3 nm, and after polydopamine polymerization, the hydrodynamic average diameter of PLGA@PDA NPs is 189 ± 4 nm. The particle size of PLGA@PDANPs increased significantly, which further indicated the successful polymerization of polydopamine.

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Abstract

The invention relates to a Mn<2+>-doped polydopamine nano-carrier and a preparation method thereof. The Mn<2+>-doped polydopamine nano-carrier is obtained by polymerizing dopamine on the surface of PLGA NPs and doping Mn<2+>. The nano-carrier is gathered at a tumor part through an EPR effect, and hydroxyl radicals are generated through a Fenton-like reaction initiated by the Mn<2+>. A foundation is laid for a nano-system for realizing tumor microenvironment response and biomimetic material polydopamine long-acting circulation.

Description

technical field [0001] The invention belongs to the field of chemotherapeutic drug delivery nanocarriers, in particular to a Mn-doped 2+ Polydopamine nanocarrier and preparation method thereof. Background technique [0002] Polylactic-co-glycolic acid (PLGA) is one of the most widely used biodegradable polymers due to its ability to form stable nanoparticles with minimal systemic degradation for drug delivery or biomaterial applications. toxicity. [0003] Polydopamine (PDA) nano drug delivery system has attracted great attention in recent years. Autoxidative polymerization of dopamine under alkaline conditions can adhere to solid surfaces. In addition, its chemical structure also contains catechol and amino groups, which is convenient for subsequent surface modification. In the past decade, PDA has been widely used as a new functional material in many fields, such as new energy, environment and biomedicine, etc. Due to its good biocompatibility, unique chemical structu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/51A61K47/34A61K47/22A61K47/18A61K33/32A61P35/00
CPCA61K9/5123A61K9/5146A61K9/5153A61K33/32A61P35/00
Inventor 朱利民吴梦王海军谢晓田
Owner DONGHUA UNIV
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