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Purpose of ATCA to preparation of medicines for tumors

An application and tumor technology, applied in the field of salts in the preparation of drugs for the treatment of tumors, can solve the problems of slowing down the translation and elongation rate of oncogenes, difficult to effectively inhibit the malignant phenotype of cancer cells, and limited use of puromycin.

Pending Publication Date: 2020-01-03
CHI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies in recent years have confirmed that cancer cells enhance their malignant phenotype by slowing down the translation elongation rate of oncogenes (Lian et al., Genome-wide and experimental resolution of relative translation elongation speed at individual gene level in human cells.PLoS Genetics(2016)12(2):e1005901), so it is difficult to effectively inhibit the malignant phenotype of cancer cells with a small dose
Due to this dilemma, cycloheximide has been withdrawn from the clinical practice of general cancer treatment, and the use of puromycin is also extremely limited

Method used

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  • Purpose of ATCA to preparation of medicines for tumors
  • Purpose of ATCA to preparation of medicines for tumors
  • Purpose of ATCA to preparation of medicines for tumors

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] In order to test whether the method of the present invention has an inhibitory effect on the malignant phenotype of cancer cells and whether it can kill normal cells, experiments on cell proliferation, cell migration and cytotoxicity are carried out. The experimental steps are as follows:

[0052] (1) Use cell culture medium at 37°C, 0.5% CO 2 Culture human lung cancer cell lines A549, H1299 and lung normal epithelial cells HBE in the environment;

[0053] (2) Add ATCA solution so that the final concentration reaches 0.5mg / mL, 1.0mg / mL, 1.5mg / mL, 2.0mg / mL respectively, shake well, and continue to culture cells in the original environment.

[0054] Detection of cell proliferation, cell migration, and cytotoxicity in steps (1) and (2).

[0055] The cell proliferation results of HBE, A549 and H1299 cells before and after administration are as follows: figure 1 shown. Depend on figure 1 It can be seen that with the increase of drug concentration, the proliferation rate...

Embodiment 2

[0059] In order to check whether the method of the present invention has inhibitory effect on cancer cells and whether normal cells are killed, the following experiments are carried out to suppress the malignant phenotype of cancer cells on the nude mouse xenograft tumor model:

[0060] (1) Take nude mice and inject lung cancer cell A549 subcutaneously;

[0061] (2) Wait for nude mice to form tumors, and discard nude mice without tumors;

[0062] (3) The mice in the treatment group use 0.2mL of ATCA solution (concentration 25mg / mL) for intragastric administration, so that the drug dose of ATCA in each mouse reaches 5 mg, and intragastric administration once a day; mice in the control group use an equal volume of normal saline ( NaCl) gavage;

[0063] (4) At the same time, the nude mice not injected with cancer cells were taken as the healthy group, and the same amount of ATCA and NaCl solution as the experimental group was administered orally.

[0064] The survival curves, t...

Embodiment 3

[0069] In order to test whether the method of the present invention has an inhibitory effect on the malignant phenotype of lung cancer and other types of cancer cells, and whether it can kill normal cells, cell apoptosis and cell proliferation detection experiments are carried out. The experimental steps are as follows:

[0070] (1) Use cell culture medium at 37°C, 0.5% CO 2 Human lung cancer cell lines A549, H1299 and lung normal epithelial cells HBE, ovarian cancer cell line ES-2, liver cancer cell lines HCCLM3, HCCLM6 and MHCC97H were cultured in the environment;

[0071](2) Add ATCA solution, set the dosing concentration gradient, so that the final concentration reaches 0.125mg / mL, 0.25mg / mL, 0.5mg / mL, 0.75mg / mL, 1.0mg / mL, shake well, and continue in the original environment Cultured cells.

[0072] Use Annexin V and propidium iodide to stain human lung cancer cells A549, H1299 and normal lung epithelial cells for HBE, and then use flow cytometry to detect the results. ...

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Abstract

The invention discloses a purpose of ATCA (Aurintricarboxylic acid, aurin tricarboxylic acid) to preparation of medicines for treating tumors. Through experiment, the inventor of the invention finds that through specifically intervening a translation initiation process, the ATCA can enable cancer cells not to strengthen malignant phenotypes through slowing down cancer gene translation elogation rate, so that the malignant phenotypes of the cancer cells can be restrained, besides, lethal effects on normal cells can be minimized, the effect of resisting cancer is achieved, and besides, the sideeffects are effectively controlled. The ATCA disclosed by the invention has important application value on tumor treatment.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to the use of ATCA (Aurintricarboxylic acid, aurintricarboxylic acid, also known as ATA) and a pharmaceutically acceptable salt thereof in the preparation of drugs for treating tumors. Background technique [0002] In recent years, the incidence of cancer has increased year by year, causing widespread harm, causing great suffering to patients and heavy economic burden on society. Usually anti-cancer methods include surgery, chemotherapy, radiotherapy, immunotherapy, etc., among which radiotherapy and immunotherapy need to target specific cancer types and conditions, and are not widely applicable; Cancer that has metastasized is not effective. Therefore, chemotherapy is the only treatment that works for almost all cancers. Chemotherapy can be divided into two categories: common chemotherapy and targeted therapy. Common chemotherapy is usually based on the mechanism of drug-indu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/194A61P35/00
CPCA61K31/194A61P35/00
Inventor 陈洋张弓余卓
Owner CHI BIOTECH CO LTD
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