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Preparation method of efficient sensing electrode of lactic acid sensor

A sensing electrode and sensor technology, applied in the field of biosensors, can solve the problems of unsatisfactory detection, expensive detection reagents, cumbersome sample processing, etc., and achieve the effect of making up for low detection accuracy, high linear detection limit, and short response time

Active Publication Date: 2019-12-31
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, tetrazolium salt colorimetric method, p-hydroxybiphenyl colorimetric method, direct enzyme detection method, etc. are often used to detect lactic acid. These methods are cumbersome to process samples, expensive detection reagents, insufficient detection accuracy, and test results are greatly affected by many factors. The detection cannot meet the requirements of real-time and accuracy

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  • Preparation method of efficient sensing electrode of lactic acid sensor
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  • Preparation method of efficient sensing electrode of lactic acid sensor

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Embodiment 1

[0070] Embodiment 1 of the present invention provides a high-efficiency sensing electrode for a lactic acid sensor, the preparation method of which includes the preparation of LOX protease and the preparation process of electrode materials;

[0071] The preparation process of electrode materials includes the preparation process of graphene oxide and graphene quantum dot composite materials and the treatment process of glassy carbon electrodes;

[0072] The graphene oxide and graphene quantum dot composite material is prepared by dissolving graphene oxide and graphene quantum dots in ultrapure water at a concentration of 1.3 mg / mL, ultrasonicating for 2 hours; then reacting the resulting solution at 180°C for 14 hours; Then the reaction solution was centrifuged at 8000rpm for 6h; then it was dried in a vacuum oven at 60°C for 14h;

[0073] The particle size of graphene quantum dots is 5nm, the sheet diameter of graphene oxide is 250nm, and the weight ratio of graphene oxide to ...

Embodiment 2

[0088] Embodiment 2 of the present invention provides a high-efficiency sensing electrode for a lactic acid sensor, the preparation method of which includes the preparation of LOX protease and the preparation process of electrode materials;

[0089] The preparation process of electrode materials includes the preparation process of graphene oxide and graphene quantum dot composite materials and the treatment process of glassy carbon electrodes;

[0090] The graphene oxide and graphene quantum dot composite material is prepared by dissolving graphene oxide and graphene quantum dots in ultrapure water at a concentration of 2 mg / mL and ultrasonicating for 5 hours; then reacting the resulting solution at 200°C for 20 hours; then Centrifuge the resulting solution at 10000rpm for 9h; then dry it in a vacuum oven at 75°C for 20h;

[0091] The particle size of graphene quantum dots is 9nm, the sheet diameter of graphene oxide is 300nm, and the weight ratio of graphene oxide to graphene...

Embodiment 3

[0102] Embodiment 3 of the present invention provides a high-efficiency sensing electrode for a lactic acid sensor, the preparation method of which includes the preparation of LOX protease and the preparation process of electrode materials;

[0103] The preparation process of electrode materials includes the preparation process of graphene oxide and graphene quantum dot composite materials and the treatment process of glassy carbon electrodes;

[0104] The graphene oxide and graphene quantum dot composite material is prepared by dissolving graphene oxide and graphene quantum dots in ultrapure water at a concentration of 0.8 mg / mL and ultrasonicating for 1 h; then reacting the resulting solution at 150°C for 10 h; Then the reaction solution was centrifuged at 6000rpm for 3h; then it was dried in a vacuum oven at 45°C for 10h;

[0105] The particle size of graphene quantum dots is 3nm, the sheet diameter of graphene oxide is 150nm, and the weight ratio of graphene oxide to graph...

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Abstract

The invention, which relates to the technical field of biosensors, particularly provides an efficient sensing electrode of a lactic acid sensor and a preparation method thereof. The invention providesa preparation method of an efficient sensing electrode of a lactic acid sensor. The preparation method comprises steps of preparing LOX protease and preparing an electrode material. The step of preparing an electrode material includes a preparation process of a graphene oxide and graphene quantum dot composite material and a treatment process of a glassy carbon electrode.

Description

technical field [0001] The invention relates to the technical field of biosensors, and more specifically, the invention provides a high-efficiency sensing electrode of a lactate sensor and a preparation method thereof. Background technique [0002] Lactic acid is an important intermediate product in the metabolic process of the human body, which is produced by cells through anaerobic glycolysis when the human body exercises a lot. If it cannot be metabolized in time, it will lead to lactic acidosis, symptoms such as hypotension, confusion, lethargy, stupor and coma, and even permanent damage. Lactic acid can be used as a sensitive and reliable indicator to reflect tissue hypoxia, and it can evaluate the prognosis of patients, so it has certain practical value. [0003] At present, tetrazolium salt colorimetric method, p-hydroxybiphenyl colorimetric method, direct enzyme detection method, etc. are often used to detect lactic acid. These methods are cumbersome to process samp...

Claims

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Application Information

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IPC IPC(8): G01N27/30G01N27/327
CPCG01N27/308G01N27/3275
Inventor 周杰马婕妤庄妮莎薛瑞董维亮姜岷
Owner NANJING UNIV OF TECH
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