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Coumarin fluorescence array sensor, and preparation method and application thereof

An array sensor, coumarin technology, applied in chemical instruments and methods, fluorescence/phosphorescence, instruments, etc., can solve the problems of high cost of sensor array preparation, easy aggregation of gold nanoparticles, poor stability of sensing system, etc., to achieve Inexpensive, easy to build, fast effects

Active Publication Date: 2020-01-10
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The material used is gold nanoparticles + cysteine / glutathione / melamine, which can identify six metal ions, Ti4+, Cr3+, Mn2+, Fe3+, Pb2+ and Sn4+. This detection method requires the use of gold nanoparticles, and As a noble metal, gold is expensive, and the preparation cost of the sensor array is high, and the stability of the sensing system is poor, and the gold nanoparticles themselves are prone to aggregation, resulting in sensor failure, and the sensor can only be used for Ti 4+ 、Cr 3+ , Mn 2+ , Fe 3+ , Pb 2+ and Sn 4+ The identification of these six metal ions has limited recognition ability

Method used

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  • Coumarin fluorescence array sensor, and preparation method and application thereof
  • Coumarin fluorescence array sensor, and preparation method and application thereof
  • Coumarin fluorescence array sensor, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Embodiment 1 Construction of array sensor R1SA

[0041] 1.1 Preparation of stock solution A: Accurately weigh R1 (5.0 mg) and dissolve it in 5.0 mL of ethanol to obtain R1 stock solution A with a concentration of 3 mM;

[0042] 1.2 Add 30 μL of the above stock solution A to 3 mL of methanol, dimethyl sulfoxide, ethanol and acetonitrile in sequence to obtain R1 in methanol, dimethyl sulfoxide, ethanol and acetonitrile solutions. The concentration of R1 in the solution is all 10 μM. Each solution is four sensing units of the array sensor of the present invention;

[0043] 1.3 The methanol, dimethyl sulfoxide, ethanol and acetonitrile solutions of the above four R1s form an array sensor R1SA for the detection of metal ions.

Embodiment 2

[0044] Embodiment 2 establishes array sensor training matrix

[0045] 2.1. Preparation of stock solution B: Dilute the R1 stock solution A in Step 1.1 of Example 1 15 times with ethanol to obtain a stock solution B with an R1 concentration of 0.2 mM;

[0046] 2.2. Prepare a known metal ion stock solution with a concentration of 6mM: mix 25 kinds of metal ions Cs + , Fe 3+ , Ca 2+ 、Cu 2+ , Pb 2+ , Mg 2+ 、K + , Mn 2+ , Hg 2+ 、Ni 2+ 、Cr 3+ 、Al 3+ , Zn 2+ 、Cd 2+ , Sn 2+ 、Na + , V 3+ , Li + 、Zr 4+ 、Ag + 、Ba 2+ , Fe 2+ 、Mo 6 + 、 Bi 3+ 、Co 2+ The corresponding inorganic salts were respectively dissolved in pure water to obtain a known metal ion stock solution with a concentration of 6 mM for use;

[0047] 2.3. Divide every 5 rows×4 columns of the 96-well plate into a group, and add 180 μL of methanol, dimethyl sulfoxide, ethanol and acetonitrile to the 1st to 4th columns of each group; Pipette 10 μL of 0.2 mM stock solution B from the well; then add 10 μL of...

Embodiment 3

[0055] Example 3 Identification of 0.5 μM metal ions by the array sensor R1SA

[0056] 3.1. Prepare 0.1mM metal ion stock solution: Cs + , Fe 3+ , Ca 2+ 、Cu 2+ , Pb 2+ , Mg 2+ 、K + , Mn 2+ , Hg 2+ 、Ni 2 + 、Cr 3+ 、Al 3+ , Zn 2+ 、Cd 2+ , Sn 2+ 、Na + , V 3+, Li + 、Zr 4+ 、Ag + 、Ba 2+ , Fe 2+ 、Mo 6+ 、 Bi 3+ 、Co 2+ :

[0057] 3.2. Pipette 190 μL of Methanol, DMSO, Ethanol, and MeCN into each column of each 5-row×4-column area of ​​the 96-well plate;

[0058] 3.3. Pipette 10 μL of 0.2 mM R1 dilution (stock solution B prepared in Example 2) into each well in the area of ​​step 3.2;

[0059] 3.4. Pipette 1 μL of 0.1 mM metal ion stock solution and add to step 3.3 to obtain a test solution with a metal ion concentration of 0.5 μM. Measure the fluorescence intensity of each well of the 96-well plate with a microplate reader, and use origin to perform linear discriminant analysis or principal component analysis, which can effectively identify 25 kinds of metal i...

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Abstract

The invention discloses a coumarin fluorescence array sensor, and a preparation method and an application thereof. The preparation method of the coumarin fluorescence array sensor (R1SA) comprises thesteps of: respectively dissolving a coumarin compound R1 in methanol, dimethylsulfoxide, ethanol or acetonitrile or other organic solvents to construct and obtain a plurality of corresponding sensingunits, wherein the plurality of corresponding sensing units constitute the coumarin fluorescence array sensor. The fluorescence array sensor R1SA constructed by the preparation method disclosed by the invention can be used for the quantitative, accurate and rapid detection of 25 common metal ions in water samples and serum environments, only a specific solution needs to be formulated in the detection process according to the steps, and a final result can be obtained by reading data on a microplate reader, thereby being convenient and fast. The reading of the microplate reader only requires about 1 minute, so that the speed is high, and the efficiency is high.

Description

technical field [0001] The invention belongs to the field of detection of metal ions in environmental water samples and biological fluids, and in particular relates to a coumarin fluorescent array sensor and its preparation method and application. Background technique [0002] In daily life, some metal ions are necessary for human life activities, such as Na + , K + , Mg 2+ , Ca 2+ , Fe 2+ , Zn 2+ , Cu 2+ , Mn 2+ etc., their absence and concentration changes all affect the normal operation of body functions; on the other hand, with the increasingly serious environmental problems, some heavy metal ions such as Cd 2+ , Hg 2+ , Pb 2+ It is easier to be ingested by the human body through drinking water and other ways, thus endangering human health. It is of great practical significance to develop methods for detecting the types and concentrations of metal ions in environmental water samples and biological fluids to provide powerful data for understanding the pollution ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N33/533G01N33/543C09K11/06C09K11/02
CPCC09K11/02C09K11/06C09K2211/1029C09K2211/1088G01N21/6428G01N21/643G01N33/533G01N33/543
Inventor 丁煜宾曹芝瑗张雪斌丁宁徐进章维华
Owner NANJING AGRICULTURAL UNIVERSITY
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