Kit for human papilloma virus typing detection

A technology of human papillomavirus and kit, which is applied in the direction of microbe-based methods, microbiological measurement/inspection, microbes, etc., which can solve the problems of low HPV sensitivity, high quality requirements for personnel, and low accuracy, and achieve improved coverage , Improve detection specificity, reduce damage effect
CN110628953AActive Publication Date: 2019-12-31宁波胤瑞生物医学仪器有限责任公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
宁波胤瑞生物医学仪器有限责任公司
Publication Date
2019-12-31

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Abstract

The invention belongs to the technical field of diagnostic reagents for human papilloma and particularly relates to a kit for human papilloma virus typing detection and a detection method. The kit comprises a primer probe premixed solution; the primer probe premixed solution comprises upstream and downstream primers for detecting HPV type 16, 18, 52 and 58, fluorescent probes for the HPV type 16,18, 52 and 58 respectively, upstream and downstream primers for detecting human actb genes and fluorescent probes for detecting the human actb genes. For the kit provided by the invention, by adding degenerate bases in a universal primer, the coverage degree of the HPV of different types is increased, and no missed detection is effectively achieved; by adding a design of locked nucleic acid in each fluorescent probe, the detection specificity is improved; on the premise that the reaction is guaranteed to be efficient and accurate, the pore inlet efficiency of a chip is greatly improved; and byusing hot started DNA polymerase and UDG enzyme, the reaction false positive is reduced.
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Description

technical field

[0001] The invention belongs to the technical field of diagnostic reagents for human papilloma, and in particular relates to a kit and a detection method for typing and detecting human papilloma virus. Background technique

[0002] Human papillomavirus (Human Papilloma Virus, HPV) belongs to the papillomavirus genus, is a virus that specifically infects the skin or mucosal stratified epithelium. HPV is a double-stranded DNA virus with a genome size of about 8000 base pairs.

[0003] More than 120 types of HPV have been identified, clinically divided into high-risk types and low-risk types according to their cancer-causing risks. Low-risk types are related to sexually transmitted warts or condyloma acuminatum, and generally do not induce cancer. Common low-risk types are 6, 11, 44, 81, etc.; high-risk types are necessary for cervical cancer and uterine intraepithelial neoplasia. Common high-risk types are 16, 18, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 6...

Claims

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