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A kind of mesoporous silica drug-loaded nanoparticles and its preparation method and application

A technology of mesoporous silica and drug-loaded nanometers is applied in the field of medicine to achieve obvious tumor cell targeting ability, feasible and reliable operation, and good photothermal treatment effect.

Active Publication Date: 2022-03-25
XIAN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Therefore, using PDA as a reaction platform to construct a mesoporous silica drug-loading system (MSNs@PDA-PEOz-iRGD) co-modified by the tumor-directed penetration peptide iRGD and the pH-responsive material PEOz, is expected to achieve tumor penetration, triggered release and photothermal The purpose of treatment, the current MSNs@PDA-PEOz-iRGD system and its application in the field of tumor treatment have not been reported

Method used

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  • A kind of mesoporous silica drug-loaded nanoparticles and its preparation method and application
  • A kind of mesoporous silica drug-loaded nanoparticles and its preparation method and application
  • A kind of mesoporous silica drug-loaded nanoparticles and its preparation method and application

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Embodiment 1

[0051] A preparation method of mesoporous silica drug-carrying nanoparticles, which is specifically implemented according to the following steps:

[0052] Step 1, Synthesis of MSN

[0053] 250 mg of CTAB was weighed and dissolved in 120 mL of deionized aqueous solution, then 875 μL of 2M NaOH was added, and vigorously stirred at 60° C. for 30 min. After CTAB was completely dissolved, 1.25 mL of TEOS was slowly added, and stirring was continued for 2 h. After the reaction, the product was left to cool to room temperature, centrifuged at 10,000 rpm for 5 min, washed three times with deionized water and ethanol, and finally extracted and refluxed with ammonium nitrate-containing ethanol solution (4 g / L) for 24 h to remove the template agent. After obtaining the sample, It was washed three times with deionized water and absolute ethanol, and dried in a vacuum drying oven at 60 °C for 12 h, marked as MSN.

[0054] Step 2, MSN-NH 2 Synthesis

[0055] Weigh 200mg MSN powder into ...

Embodiment 2

[0063] A preparation method of mesoporous silica drug-carrying nanoparticles, which is specifically implemented according to the following steps:

[0064] Step 1, Synthesis of MSN

[0065]Weigh 300 mg of CTAB and dissolve it in 150 mL of deionized aqueous solution, then add 1000 μL of 2M NaOH, and stir vigorously at 70 °C for 40 min. After CTAB was completely dissolved, 1.5 mL of TEOS was slowly added, and stirring was continued for 3 h. After the reaction, the product was left to cool to room temperature, centrifuged at 12,000 rpm for 8 min, and washed 4 times with deionized water and ethanol, respectively. Finally, an ethanol solution containing ammonium nitrate (5g / L) was used to extract and reflux for 36h to remove the template agent. After obtaining the sample, it was washed with deionized water and anhydrous ethanol for 4 times, dried in a vacuum drying oven at 70 °C for 12h, and marked as MSN. .

[0066] Step 2, MSN-NH 2 Synthesis

[0067] Weigh 300 mg of MSN powde...

Embodiment 3

[0075] A preparation method of mesoporous silica drug-carrying nanoparticles, which is specifically implemented according to the following steps:

[0076] Step 1, Synthesis of MSN

[0077] 400 mg of CTAB was weighed and dissolved in 200 mL of deionized aqueous solution, then 1200 μL of 2M NaOH was added, and vigorously stirred at 80 °C for 45 min. After CTAB was completely dissolved, 2.0 mL of TEOS was slowly added, and stirring was continued for 3 h. After the reaction, the product was allowed to stand to cool to room temperature, centrifuged at 15,000 rpm for 10 min, and washed with deionized water and ethanol for 5 times, respectively. Finally, the template agent was removed by extraction and reflux with ethanol solution containing ammonium nitrate (6g / L) for 48h. After the sample was obtained, it was washed with deionized water and absolute ethanol for 5 times, and dried in a vacuum drying box at 80 °C for 24h, marked as MSN. .

[0078] Step 2, MSN-NH 2 Synthesis

[0...

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Abstract

The invention discloses a mesoporous silica drug-loaded nanoparticle, which uses mesoporous silica as a drug-carrying carrier, uses mesoporous silica as a drug-carrying carrier, wraps a polydopamine layer on its outside, and passes through a Schiff base The addition reaction connects the tumor-guiding penetration peptide iRGD and poly(2-ethyl-2-oxazoline) to obtain the mesoporous silica drug-loaded nanoparticles MSN@PDA-PEOz-iRGD of the present invention, and the present invention also discloses In the preparation method of the above-mentioned drug-loaded nanoparticles, the prepared drug-loaded nanoparticles are loaded on the anticancer drug, and the drug has obvious pH-responsive release, has obvious tumor cell targeting ability, and has good photothermal therapy effect , good anti-tumor effect in vivo and in vitro, providing a theoretical basis for the research of new targeted drug delivery systems.

Description

technical field [0001] The invention belongs to the technical field of medicine, in particular to a drug-loaded nanoparticle of mesoporous silica, and also relates to a preparation method and application of the above-mentioned drug-loaded nanoparticle. Background technique [0002] Malignant tumors seriously threaten human health and life safety. Chemotherapy (chemotherapy) is one of the important means of clinical treatment of cancer. Antitumor drug nano-delivery systems have become a hot spot in the current research on chemotherapeutic drug preparations due to their small size, which can significantly improve the solubility of chemotherapeutic drugs, improve drug stability, and prolong their blood circulation time. Among them, mesoporous silica has been shown to be one of the most promising nanocarriers for drug delivery. The pore size of mesoporous silica is between 2 and 50 nm. It has the advantages of large specific surface area, high drug loading, good biocompatibili...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/51A61K47/04A61K47/42A61K47/34A61K31/337A61K31/704A61K41/00A61P35/00
CPCA61K9/5115A61K9/5169A61K9/5146A61K9/5192A61K31/337A61K31/704A61K41/0052A61P35/00A61K2300/00
Inventor 王小宁梁晓燕闫梦茹马远涛高迎春赵宁
Owner XIAN MEDICAL UNIV
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