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Novel electrochemical method of detecting copper ions, phosphate ions and alkaline phosphatase and application thereof

A technology of phosphate ion and copper ion, which is applied in the field of functional biomaterials and biosensing to achieve accurate results, less time-consuming, and high-sensitivity detection

Active Publication Date: 2020-01-10
GUIZHOU LIPING AOJIE CARBON
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no reports on the detection of Cu(II), PPi, ALP and their inhibitors using a new electrochemical method based on copper-based nucleic acid-like coordination polymers at home and abroad.

Method used

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  • Novel electrochemical method of detecting copper ions, phosphate ions and alkaline phosphatase and application thereof
  • Novel electrochemical method of detecting copper ions, phosphate ions and alkaline phosphatase and application thereof
  • Novel electrochemical method of detecting copper ions, phosphate ions and alkaline phosphatase and application thereof

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Embodiment 1

[0035] The preparation of embodiment 1 sensor

[0036] The technical solution adopted by the present invention to solve the above technical problems is: a novel electrochemical method and application for detecting copper ions, phosphate ions and alkaline phosphatase, the specific steps are as follows:

[0037] Preparation of Electrode 1:

[0038] First, the glassy carbon electrode (GCE, 3 mm in diameter) was polished on the suede with Al2O3 powder (0.05 μm) for 5 min. N 2 Blow dry to get a bare glassy carbon electrode. The electrode is recorded as Electrode 1;

[0039] Preparation of Electrode 2:

[0040] Graphene was dispersed in HAc-NaAc (0.2M, pH 5.0) buffer solution, the control concentration was 1.0mg·mL -1 , Ultrasonic dispersion 2h. Place the bare GCE electrode in the above solution, and use cyclic voltammetry at a potential range of -1.5V to +0.5V at 10mV s -1 The sweep speed scans 10 laps. Get the GO modified electrode, which is denoted as Electrode 2;

[004...

Embodiment 2

[0045] The detection of embodiment 2 Cu(II)

[0046] In the preparation process of Example 1 Electrode 3, the concentration of Cu(II) was changed, and the final concentration of Cu(II) was controlled to be: 0, 0.01, 0.02, 0.05, 0.1, 0.2, 0.5, 0.8, 1, 2, 5, 10, 20, 40, 80, 150, 250, 500 μM, a series of electrochemical biosensors were prepared as in Example 1. Experimental results such as figure 2 As shown, within a certain range, the greater the target concentration, the more obvious the current response. The linear correlation equation of the current response of the sensor to the logarithmic value of the Cu(II) concentration is I=-40.26lg Cu(II) -106.40, R 2 =0.9994, the linear range is 0.01-250 μM, and the detection limit is 0.0021 μM, indicating that the sensor can achieve highly sensitive detection of Cu(II) concentration.

Embodiment 3

[0047] The detection of embodiment 3 PPi

[0048] During the preparation process of Example 1 Electrode 3, the concentration of PPi was changed to control the final concentration of PPi to be respectively: 0, 0.01, 0.02, 0.05, 0.1, 0.25, 0.5, 0.8, 1, 2, 5, 10, 20, 50, 100, 200, 500, 800 μM, a series of electrochemical biosensors were prepared as in Example 1. Experimental results such as image 3 As shown, within a certain range, the greater the target concentration, the lower the current response, which proves that PPi forms a complex with Cu(II). The current response of the sensor to the linear correlation equation of the logarithmic value of the PPi concentration is I=37.37lg PPi -112.09, R 2 =0.9981, the linear range is 0.01-200 μM, and the linear correlation equation shows that the detection limit is 0.0035 μM. It shows that the sensor can realize highly sensitive detection of PPi concentration.

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Abstract

The invention discloses a novel electrochemical method of detecting copper ions, phosphate ions and alkaline phosphatase and application. CoA interacts with Cu(II) to form a CoA-Cu(II) coordination polymer, which is named CoA-Cu(II)CP, and can catalyze decomposition of H<2>O<2>. In the presence of PPi, PPi can form a Cu(II)-PPi complex with Cu(II) to prevent CoA-Cu(II)CP from being formed. Decomposition of PPi and releasing of Cu(II) are caused through enzymatic decomposition reaction of ALP, CoA-Cu(II)CP is formed, and catalytic decomposition reaction of H<2>O<2> occurs. On the basis thereof,electrochemical sensors of the copper ions, the phosphate ions and the alkaline phosphatase are constructed, and compared with devices of traditional detection methods, the electrochemical biosensorsconstructed on the basis of the nucleic acid-like coordination polymer of copper have the characteristics of simple preparation, high efficiency, high sensitivity and the like.

Description

technical field [0001] The invention relates to an electrochemical biosensor, in particular to a novel electrochemical method and application for detecting copper ions, phosphate ions and alkaline phosphatase, and belongs to the technical field of functional biomaterials and biosensing. Background technique [0002] Understanding highly selective and sensitive molecules or ions in biology, such as copper ions (Cu(II)), phosphate ions (PPi) and alkaline phosphatase (ALP), are essential for studying the physiological and The pathological process is critical. Cu(II) is an essential trace element for humans and animals, and excess Cu(II) can cause oxidative stress and lead to neurodegenerative diseases such as Alzheimer's disease, Parkinson's disease, and Wilson's disease. PPi is an important metabolite in various biochemical processes. Also, many studies have shown that PPi can be used as an indicator of certain diseases, such as arthritis and cartilage calcification. ALP, w...

Claims

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Application Information

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IPC IPC(8): G01N27/333
CPCG01N27/333
Inventor 胡宇芳胡丹丹詹甜玉郑宇迪刘鑫达冉平建王邃郭智勇
Owner GUIZHOU LIPING AOJIE CARBON
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