Method for enhancing luminous intensity of squamous-cell carcinoma antigen during electrochemical luminescence detection

A squamous cell carcinoma and luminescent detection technology, which is applied in the direction of chemiluminescence/bioluminescence, analysis by making materials undergo chemical reactions, and measurement devices, can solve the problems of unfavorable rapid analysis, tedious, high-tech requirements, etc., and achieve improvement Electrochemiluminescent intensity, accelerated electron transfer rate, and good selectivity

Active Publication Date: 2020-01-17
GUANGXI NORMAL UNIV
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Problems solved by technology

Among the existing analysis methods, some methods require expensive experimental equipment and high technical requirements, while others are more cumbersome, which is not conducive to rapid analysis
However, there is no Fe-MIL-88-NH 2 Related reports on the construc...

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  • Method for enhancing luminous intensity of squamous-cell carcinoma antigen during electrochemical luminescence detection
  • Method for enhancing luminous intensity of squamous-cell carcinoma antigen during electrochemical luminescence detection
  • Method for enhancing luminous intensity of squamous-cell carcinoma antigen during electrochemical luminescence detection

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Embodiment 1

[0035] 1. Experimental method

[0036] 1.1 Reagents

[0037] Selenium powder (Aladdin Reagent Company); Sodium borohydride (Shanghai Tianlian Fine Chemical Co., Ltd.); 3-mercaptopropionic acid (MPA), 1-ethyl-3-(3-dimethylaminopropylcarbotriimide) ( EDC), polyvinylpyrrolidone (PVP), FeCl 3 ·6H 2 O, 2-aminoterephthalic acid (2-NH 2 -BDC), dopamine (DA) (Aladdin reagent company), zinc chloride, sodium hydroxide, disodium hydrogen phosphate, sodium dihydrogen phosphate, N,N-dimethylformamide (DMF), ethanol (Shantou West, Guangdong Long Chemical Industry Co., Ltd.); sodium lauryl sulfate, bovine serum albumin (BSA) (Beijing Suo Laibao Technology Co., Ltd.); acetic acid (Guangdong Guanghua Technology Co., Ltd.); squamous cell carcinoma antigen (SCCA), mouse anti-SCCA antibody, alpha-fetoprotein (AFP), CA15-3, carcinoembryonic antigen (CEA) (Beijing Boaosen Biotechnology Co., Ltd.).

[0038] 1.2 Instruments

[0039] MPI-B multi-parameter chemical analysis detection system (Xi'a...

experiment example 1

[0072] Experimental example 1: The method of the present invention is applied to the analysis of actual specimen samples In order to investigate whether the electrochemiluminescence sensor constructed by the present invention can be used for the analysis of complex samples, the applicant used the sensor to analyze human serum samples. The human serum samples came from the Fifth People’s Hospital of Guilin City, and were directly analyzed according to the aforementioned immunoassay method without any pretreatment. The RSD is less than 7%, indicating that the electrochemiluminescent sensor constructed in the present invention is suitable for complex biological sample analysis.

[0073] Table 2: Determination results and recovery rates of SCCA in human serum samples (n=3)

[0074]

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Abstract

The invention discloses a method for enhancing the luminous intensity of a squamous-cell carcinoma antigen during electrochemical luminescence detection. The method mainly comprises a construction step of an electrochemical luminescence sensor, wherein the construction step comprises: preparing an SCCA solution with certain concentration, dripping the SCCA on a polymer molecular imprinting modified electrode for incubation, performing washing with water after the incubation is completed, then dripping a Fe-MIL-88-NH2 @ZnSe/Ab/BSA complex onto the electrode and incubating the same, performing washing with water after the incubation is completed, and drying to obtain the electrochemical luminescence sensor; and the prepared electrochemical luminescence sensor is placed in a PBS buffer solution containing a co-reactant to perform electrochemical luminescence detection, and then the luminous intensity of the SCCA during the electrochemical luminescence detection can be effectively. The electrochemical luminescence sensor constructed by the method disclosed by the invention has good selectivity and high sensitivity, and when the electrochemical luminescence sensor is applied to the electrochemical luminescence detection of the SCCA, the linear range of the method is effectively increased, and the detection sensitivity is improved.

Description

technical field [0001] The invention relates to a method for enhancing the luminescence intensity of a squamous cell carcinoma antigen during electrochemiluminescence detection, and belongs to the technical field of biological detection. Background technique [0002] Cervical cancer is the most common female cancer worldwide. Squamous cell carcinoma antigen (SCCA) is a subtype of glycoprotein with a molecular weight of about 45 to 55 kDa, which was isolated from cervical squamous cell carcinoma tissue for the first time (Wu L, Hu Y F, Sha Y H, Li W R, Yan T T, Wang S, Li X, Guo Z Y, Zhou J, Su XR. An “in-electrode”-type immunosensing strategy for the detection of squamouscell carcinoma antigen based on electrochemiluminescent AuNPs / g-C 3 N 4 nanocomposites [J]. Talanta, 2016, 160:247-255.). Initially, SCCA was found to be a good specific antigen for the diagnosis and monitoring of cervical squamous cell carcinoma. Later, SCCA was found to be associated with other types o...

Claims

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Application Information

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IPC IPC(8): G01N27/327G01N21/76
CPCG01N21/76G01N27/308G01N27/3277G01N27/3278
Inventor 邓必阳莫桂春郑向菲冯金素
Owner GUANGXI NORMAL UNIV
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