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Preparation method of ultra-small-size iron oxide nanoparticles and application thereof

A technology of iron oxide nanometers and iron oxide particles, which is applied in the field of cell tracers, can solve the problems of increasing particle size and weakening the positive enhancement performance of ultra-small iron oxide, and achieves simple preparation methods, solving the problem of strong magnetic susceptibility, The effect of improving intake efficiency

Inactive Publication Date: 2020-01-14
SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

During the transfer of oil-soluble ultra-small iron oxide particles to the water phase, on the one hand, it is easy to agglomerate and cause the particle size to become larger; on the other hand, organic molecules adsorbed on the surface of iron oxide reduce the chance of contact between the iron oxide core and water molecules, greatly weaken Positive enhancing properties of ultra-small iron oxides

Method used

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  • Preparation method of ultra-small-size iron oxide nanoparticles and application thereof
  • Preparation method of ultra-small-size iron oxide nanoparticles and application thereof
  • Preparation method of ultra-small-size iron oxide nanoparticles and application thereof

Examples

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Effect test

Embodiment 1

[0038] Preparation of Ultra-Small Size Iron Oxide Particles

[0039] S1. Mix 1mmol of iron acetylacetonate and 25ml of diethylene glycol and add them into a three-neck round bottom flask, wherein the concentration of iron acetylacetonate in diethylene glycol is 0.04mol / L, and feed argon for 15min; then Heat at a rate of 3°C / min to 120°C, keep warm for 1 hour to remove water; then heat at a rate of 3°C / min to 200°C, the reaction system gradually turns from reddish brown to a black solution, heat to reflux for 0.5h, and cool naturally to room temperature. Prepare ultra-small iron oxide mixed liquid;

[0040] S2. Add ethyl acetate to the super-small iron oxide mixture prepared in step S1 after cooling to precipitate the iron oxide nanoparticles, then centrifuge at 8000rpm for 20min, remove the supernatant; then disperse the precipitate in ethanol , and then precipitated with ethyl acetate and centrifuged three times to obtain ultra-small iron oxide nanoparticles;

[0041] S3. T...

Embodiment 2

[0043] Preparation of Ultra-Small Size Iron Oxide Particles

[0044] S1. Mix 20mmol of iron acetylacetonate and 1000ml of diethylene glycol and add them into a three-neck round bottom flask, wherein the concentration of iron acetylacetonate in diethylene glycol is 0.02mol / L, and feed argon for 25min; then Heating at a rate of 4 °C / min to 130 °C, keeping it warm for 1.5 hours to remove water; then heating at a rate of 7 °C / min to 210 °C, the reaction system gradually turns from reddish brown to a black solution, heated to reflux for 1 hour, and cooled naturally to room temperature. Prepare ultra-small iron oxide mixed liquid;

[0045] S2. Add ethyl acetate to the super-small iron oxide mixture prepared in step S1 after cooling to precipitate the iron oxide nanoparticles, then centrifuge at 8000rpm for 20min, remove the supernatant; then disperse the precipitate in ethanol , and then precipitated with ethyl acetate and centrifuged three times to obtain ultra-small iron oxide na...

Embodiment 3

[0048] Preparation of Ultra-Small Size Iron Oxide Particles

[0049] S1. Mix 32mmol of iron acetylacetonate and 200ml of diethylene glycol and add them into a three-neck round bottom flask, wherein the concentration of iron acetylacetonate in diethylene glycol is 0.16mol / L, and feed argon for 25min; then Heating at a rate of 5 °C / min to 100 °C, keeping it warm for 2 hours to remove water; then heating at a rate of 10 °C / min to 220 °C, the reaction system gradually changed from reddish brown to a black solution, heated to reflux for 2 hours, and naturally cooled to room temperature to prepare Obtain ultra-small size iron oxide mixture;

[0050] S2. Add ethyl acetate to the super-small iron oxide mixture prepared in step S1 after cooling to precipitate the iron oxide nanoparticles, then centrifuge at 8000rpm for 20min, remove the supernatant; then disperse the precipitate in ethanol , and then precipitated with ethyl acetate and centrifuged three times to obtain ultra-small iro...

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Abstract

The invention provides a preparation method of ultra-small-size iron oxide nanoparticles. The method comprises the following steps: dissolving ferric acetylacetonate in diethylene glycol with the concentration of 0.02-0.16 mol / L in the diethylene glycol, carrying out thermal decomposition on ferric acetylacetonate in an inert gas environment at 200-220 DEG C until a reaction system is converted into a black solution from reddish brown, and carrying out steps of heating reflux, cooling, separating and purifying. The invention further provides an application of the ultra-small-size iron oxide nanoparticles prepared by the preparation method in preparation of an MRI cell tracer. The preparation method is simple, the preparation method is simple, various parameters are easy to control, the prepared iron oxide nanoparticles are accurately controlled within the range of 2-5 nm, the prepared iron oxide nanoparticles are very sensitive to the influence of water quantum MRI signals of marked cells, the positive contrast of the ultra-small-size iron oxide nanoparticles serving as a contrast agent is enhanced, and positive enhancement is displayed for magnetically marked cells in an MRI image.

Description

technical field [0001] The invention relates to the technical field of cell tracers, in particular to a preparation method and application of ultra-small iron oxide nanoparticles. Background technique [0002] The combination of magnetic resonance imaging technology and cell magnetic labeling technology provides a non-damaging tracer method for studying biological behaviors such as cell distribution, migration and homing in vivo. Using MRI images to track cells in vivo usually requires MR contrast agents to magnetically label cells. Currently, negative, larger particle size iron oxide contrast agents are used in most studies. For example, commercial dextran-coated superparamagnetic iron oxide particles (superparamagnetic iron oxide, SPIO, usually with a hydrated particle size greater than 50 nm) and micron-sized particles of iron oxide (micron-sized particles of iron oxide, MPIO). These two types of iron oxide contrast agents have a strong magnetic susceptibility effect, w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/18B82Y25/00B82Y40/00C01G49/06
CPCA61K49/1836B82Y25/00B82Y40/00C01G49/06C01P2004/64
Inventor 易佩伟王慧杨晓冬
Owner SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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