Preparation method of squid ink bioactive peptide

A technology of squid ink and active peptide, which is applied in the field of biological extraction, can solve the problems of squid leftovers polluting the environment, etc., and achieve the effects of complete protein decomposition, convenient processing, and high enzymatic hydrolysis efficiency

Pending Publication Date: 2020-01-14
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to overcome the problem that squid scraps are discarded and pollute the environment, the present invention provides a method for preparing squid ink active peptides, which effectively utilizes one of the squid scraps to prepare active peptides with rich physiological functions, turning waste into treasure

Method used

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  • Preparation method of squid ink bioactive peptide
  • Preparation method of squid ink bioactive peptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] (a) Squid ink collected in the above-mentioned squid processing device;

[0027](b) Mix squid ink and water at a volume ratio of 1:20, adjust the pH value to 1, use 0.1% pepsin to enzymatically hydrolyze at 30 °C for 3 h, then adjust the pH value to 7, and use 0.1% compound protease to enzymatically digest at 50 °C Decompose 1 h, the mass ratio of composite protease is alkaline protease: papain: flavor protease=1:2:4, makes enzymolysis liquid, centrifuges 15 min under 6000 rpm and gets supernatant;

[0028] (c) Add 2 times the volume of 5% (m / v) trichloroacetic acid solution to the supernatant of the enzymatic hydrolysis solution, mix well and let stand at room temperature for 60 min, centrifuge to get the supernatant, dialyze and desalt, Freeze-drying to obtain squid ink active peptide.

Embodiment 2

[0030] (a) Squid ink collected in the above-mentioned squid processing device;

[0031] (b) Mix squid ink and water at a volume ratio of 1:15, adjust the pH value to 4, use 0.1% pepsin to enzymatically hydrolyze at 40 °C for 1 h, then adjust the pH value to 9, and use 0.1% compound protease to enzymatically digest at 60 °C Decompose 1 h, the mass ratio of composite protease is alkaline protease: papain: flavor protease=3:4:2, makes enzymolysis liquid, centrifuges 5 min under 8000 rpm to get supernatant;

[0032] (c) Add an equal volume of 20% (m / v) trichloroacetic acid solution to the supernatant of the enzymatic hydrolysis solution, mix well and let stand at room temperature for 60 min, centrifuge to get the supernatant, dialyze and desalt, freeze Dried to obtain squid ink active peptide.

Embodiment 3

[0034] (a) Squid ink collected in the above-mentioned squid processing device;

[0035] (b) Mix squid ink and water at a volume ratio of 1:10, adjust the pH value to 3, use 0.1% pepsin to enzymatically hydrolyze at 35 °C for 2 h, then adjust the pH value to 8, and use 0.1% compound protease to enzymatically digest at 55 °C Decompose 2 h, the mass ratio of compound protease is alkaline protease: papain: flavor protease=1:2:2, makes enzymolysis liquid, centrifuges 5 min under 8000 rpm to get supernatant;

[0036] (c) Add an equal volume of 15% (m / v) trichloroacetic acid solution to the supernatant of the enzymatic hydrolysis solution, mix well and let stand at room temperature for 60 min, centrifuge to get the supernatant, dialyze and desalt, freeze Dried to obtain squid ink active peptide.

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Abstract

The invention relates to the technical field of biology extraction. In order to solve the problem that squid scraps are discarded to pollute environment, the invention provides a preparation method ofsquid ink bioactive peptide. The preparation method comprises the following steps of (a) placing squids in a squid treatment device, taking ink sacs of the squids and collecting squid ink; (b) mixingthe squid ink with water in the volume ratio being 1 to (10-20), regulating pH value to 1-4, performing enzymolysis at 30-40 DEG C with 0.1% pepsin for 1-3h, then regulating the pH value to 7-9, performing enzymolysis at 50-60 DEG C with a 0.1% compound protein enzyme for 1-2h so as to prepare enzymatic hydrolysate, performing centrifugation, and taking supernatant; and (c) adding a 5-20%(m/v) trichloroacetic acid solution to the enzymatic hydrolysate supernatant, performing uniform mixing, performing standing, performing centrifugation, taking supernatant, performing desalination and then performing freeze-drying to obtain the squid ink bioactive peptide. The ink among the squid scraps is effectively used, so that the bioactive peptide being rich in physiological functions is prepared, and wastes are turned into wealth.

Description

technical field [0001] The invention relates to the technical field of biological extraction, in particular to a preparation method of squid ink active peptide. Background technique [0002] Squid is a common name for squid and squid among cephalopod marine animals. Squid has gradually become an important protein resource for humans because of its delicious taste and rich nutrition, but the inedible parts of squid are often not effectively utilized. These wastes include ink sacs, viscera, and skin, which account for about 30% of squid mass. Ink sacs account for 11.3% of squid mass. The general treatment method for these wastes is to bury them on the spot. This is not only a waste of fishery resources, but also aggravates environmental pollution. [0003] There is ink in the ink sac of squid. The biomacromolecule polymer contained in squid ink is the name of the biomacromolecule polymer. It is the only natural endogenous biopolymer known to protect organisms from radiation d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06
CPCC12P21/06
Inventor 刘宇姜维胡世伟李世杰
Owner ZHEJIANG OCEAN UNIV
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