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Preparation method for low THC organic semen cannabis protein powder

A technology of hemp protein and protein powder, which is applied in the field of plant protein extraction, can solve the problems of hidden danger of THC poisoning, complicated preparation process, low product purity, etc., and achieves the effects of low production cost, simple process and improved extraction rate.

Active Publication Date: 2020-01-14
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the existing problem of extracting protein powder from hemp seeds, the preparation process is complicated, the product purity is low, and the contained THC is prone to poisoning hidden dangers, it provides A Low THC Organic Hemp Protein Powder

Method used

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Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0022] Step 1: removing impurities and foreign matters in the hemp seeds, mechanically crushing the hemp seeds with a rail-type crusher, and obtaining hemp seeds with a particle size of 10 mesh;

[0023] Step 2: Carry out low-temperature cold pressing on the hemp seed granules, separate the oil residue, crush and sieve the cold-pressed residue to obtain cold-pressed residue particles with a particle size of 100 mesh, and use protease Alcalase 2.4L to enzymatically hydrolyze the cold-pressed residue particles , the enzymolysis conditions are: the mass ratio of cold-pressed slag to water is 1:8, the enzymolysis temperature is 45°C, the enzymolysis pH is 8.0, and the enzymolysis time is 60 minutes; after the enzymolysis is completed, the enzyme is inactivated, and the enzyme inactivation temperature is 80°C. The enzyme inactivation time is 1 min. After the enzyme inactivation is completed, the mixture is subjected to three-phase separation to obtain hemp oil, a solution containing...

specific Embodiment approach 2

[0026] Step 1: removing impurities and foreign matter in the hemp seeds, mechanically crushing the hemp seeds with a rail-type crusher, and obtaining hemp seeds with a particle size of 15 mesh;

[0027] Step 2: Carry out low-temperature cold pressing on the hemp seed particles, separate the oil residue, crush and sieve the cold-pressed residue to obtain cold-pressed residue particles with a particle size of 110 mesh, and use protease Alcalase 2.4L to enzymatically hydrolyze the cold-pressed residue particles , the enzymolysis conditions are: the mass ratio of cold-pressed slag to water is 1:9, the enzymolysis temperature is 50°C, the enzymolysis pH is 9.0, and the enzymolysis time is 70min; The enzyme inactivation time is 1.5min. After the inactivation is completed, the mixture is subjected to three-phase separation to obtain the hemp oil, the solution containing THC and hemp protein, and the residue.

[0028] Step 3: Carry out cavitation jet treatment to the solution containi...

specific Embodiment approach 3

[0030] Step 1: removing impurities and foreign matter in the hemp seeds, mechanically crushing the hemp seeds with a rail-type crusher, and obtaining hemp seeds with a particle size of 20 mesh;

[0031] Step 2: Carry out low-temperature cold pressing on the hemp seed particles, separate the oil residue, crush and sieve the cold-pressed residue to obtain cold-pressed residue particles with a particle size of 120 mesh, and use protease Alcalase 2.4L to enzymatically hydrolyze the cold-pressed residue particles , the enzymolysis conditions are: the mass ratio of cold-pressed slag to water is 1:10, the enzymolysis temperature is 65°C, the enzymolysis pH is 10.0, and the enzymolysis time is 80min; The enzyme inactivation time is 2 minutes. After the enzyme inactivation is completed, the mixture is subjected to three-phase separation to obtain hemp oil, a solution containing THC and hemp protein, and a residue.

[0032] Step 3: Carry out cavitation jet treatment to the solution cont...

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Abstract

The invention discloses a preparation method for low THC organic semen cannabis protein powder, belongs to the field of plant protein extraction and aims at solving the problems of complex preparationprocess, low purity of a product and easiness in poisoning hazards with containing THC of existing protein powder extraction from semen cannabis. The preparation method comprises the following stepsof removing impurities and foreign matters in the semen cannabis, and performing mechanical crushing by adopting a grinding rail type crusher; and performing low-temperature cold pressing, crushing, sieving, enzymolysis, enzyme deactivation, three-phase separation, cavitation jet treatment, pressure direct injection sterilization, film falling concentration and centrifugal spray drying. In the preparation process disclosed by the invention, THC is destroyed by pressure direct injection sterilization to an extremely large extent is treated by applying jet cavitation treatment under the effectsof high pressure, shearing and cavitation, and the obtained THC as a product is not detected. The preparation method is simple in process, high in automation degree and low in production cost and is capable of greatly increasing the extraction rate of the semen cannabis protein; and the obtain semen cannabis protein is low in denaturation degree and has good gel property and emulsifying property.

Description

technical field [0001] The invention relates to a preparation method of low-THC organic hemp protein powder, which belongs to the field of vegetable protein extraction. Background technique [0002] The effect of diuresis, dredging stranguria and blood circulation is a moisturizing medicine that is both medicine and food. Modern research shows that hemp seed has good effects of lowering blood pressure, labor pain, anti-inflammation, and anti-thrombosis. Long-term consumption has a good auxiliary effect on chronic neuritis, paralysis, ulcers, and gynecological diseases. Hemp seed cake is the residue after extracting hemp seed oil from hemp seeds, the protein content of which is as high as 56%, and contains various essential amino acids needed by the human body, especially rich in arginine, lysine and glutamine acid, is a very good vegetable protein [0003] Hemp seeds contain phenolic substances that are easily oxidized, which will remain in the processed hemp seed cakes. A...

Claims

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Application Information

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IPC IPC(8): C07K1/00C12P21/06
CPCC07K1/00C12P21/06
Inventor 齐宝坤江连洲朱建宇黄莉曹亮
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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